RNA seq analysis of potato cyst nematode interactions with resistant and susceptible potato roots

Walter, Abigail J.; Willforss, Jakob; Lenman, Marit; Alexandersson, Erik; Andréasson, Erik

doi:10.1007/s10658-018-1474-z

Cited by 12 publications

(20 citation statements)

References 30 publications

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“…Activation of R proteins leads to the transcriptional reprogramming of cells leading to the activation of local and systemic defense responses. Transcriptome studies revealed insights in downstream signaling pathways involved in host-specific resistance to cyst nematodes ( Uehara et al, 2010 ; Walter et al, 2018 ). For instance, in resistant tomato harboring the Hero A gene, the salicylic acid (SA)-dependent pathogenesis-related protein 1 (PR-1) shows a markable increase in expression at 3 dpi upon G. rostochiensis infection compared to susceptible plants ( Uehara et al, 2010 ).…”

Section: Effector-triggered Defense Responses To Cyst Nematodesmentioning

confidence: 99%

“…This was not the case in resistant plants with an extra inserted NahG gene, which can prevent SA accumulation, indicating that SA plays a key role in Hero A-mediated resistance to cyst nematodes. Another transcriptome study performed on resistant potato containing the H1 gene shows upregulation of many genes after G. rostochiensis infection, including the tomato stress-responsive factor TSRF1 and a cysteine protease ( Walter et al, 2018 ). TSRF1 is an ethylene responsive factor, which can be upregulated by ethylene or SA treatment ( Zhang et al, 2004 ).…”

Section: Effector-triggered Defense Responses To Cyst Nematodesmentioning

confidence: 99%

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Molecular and Cellular Mechanisms Involved in Host-Specific Resistance to Cyst Nematodes in Crops

2021

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Cyst nematodes are able to infect a wide range of crop species and are regarded as a major threat in crop production. In response to invasion of cyst nematodes, plants activate their innate immune system to defend themselves by conferring basal and host-specific defense responses depending on the plant genotype. Basal defense is dependent on the detection of pathogen-associated molecular patterns (PAMPs) by pattern recognition receptors (PRRs), while host-specific defense mainly relies on the activation of canonical and non-canonical resistance (R) genes or quantitative trait loci (QTL). Currently, application of R genes and QTLs in crop species is a major approach to control cyst nematode in crop cultivation. However, emerging virulent cyst nematode field populations are threatening crop production due to host genetic selection by the application of a limited set of resistance genes in current crop cultivars. To counteract this problem, increased knowledge about the mechanisms involved in host-specific resistance mediated by R genes and QTLs to cyst nematodes is indispensable to improve their efficient and sustainable use in field crops. Despite the identification of an increasing number of resistance traits to cyst nematodes in various crops, the underlying genes and defense mechanisms are often unknown. In the last decade, indebt studies on the functioning of a number of cyst nematode R genes and QTLs have revealed novel insights in how plants respond to cyst nematode infection by the activation of host-specific defense responses. This review presents current knowledge of molecular and cellular mechanisms involved in the recognition of cyst nematodes, the activation of defense signaling and resistance response types mediated by R genes or QTLs. Finally, future directions for research are proposed to develop management strategies to better control cyst nematodes in crop cultivation.

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Section: Effector-triggered Defense Responses To Cyst Nematodesmentioning

confidence: 99%

Section: Effector-triggered Defense Responses To Cyst Nematodesmentioning

confidence: 99%

Molecular and Cellular Mechanisms Involved in Host-Specific Resistance to Cyst Nematodes in Crops

2021

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“…Germin and Lemir genes reached a higher value of RPKM after infection of Globodera pallida, compared to the susceptible variety. Germin (locus ST4.03ch01: 78647674-79648034) is an enzyme with oxalate oxidase activity, producing hydrogen peroxide (H2O2), toxic to pathogenic agents (Lane et al 1994, Caliskan 2000, Walter, et al 2018. On the other hand, Lemir has an anti-insect/anti pathogenic action, implicated in with defense against nematodes or other pathogens (Ryan 1990).…”

Section: Differential Gene Expression Using Cufflinks Wementioning

confidence: 99%

Transcriptomic profiling against Globodera pallida in resistant and susceptible potato roots

et al. 2020

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Globodera pallida is a white potato cyst nematode present in the Andes, which causes huge losses to Peruvian farmers. An RNA-seq analysis allowed the identification of candidate genes that could mediate resistance against this pathogen. Two varieties, “María Huanca” (Solanum andigena) clone resistant (CIP 279142.12) and “Chimbina Colorada” (Solanum chaucha) (CIP 701013) clone susceptible to G. pallida, were used to identify differentially expressed genes. Total RNA from roots was extracted 72 hours post inoculation with second stage juveniles. Sequencing was done using the Illumina Hiseq 2500 platform. Reads were screened for quality issues and then mapped to the reference potato genome (clone DM1-3516 R44 v4.03). Here, we report 27717 and 27750 genes expressed in the resistant and susceptible variety respectively. The comparative analysis of expression identified 100 candidate genes. 91 genes were associated with resistance to G. pallida with Fold Change ≥ 2 (p <0.05). The remaining 9 R genes had Fold Change ≤ 1. We show differences in the expression of an NBS-LRR protein similar to Gro1-8, genes linked to late blight and TMV virus resistance.

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“…RNA-seq profiling has been used to decipher potato responses to various abiotic [ 15 , 16 ] and biotic stresses [ 17 , 18 ] where large sets of genes and pathways associated with either biotic and abiotic stress were revealed. To date, most research has focused on potato gene expression in response to potato cyst nematodes [ 19 , 20 , 21 ] while potato responses to RKN infestation remain poorly understood. Here, we set out to evaluate the molecular basis of Potato-RKN compatible interaction, we employed RNA-Seq to analyze differential gene expression patterns in S. tuberosum cv.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Profiling of Potato (Solanum tuberosum L.) Responses to Root-Knot Nematode (Meloidogyne javanica) Infestation during A Compatible Interaction

2020

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Root-knot nematode (RKN) Meloidogyne javanica presents a great challenge to Solanaceae crops, including potato. In this study, we investigated transcriptional responses of potato roots during a compatible interaction with M. javanica. In this respect, differential gene expression of Solanum tuberosum cultivar (cv.) Mondial challenged with M. javanica at 0, 3 and 7 days post-inoculation (dpi) was profiled. In total, 4948 and 4484 genes were detected, respectively, as differentially expressed genes (DEGs) at 3 and 7 dpi. Functional annotation revealed that genes associated with metabolic processes were enriched, suggesting they might have an important role in M. javanica disease development. MapMan analysis revealed down-regulation of genes associated with pathogen perception and signaling suggesting interference with plant immunity system. Notably, delayed activation of pathogenesis-related genes, down-regulation of disease resistance genes, and activation of host antioxidant system contributed to a susceptible response. Nematode infestation suppressed ethylene (ET) and jasmonic acid (JA) signaling pathway hindering JA/ET responsive genes associated with defense. Genes related to cell wall modification were differentially regulated while transport-related genes were up-regulated, facilitating the formation of nematode feeding sites (NFSs). Several families of transcription factors (TFs) were differentially regulated by M. javanica infestation. Suggesting that TFs play an indispensable role in physiological adaptation for successful M. javanica disease development. This genome-wide analysis reveals the molecular regulatory networks in potato roots which are potentially manipulated by M. javanica. Being the first study analyzing transcriptome profiling of M. javanica-diseased potato, it provides unparalleled insight into the mechanism underlying disease development.

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RNA seq analysis of potato cyst nematode interactions with resistant and susceptible potato roots

Cited by 12 publications

References 30 publications

Molecular and Cellular Mechanisms Involved in Host-Specific Resistance to Cyst Nematodes in Crops

Molecular and Cellular Mechanisms Involved in Host-Specific Resistance to Cyst Nematodes in Crops

Transcriptomic profiling against Globodera pallida in resistant and susceptible potato roots

Transcriptome Profiling of Potato (Solanum tuberosum L.) Responses to Root-Knot Nematode (Meloidogyne javanica) Infestation during A Compatible Interaction

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