RNA binding proteins involved in regulation of protein synthesis to initiate biogenesis of secondary tumor in hepatocellular carcinoma in mice

Abstract

Background The tumor microenvironment (TM) in close contact with cancer cells is highly related to tumor growth and cancer metastasis. This study is to explore the biogenesis mechanism of a secondary hepatocellular carcinoma (HCC) based on the function of RNA binding proteins (RBPs)-encoding genes in the physiological microenvironment (PM). Methods The healthy and HCC mice were used to isolate the PM, pre-tumor microenvironment (PTM), and TM. The samples were analyzed using the technology of RNA-seq and bioinformatics. The differentially expressed RBPs-encoding genes (DERs) and differentially expressed DERs-associated genes (DEDs) were screened to undergo GO and KEGG analysis. Results 18 DERs and DEDs were identified in the PTM vs. PM, 87 in the TM vs. PTM, and 87 in the TM vs. PM. Those DERs and DEDs participated in the regulation of gene expression at the levels of chromatin conformation, gene activation and silencing, splicing and degradation of mRNA, biogenesis of piRNA and miRNA, ribosome assemble, and translation of proteins. Conclusion The genes encoding RBPs and the relevant genes are involved in the transformation from PM to PTM, then constructing the TM by regulating protein synthesis. This regulation included whole process of biological genetic information transmission from chromatin conformation to gene activation and silencing to mRNA splicing to ribosome assemble to translation of proteins and degradation of mRNA. The abnormality of those functions in the organic microenvironments promoted the metastasis of HCC and initiated the biogenesis of a secondary HCC in a PM when the PM encountered the invasion of cancer cells.

PIWI-Interacting RNAs (piRNAs): Promising Applications as Emerging Biomarkers for Digestive System Cancer

“…RNA-binding proteins are also inextricably linked with HCC. Li et al (2020) found that RNA-binding proteins help transform the physiological microenvironment into the tumor microenvironment by regulating protein synthesis, thus initiating the biogenesis of secondary mouse HCC. PIWIL1 (also known as HIWI) is a member of the PIWI subfamily.…”

Exon Array Analysis to Identify Diethyl-nitrosamine Differentially Regulated and Alternately Spliced Genes in Early Liver Carcinogenesis in the Transgenic Mouse ATT-myc Model

“…In this analysis, we looked at both gene up-regulation and spliced genes in the livers of NDEA-treated mice and found 321 controlled groups in the GOMolFn of early tumorigenesis in att-myc mice treated with NDEA, with the top 30 up-regulation groups discussed in Table 7. The upregulation of RNA binding to protein involved in the regulation of protein synthesis to initiate biogenesis of the secondary tumor in hepatocellular carcinoma in mice [23].…”

Analysis of the Upregulated Expression Mechanism of Apoptotic Chromatin Condensation Inducer 1 in Hepatocellular Carcinoma Based on Bioinformatics

“…One hundred healthy mice aged 6-8 weeks and weighing 25-30 g were used to construct the mouse model of HCC according to the previous method in our laboratory. 11 The tumor tissues (set up as group “H”) and paracancerous liver tissues (set up as group “C”) were collected separately, as well as liver tissues from control mice injected with saline (set up as group “OO”). Three biological replicates of 100 mg of each sample were taken, and total RNA was extracted using a RNA extraction kit and sent to BGI Genomics Co., Ltd. (hereinafter referred to as “Huada Gene”).…”