Risk of type III secretion systems in burn patients with Pseudomonas aeruginosa wound infection: A systematic review and meta-analysis

Hasannejad‐Bibalan, Meysam; Jafari, Alireza; Sabati, Hoda; Goswami, Rajendra; Jafaryparvar, Zakiyeh; Sedaghat, Fatemeh; Ebrahim‐Saraie, Hadi Sedigh

doi:10.1016/j.burns.2020.04.024

Cited by 25 publications

(12 citation statements)

References 33 publications

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“…Moreover, while CF macrophages are known to have impaired phagocytosis and bacterial killing, this has been shown to be mainly due to the CF lung environment, inflammation, expression of dysfunctional cystic fibrosis transmembrane conductance regulator (CFTR) in macrophages, and other defects associated with CFTR dysfunction in macrophages and other immune cells ( 37 , 38 ). In burn wound patients, type III secretion system (T3SS) effectors such as ExoS and ExoT were also implicated in defective macrophage phagocytosis by disrupting host cell actin skeleton ( 39 ). In these cases, the loss of flagella, pili, and motility, as observed in our study, may not be the primary driver of reduced uptake of P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Adaptation to an Amoeba Host Leads to Pseudomonas aeruginosa Isolates with Attenuated Virulence

Leong

Poh

Williams

et al. 2022

Appl Environ Microbiol

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The opportunistic pathogen Pseudomonas aeruginosa , is ubiquitous in the environment, and in humans is capable of causing acute or chronic infections. In the natural environment, predation by bacterivorous protozoa represents a primary threat to bacteria. Here, we determined the impact of long-term exposure of P. aeruginosa to predation pressure. P. aeruginosa persisted when co-incubated with the bacterivorous Acanthamoeba castellanii for extended periods and produced genetic and phenotypic variants. Sequencing of late-stage amoeba-adapted P. aeruginosa isolates demonstrated single nucleotide polymorphisms within genes that encode known virulence factors and this correlated with a reduction in expression of virulence traits. Virulence towards the nematode, Caenorhabditis elegans , was attenuated in late-stage amoeba-adapted P. aeruginosa compared to early-stage amoeba-adapted and non-adapted counterparts. Further, late-stage amoeba-adapted P. aeruginosa showed increased competitive fitness and enhanced survival in amoeba as well as in macrophage and neutrophils. Interestingly, our findings indicate that the selection imposed by amoeba resulted in P. aeruginosa isolates with reduced virulence and enhanced fitness, similar to those recovered from chronic cystic fibrosis infections. Thus, predation by protozoa and long-term colonization of the human host may represent similar environments that select for similar losses of gene function. Importance Pseudomonas aeruginosa is an opportunistic pathogen that causes both acute infections in plants and animals, including humans, and chronic infections in immunocompromised and cystic fibrosis patients. This bacterium is commonly found in soils and water where bacteria are constantly under threat of being consumed by bacterial predators, e.g. protozoa. To escape being killed, bacteria have evolved a suite of mechanisms that protect them from being consumed or digested. Here, we examine the effect of long-term predation on the genotypes and phenotypes expressed by P. aeruginosa . We show that long term co-incubation with protozoa resulted in mutations that resulted in P. aeruginosa becoming less pathogenic. This is particularly interesting as we see similar mutations arise in bacteria associated with chronic infections. Importantly, the genetic and phenotypic traits possessed by late-stage amoeba-adapted P. aeruginosa are similar to what is observed for isolates obtained from chronic cystic fibrosis infections. This notable overlap in adaptation to different host types suggests similar selection pressures amongst host cell types as well as similar adaptation strategies.

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Section: Discussionmentioning

confidence: 99%

Adaptation to an Amoeba Host Leads to Pseudomonas aeruginosa Isolates with Attenuated Virulence

Leong

Poh

Williams

et al. 2022

Appl Environ Microbiol

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“…The importance of highly abundant virulence factors like iron-uptake- and secretion system-related genes in P. aeruginosa is comprehensively described in the literature [ 37 , 38 ]. Other genes reported in the literature like regulation-associated virulence genes, recently reported, were less frequently observed in our isolates [ 39 , 40 ].…”

Section: Discussionmentioning

confidence: 99%

Clonal Clusters, Molecular Resistance Mechanisms and Virulence Factors of Gram-Negative Bacteria Isolated from Chronic Wounds in Ghana

et al. 2021

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Wound infections are common medical problems in sub-Saharan Africa but data on the molecular epidemiology are rare. Within this study we assessed the clonal lineages, resistance genes and virulence factors of Gram-negative bacteria isolated from Ghanaian patients with chronic wounds. From a previous study, 49 Pseudomonas aeruginosa, 21 Klebsiella pneumoniae complex members and 12 Escherichia coli were subjected to whole genome sequencing. Sequence analysis indicated high clonal diversity with only nine P. aeruginosa clusters comprising two strains each and one E. coli cluster comprising three strains with high phylogenetic relationship suggesting nosocomial transmission. Acquired beta-lactamase genes were observed in some isolates next to a broad spectrum of additional genetic resistance determinants. Phenotypical expression of extended-spectrum beta-lactamase activity in the Enterobacterales was associated with blaCTX-M-15 genes, which are frequent in Ghana. Frequently recorded virulence genes comprised genes related to invasion and iron-uptake in E. coli, genes related to adherence, iron-uptake, secretion systems and antiphagocytosis in P. aeruginosa and genes related to adherence, biofilm formation, immune evasion, iron-uptake and secretion systems in K. pneumonia complex. In summary, the study provides a piece in the puzzle of the molecular epidemiology of Gram-negative bacteria in chronic wounds in rural Ghana.

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“…Pseudomonas aeruginosa is an important opportunistic pathogen and a leading cause of hospital-acquired infections in patients with compromised immune defence, such as burn patients (Gonzalez et al 2016;Kamali et al 2020;Hasannejad-Bibalan et al 2021). Besides planktonic form, this bacterium is able to form biofilms.…”

Section: Introductionmentioning

confidence: 99%

“…2020; Hasannejad‐Bibalan et al . 2021). Besides planktonic form, this bacterium is able to form biofilms.…”

Section: Introductionmentioning

confidence: 99%

In vitro activity of honey, total alkaloids of Sophora alopecuroides and matrine alone and in combination with antibiotics against multidrug-resistant Pseudomonas aeruginosa isolates

Jaktaji

Koochaki

2022

Letters in Applied Microbiology

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Natural products, including honey, total alkaloids of Sophora alopecuroides (TASA) and matrine have been used in combination with antibiotics against various pathogenic bacteria. However, there are limited data on the antibacterial activity of these natural products in combination against multidrug‐resistant Pseudomonas aeruginosa strains. The in vitro activity of honey, TASA and matrine alone and in combination with antibiotics against P. aeruginosa isolates was investigated. In this study, four biofilm‐producing P. aeruginosa isolates, which were resistant to multiple antibiotics, were used. These natural products were not the most effective single agent against four isolates. The fractional inhibitory concentration index method revealed the synergistic effect of matrine and TASA‐honey in combination with ciprofloxacin (Cip) against all tested isolates. When these combinations were used, the resistance of isolates to Cip was decreased significantly (six to eightfold reduction in the minimum inhibitory concentration of Cip. The disk diffusion method showed that all isolates were resistant to β‐lactams. Combinations of these antibiotics with TASA and matrine changed slightly the activity of either antibiotic used as a single agent. All isolates produced metallo‐β‐lactamase enzymes (MBL). Pretreatment isolates with Cip‐matrine and Cip‐TASA‐honey resulted in a statistically downregulated expression of the mexA gene. These natural products can be used against overactivating MexAB‐OprM but not MBL‐producing P. aeruginosa isolates.

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Risk of type III secretion systems in burn patients with Pseudomonas aeruginosa wound infection: A systematic review and meta-analysis

Cited by 25 publications

References 33 publications

Adaptation to an Amoeba Host Leads to Pseudomonas aeruginosa Isolates with Attenuated Virulence

Adaptation to an Amoeba Host Leads to Pseudomonas aeruginosa Isolates with Attenuated Virulence

Clonal Clusters, Molecular Resistance Mechanisms and Virulence Factors of Gram-Negative Bacteria Isolated from Chronic Wounds in Ghana

In vitro activity of honey, total alkaloids of Sophora alopecuroides and matrine alone and in combination with antibiotics against multidrug-resistant Pseudomonas aeruginosa isolates

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