This study explored the relationship between occupational health risk perception and job satisfaction. Based on the job demand-resources model and resource conservation theory, eight hypotheses were proposed in this study. In a survey of 237 production line workers and managers, we found that perceived occupational health risks significantly negatively affected job satisfaction. Both work stress and organizational commitment mediate the relationships between perceived occupational health risks and job satisfaction. We also examined whether safety culture could weaken the negative impact of perceived occupational health risks on job satisfaction. However, the results of our study did not support this hypothesis. This study not only helped managers to realize the hazards of occupational health risks, but also encouraged employees to actively participate in safety construction and pay attention to their own health. In addition, we also put forward some targeted intervention measures to reduce the negative impact of perceived occupational health risks on job satisfaction. Therefore, this study had certain practical implications.