1985
DOI: 10.1002/j.1460-2075.1985.tb02336.x
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RNA processing in Neurospora crassa mitochondria: transfer RNAs punctuate a large precursor transcript.

Abstract: The pattern of transcripts arising from a large contiguous portion of the Neurospora crassa mitochondrial genome and the processing of these transcripts have been investigated. Evidence is presented for the transcription of a single, at least 12.5 kb, precursor RNA that contains sequences corresponding to the apocytochrome b (cob), tRNACys, cytochrome oxidase subunit 1 (CO I), tRNAArg and unidentified reading frame (URF) 1 genes. The two tRNA sequences serve as punctuation signals for the cleavage of this larg… Show more

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Cited by 54 publications
(27 citation statements)
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“…However, only the larger 2?3 kb transcript was notably overexpressed at 37˚C. The existence of both pre-processed and processed forms of mitochondrial mRNAs, including the COX1 transcript, has been described previously in several fungi (Agsteribbe & Hartog, 1987;Burger et al, 1985;Costanzo & Fox, 1990;Dyson et al, 1989;Grivell, 1989;Osinga et al, 1984;Tracy & Stern, 1995), plants (Barkan, 1988;Gray & Lovello, 1993;Tracy & Stern, 1995;Wolff & Kuck, 1996) and animals (Ojala et al, 1980;Tracy & Stern, 1995). These pre-processed or precursor RNAs comprise polycistronic transcripts that have not yet been processed into smaller, intermediate and mature forms of RNA.…”
Section: Discussionmentioning
confidence: 69%
See 1 more Smart Citation
“…However, only the larger 2?3 kb transcript was notably overexpressed at 37˚C. The existence of both pre-processed and processed forms of mitochondrial mRNAs, including the COX1 transcript, has been described previously in several fungi (Agsteribbe & Hartog, 1987;Burger et al, 1985;Costanzo & Fox, 1990;Dyson et al, 1989;Grivell, 1989;Osinga et al, 1984;Tracy & Stern, 1995), plants (Barkan, 1988;Gray & Lovello, 1993;Tracy & Stern, 1995;Wolff & Kuck, 1996) and animals (Ojala et al, 1980;Tracy & Stern, 1995). These pre-processed or precursor RNAs comprise polycistronic transcripts that have not yet been processed into smaller, intermediate and mature forms of RNA.…”
Section: Discussionmentioning
confidence: 69%
“…This common occurrence of transcriptional units in mitochondrial DNA from a variety of plants (Barkan, 1988;Tracy & Stern, 1995;Wolff & Kuck, 1996) and fungi (Agsteribbe & Hartog, 1987;Burger et al, 1985;Costanzo & Fox, 1990;Dyson et al, 1989;Grivell, 1989;Osinga et al, 1984;Tracy & Stern, 1995) implicates their role in facilitating the co-ordinate regulation of gene expression. In our study, the overexpression of a single transcriptional unit encoding ATP9, COX1 and ATP8 mRNAs in C. neoformans suggests that at elevated host temperatures the serotype A strain (H99) upregulates expression of this transcriptional unit.…”
Section: Discussionmentioning
confidence: 94%
“…In this respect, S. pombe more resembles animals (Tracy and Stern 1995), Candida parapsilosis (Nosek et al 2004), Aspergillus nidulans (Dyson et al 1989), N. crassa (Burger et al 1985;Kubelik et al 1990), the chlorophytic unicellular alga Prototheca wickerhamii (Wolff and Kück 1996), and the red alga Chondrus crispus (Richard et al 1998). This broad evolutionary distribution of species with only a few mitochondrial promoters suggests that S. pombe retained a more ancestral mode of transcription and RNA-processing, and that high mitochondrial promoter density is a secondary adaptation.…”
Section: Nonanucleotide Motif Promoters Mark Transcription Units On Smentioning
confidence: 99%
“…In polycistronic precursor RNAs, exonucleolytic cleavage of their 5Ј-and 3Ј-ends liberates flanking mRNA and rRNA molecules in a wide range of eukaryotes including S. cerevisiae, N. crassa, Aspergillus nidulans, and green and red algae (Burger et al 1985;Dyson et al 1989;Wolff and Kück 1996;Richard et al 1998). This mechanism of RNA-processing is known as the "tRNA punctuation model."…”
Section: Introductionmentioning
confidence: 99%
“…Considering the position of the tRNA genes, which are located in the vicinity of the maturation sites at the RNA level, it can be assumed that the processing of the precursor is, at least in part, achieved by the release of tRNA molecules. The excision of tRNAs has been described as an RNA maturation mechanism in a variety of organisms, including animals, fungi, and green algae (Ojala et al 1980(Ojala et al , 1981Burger et al 1985;Dyson et al 1989;Schäfer et al 2005;Wolff and Kück 1996).…”
Section: Discussionmentioning
confidence: 99%