BACKGROUND AND OBJECTIVES: Chronic pain is a complex and multifactorial event. Very few research compare chronic pain according to the body region in teachers, a population with demanding workloads. The objective was to characterize the most uncomfortable chronic pain, reported by teachers, according to the body region. METHODS: A cross-sectional study conducted from 2012 to 2013 with K-12 teachers from the 20 largest state schools in Londrina (PR). Chronic pain (≥6-month duration) and the one that bothered the most were investigated. The Chi-square test (Yates) was used to compare the proportions of teachers with and without chronic pain, with a significance score of 5%. RESULTS: Among the 958 teachers, 408 (42.6%) reported chronic pain, and the most disturbing pain were located mainly in the upper limbs, head, lower limbs and lower back (n=321). Pain in the head region stood out for its longer duration, being classified as intense/unbearable and for strongly interfering with teachers' leisure and work. Pain in the upper and lower limbs frequently made it difficult for teachers to sleep. Teachers with low back pain had a greater proportion of leave of absence above 30 days compared to those with pain in other body sites. CONCLUSION: We observed differences in the most uncomfortable pain according to the regions of the body. Pain in the head region stood out for its duration, intense/unbearable intensity and for interfering in teachers' leisure/work in greater proportion.