RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

Lee, Dae‐Weon; Shrestha, Sony; Kim, A. Young; Park, Seok Joo; Yang, Chang Yeol; Kim, Yonggyun; Koh, Young Ho

doi:10.1016/j.ibmb.2011.01.001

Cited by 50 publications

(40 citation statements)

References 34 publications

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“…Although, concentrations required to stimulate pheromone biosynthesis in vitro in Helicoverpa armigera showed that PBAN was active at 0.5 nM and the other peptides at significantly higher concentrations (Stern et al, 2007). PBAN-receptors have been characterized from several moths including B. mori (Hull et al, 2004), Heliothis virescens (Kim et al, 2008), and Plutella xylostella (Lee et al, 2011). These studies indicate that PBAN will activate receptors at concentrations in the low nanomolar range.…”

Section: Pban-receptormentioning

confidence: 99%

“…Allocating these characteristics may be too premature until more evidence becomes available as to the involvement of cyclic-AMP in the signal transduction of other PBAN-receptor subtypes. For example, PBAN induces calcium elevations by the P. xyllostella PBAN-receptor, which also has a short C -terminal tail; however cyclic-AMP levels were not analyzed in the P. xyllostella study (Lee et al, 2011). …”

Section: Pban Mode Of Actionmentioning

confidence: 99%

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Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths

Jurenka¹,

Rafaeli²

2011

Front. Endocrin.

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Both males and females of heliothine moths utilize sex-pheromones during the mating process. Females produce and release a sex pheromone for the long–range attraction of males for mating. Production of sex pheromone in females is controlled by the peptide hormone (pheromone biosynthesis activating neuropeptide, PBAN). This review will highlight what is known about the role PBAN plays in controlling pheromone production in female moths. Male moths produce compounds associated with a hairpencil structure associated with the aedaegus that are used as short-range aphrodisiacs during the mating process. We will discuss the role that PBAN plays in regulating male production of hairpencil pheromones.

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Section: Pban-receptormentioning

confidence: 99%

Section: Pban Mode Of Actionmentioning

confidence: 99%

Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths

Jurenka¹,

Rafaeli²

2011

Front. Endocrin.

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“…However, extensive insecticide application has led to a rapid increase in its resistance to not only chemical pesticides but also biological pesticides, including pyrethroids, agricultural antibiotics, and Bacillus thuringiensis (Bt) toxins, one of the most successful microbial insecticides used for pest population control (37). Molecular studies have been extensively conducted previously in P. xylostella, especially in terms of insect physiology and insecticide resistance, including data on chemosensory proteins (38), immunity and defense in insect (39), hormonal regulation (40), cuticle function (41), and the mechanisms of resistance to insecticide (42), especially to Bt toxins (43).…”

mentioning

confidence: 99%

“…Omics approaches started to be applied to study the physiological and toxicological changes at a global scale in the midgut. ESTs and cDNA microarray technology were used to study immune-inducible genes in P. xylostella (39). More recently, Xie et al (46) provided more insights into P. xylostella EST database to understand the molecular mechanism of insecticide resistance, using an Illumina-based transcriptome profiling technique.…”

mentioning

confidence: 99%

Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline

Zhu

Xie

Armengaud

et al. 2016

Molecular & Cellular Proteomics

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The diamondback moth, Plutella xylostella (L.), is the major cosmopolitan pest of brassica and other cruciferous crops. Its larval midgut is a dynamic tissue that interfaces with a wide variety of toxicological and physiological processes. The draft sequence of the P. xylostella genome was recently released, but its annotation remains challenging because of the low sequence coverage of this branch of life and the poor description of exon/intron splicing rules for these insects. Peptide sequencing by computational assignment of tandem mass spectra to genome sequence information provides an experimental independent approach for confirming or refuting protein predictions, a concept that has been termed proteogenomics. In this study, we carried out an in-depth proteogenomic analysis to complement genome annotation of P. xylostella larval midgut based on shotgun HPLC-ESI-MS/MS data by means of a multialgorithm pipeline. A total of 876,341 tandem mass spectra were searched against the predicted P. xylostella protein sequences and a whole-genome six-frame translation database. Based on a data set comprising 2694 novel genome search specific peptides, we discovered 439 novel protein-coding genes and corrected 128 existing gene models. To get the most accurate data to seed further insect genome annotation, more than half of the novel protein-coding genes, i.e. 235 over 439, were further validated after RT-PCR amplification and sequencing of the corresponding transcripts. Furthermore, we validated 53 novel alternative splicings. Finally, a total of 6764 proteins were identified, resulting in one of the most comprehensive proteogenomic study of a nonmodel animal. As the first tissue-specific proteogenomics analysis of P. xylostella, this study provides the fundamental basis for high-throughput proteomics and functional genomics approaches aimed at deciphering the molecular mechanisms of resistance and controlling this pest. Thousands of eukaryotic genomes have been sequenced with the rapid development of massive parallel sequencing technologies. The annotation of their genomic DNA sequences is a prerequisite to delineate conserved elements and understand the specific encoded functions. Today, conventional approaches used to identify protein-coding genes are highly dependent on predictions based on computational algorithms and homology searches against known proteins. Nonmodel organisms, such as most arthropods, are by nature distantly related to well-studied organisms (1). As a result, their genomes encode a large majority of proteins with little similarity to known proteins, challenging the annotation process. Much recent focus on computational gene finding is using transcript evidence to complement this predictionbased approach. Indeed, the use of information from deep sequencing of mRNA-derived cDNA libraries (RNA-seq) or expressed sequence tag (EST) 1 libraries can dramatically improve the genome annotation confidence (2-4). However, this analysis remains at the transcript level and cannot make a crystal-clear distinct...

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“…The PBAN receptor (PBANR) was first cloned from H. zea (20) as an ortholog of the vertebrate neuromedin U receptor (NMUR) (21)(22)(23)(24), which recognizes peptides with a C-terminal FRPRN-NH 2 motif such as neuromedin U (NMU) and neuromedin S (25,26). Since then, PBANRs from B. mori (27), Heliothis virescens (28), Manduca sexta (GenBank TM accession numbers FJ240221 to FJ240224), 5 Plutella xylostella (29), Helicoverpa armigera (30), Spodoptera littoralis (31), Spotoptera exigua (32), Pseudaletia separata (33), and Ostrinia nubilalis (34) have been identified. Functional analyses have shown that PBANR activation triggers an influx of extracellular Ca 2ϩ in isolated B. mori pheromone glands (35) as well as insect cells transiently expressing PBANR (20,27).…”

mentioning

confidence: 99%

Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor

Kawai

Katayama

Guo

et al. 2014

Journal of Biological Chemistry

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Background:The moth pheromone biosynthesis-activating neuropeptide (PBAN) and vertebrate neuromedin U (NMU) have a similar biologically essential C-terminal motif (FX 1 PRX 2 -NH 2 ). Results: Mutation data revealed important residues in the silkmoth PBAN receptor for ligand binding and signaling. Conclusion: Two glutamate residues conserved in the PBAN/NMU receptor family of GPCRs are responsible for ligand recognition. Significance: A novel ligand-receptor interaction is proposed for the PBAN/NMU family of neuropeptides and receptors.

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RNA interference of pheromone biosynthesis-activating neuropeptide receptor suppresses mating behavior by inhibiting sex pheromone production in Plutella xylostella (L.)

Cited by 50 publications

References 34 publications

Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths

Regulatory Role of PBAN in Sex Pheromone Biosynthesis of Heliothine Moths

Tissue-specific Proteogenomic Analysis of Plutella xylostella Larval Midgut Using a Multialgorithm Pipeline

Identification of Functionally Important Residues of the Silkmoth Pheromone Biosynthesis-activating Neuropeptide Receptor, an Insect Ortholog of the Vertebrate Neuromedin U Receptor

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