RNA Granules in Germ Cells

Voronina, Ekaterina; Seydoux, Géraldine; Sassone–Corsi, Paolo; Nagamori, Ippei

doi:10.1101/cshperspect.a002774

Cited by 340 publications

(361 citation statements)

References 259 publications

(307 reference statements)

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“…Most of these factors were shown to be essential in piRNA amplification and transposon silencing. 2,3,51 The perinuclear granules positive for Vasa2 found throughout the development of Nematostella (Fig. 5) are highly reminiscent of nuage.…”

Section: Discussionmentioning

confidence: 99%

“…5D, arrowhead) suggests the association of Nematostella Vasa2 with the nuclear envelope, as in bilaterian model systems. 51 In Cnidaria, nuage has been identified in Clytia hemisphaerica in growing oocytes and in i-cells and epithelial stem cells of Hydra. 25,28,33 Our data confirm the notion that the association of the piRNA pathway to nuage is ancestral.…”

Section: Discussionmentioning

confidence: 99%

“…5,28,51 Besides Vasa, nuage contains several other conserved components such as Nanos, Mael, PIWI and Tudor proteins. Most of these factors were shown to be essential in piRNA amplification and transposon silencing.…”

Section: Discussionmentioning

confidence: 99%

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Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

et al. 2017

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PIWI-interacting RNAs (piRNAs) and associated proteins comprise a conserved pathway for silencing transposons in metazoan germlines. piRNA pathway components are also expressed in multipotent somatic stem cells in various organisms. piRNA functions have been extensively explored in bilaterian model systems, however, comprehensive studies in non-bilaterian phyla remain limited. Here we investigate the piRNA pathway during the development of Nematostella vectensis, a well-established model system belonging to Cnidaria, the sister group to Bilateria. To date, no population of somatic stem cells has been identified in this organism, despite its long life-span and regenerative capacities that require a constant cell-renewal. We show that Nematostella piRNA pathway components are broadly expressed in early developmental stages, while piRNAs themselves show differential expression, suggesting specific developmental roles of distinct piRNA families. In adults, piRNA associated proteins are enriched in the germline but also expressed in somatic cells, indicating putative stem cell properties. Furthermore, we provide experimental evidence that Nematostella piRNAs cleave transposable elements as well as protein-coding genes. Our results demonstrate that somatic expression of piRNA associated proteins as well as the roles of piRNAs in transposon repression and gene regulation are likely ancestral features that evolved before the split between Cnidaria and Bilateria.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

et al. 2017

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“…For instance, germ granules (subtypes of which include P-granules, nuage, chromatoid bodies, intermitochondrial cement, and sponge bodies) are usually defined as cytoplasmic mRNP foci present in germ cells, which often contain the RNA helicase Vasa, 4 and are implicated in storage and localization of mRNAs. The developmental stage, cellular localization, composition, and model organism studied further dictates their sub-classification.…”

Section: Diversity and Similarity Among Different Mrnp Granule Typesmentioning

confidence: 99%

“…In eukaryotes, nontranslating mRNPs often assemble together into visible cytoplasmic structures that lack a limiting membrane, termed mRNP granules. Specific examples of these include P-bodies, 1,2 stress granules, 2,3 germ granules, 4 and neuronal transport granules 5 (Fig. 1).…”

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confidence: 99%

mRNP granules

2014

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An in vivo proteomic analysis of the Me31B interactome in Drosophila germ granules

et al. 2017

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Drosophila Me31B is a conserved protein of germ granules, ribonucleoprotein complexes essential for germ cell development. Me31B post-transcriptionally regulates mRNAs by interacting with other germ granule proteins. However, a Me31B interactome is lacking. Here, we use an in vivo proteomics approach to show that the Me31B interactome contains polypeptides from four functional groups: RNA regulatory proteins, glycolytic enzymes, cytoskeleton/motor proteins, and germ plasm components. We further show that Me31B likely colocalizes with the germ plasm components Tudor (Tud), Vasa, and Aubergine in the nuage and germ plasm and provide evidence that Me31B may directly bind to Tud in a symmetrically dimethylated arginine-dependent manner. Our study supports the role of Me31B in RNA regulation and suggests its novel roles in germ granule assembly and function.

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RNA Granules in Germ Cells

Cited by 340 publications

References 259 publications

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

Characterization of the piRNA pathway during development of the sea anemone Nematostella vectensis

mRNP granules

An in vivo proteomic analysis of the Me31B interactome in Drosophila germ granules

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