Lyme disease (LD) is a natural focal zoonotic disease caused by Borrelia
burgdorferi, which is mainly transmitted through infected Ixodes
ricinus tick bites. The presence and abundance of ticks in various
habitats, the infectivity rate, as well as prolonged human exposure to ticks are
factors that may affect the infection risk as well as the incidence of LD. In recent
years, 20% to 25% of ticks infected with different borrelial species, as well as
about 5,300 citizens with LD, have been registered in the Belgrade area. Many of the
patients reported tick bites in city’s grassy areas. The aim of this study was to
assess the seroprevalence of B. burgdorferi in high-risk groups
(forestry workers and soldiers) in the Belgrade area, and to compare the results with
healthy blood donors. A two-step algorithm consisting of ELISA and Western blot tests
was used in the study. Immunoreactivity profiles were also compared between the
groups. The results obtained showed the seroprevalence to be 11.76% in the group of
forestry workers, 17.14% in the group of soldiers infected by tick bites and 8.57% in
the population of healthy blood donors. The highest IgM reactivity was detected
against the OspC protein, while IgG antibodies showed high reactivity against VlsE,
p19, p41, OspC, OspA and p17. Further investigations in this field are necessary in
humans and animals in order to improve protective and preventive measures against
LD.