RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius

Gerin, Donato; Angelini, Rita Milvia De Miccolis; Pollastro, Stefania; Faretra, F.

doi:10.1371/journal.pone.0147089

Cited by 27 publications

(42 citation statements)

References 62 publications

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“…ochraceus. The transcriptome analysis of A. carbonarius strains under OTA inducing conditions showed that AcOTApks, AcOTAnrps, AcOTAp450, and AcOTAhal were highly up-regulated in OTA inducing conditions and all were co-expressed in addition to the AcOTAbZIP (Gerin et al, 2016) supporting the fact that the cluster 38 is likely the OTA gen cluster in A. carbonarius.…”

Section: Discussionmentioning

confidence: 88%

“…carbonarius strains under OTA induction conditions (Gerin et al, 2016). In the nonochratoxigenic strains an oxalacetate acetylhidrolase (ID 158065) was down-regulated.…”

Section: Differential Gene Expression and Functional Analysismentioning

confidence: 93%

“…In A. carbonarius a hypothetical OTA gene cluster (cluster 38) has been described (Gerin et al, 2016). This cluster contained 21 genes and only some have been correlated with OTA biosynthesis.…”

Section: Introductionmentioning

confidence: 99%

“…Two other genes located in the same genomic region have been described: the cytochrome p450 monooxygenase AcOTAp450 and the transcription factor AcOTAbZIP. It has been shown that under OTA induction conditions all these genes were up-regulated and co-expressed (Gerin et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

Castellá

Bragulat

Cigliano

et al. 2020

International Journal of Food Microbiology

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Aspergillus carbonarius consistently produces large amounts of ochratoxin A (OTA), a mycotoxin with nephrotoxic effects on animals and humans. In the present study, we analyzed the transcriptional changes associated to OTA production in three atypical non-ochratoxigenic strains of A. carbonarius. In addition, in vitro interactions between ochratoxigenic strains of A. carbonarius and A. niger and non-ochratoxigenic strains of A. carbonarius and A. tubingensis were studied in order to evaluate their potential for controlling OTA production. RNA-seq analysis revealed that there are 696 differentially expressed genes identified in the three non-OTA producing strains, including 280 upregulated and 333 down-regulated genes. A functional and gene ontology enrichment analysis revealed that the processes related to metabolic and oxidation processes, associated with functions such oxidoreductase and hydrolase activity were down regulated. All the genes related with OTA biosynthesis in A. carbonarius were the most down-regulated genes in non-ochratoxigenic strains. We also showed that these strains possess a deleterious mutation in the AcOTApks gene required for OTA biosynthesis. Moreover, one of these strains gave the best control of OTA production resulting in an OTA reduction of 98-100% in co-inoculation with an ochratoxigenic strain of A. niger and an OTA reduction of 79-89% with an ochratoxigenic strain of A. carbonarius. Results of this study provided novel insights into the knowledge of the OTA biosynthetic pathway in these non-ochratoxigenic wild strains, and showed the biocontrol potential of these strains.

show abstract

Section: Discussionmentioning

confidence: 88%

“…carbonarius strains under OTA induction conditions (Gerin et al, 2016). In the nonochratoxigenic strains an oxalacetate acetylhidrolase (ID 158065) was down-regulated.…”

Section: Differential Gene Expression and Functional Analysismentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

Castellá

Bragulat

Cigliano

et al. 2020

International Journal of Food Microbiology

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“…The halogenase gene was associated with OTA biosynthesis by use of differentialdisplay reverse transcriptase PCR (RT-PCR) with P. nordicum grown on minimal media either suitable or inadequate for OTA production (15). Transcriptome sequencing (RNA-Seq) and reverse transcriptase quantitative PCR (RT-qPCR) analysis were used to associate a PKS, an NRPS, and a chloroperoxidase with the OTA biosynthetic process (38).…”

Section: Discussionmentioning

confidence: 99%

A Consensus Ochratoxin A Biosynthetic Pathway: Insights from the Genome Sequence of Aspergillus ochraceus and a Comparative Genomic Analysis

Wang

et al. 2018

Appl Environ Microbiol

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Ochratoxin A (OTA) is a toxic secondary metabolite produced by and species that widely contaminate food and feed. We sequenced and assembled the complete ∼37 Mb genome of fc-1, a well-known producer of OTA. Key genes of the OTA biosynthetic pathway were identified by comparative genomic analyses with five other sequenced OTA-producing fungi,, ,, , and OTA production was completely inhibited in the deletion mutants (- and ), and OTA biosynthesis was restored by feeding a post-block substrate to the corresponding mutant. The OTA biosynthetic pathway was unblocked with the addition of heterologously expressed halogenase in the mutant. OTA biosynthesis begins with PKS (), utilizing acetyl-CoA and malonyl-CoA to synthesize 7-methylmellein, which is oxidized to OTβ by cytochrome P450 monooxygenase (). OTβ and L-β-phenylalanine are combined by NRPS () to form an amide bond to synthesize OTB. Finally, OTB is chlorinated by halogenase () to OTA. The - genes were expressed at low levels in the mutant. A second regulator, otaR2, which is adjacent to the biosynthetic gene, could modulate only the expression of, and Thus, we have identified a consensus OTA biosynthetic pathway that can be used to prevent and control OTA synthesis, and to understand variation and production of the intermediate components in the biosynthetic pathway. Ochratoxin A (OTA) is a significant mycotoxin that contaminates cereal products, coffee, grapes, wine, cheese and meat. OTA is nephrotoxic, carcinogenic, teratogenic and immunotoxic. OTA contamination is a serious threat to food safety, endangers human health and can cause huge economic losses. At present, more than 20 species of the genera and are known to produce OTA. Here, we demonstrate that a consensus OTA biosynthetic pathway exists in all OTA-producing fungi and that is encoded by a gene cluster containing four highly conserved biosynthetic genes and a bZIP transcription factor.

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Molecular Identification and Detection of Foodborne and Feedborne Mycotoxigenic Fungi

Midorikawa

Miller

Bittencourt

2018

Molecular Techniques in Food Biology

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RNA-Seq Reveals OTA-Related Gene Transcriptional Changes in Aspergillus carbonarius

Cited by 27 publications

References 62 publications

Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

Transcriptome analysis of non-ochratoxigenic Aspergillus carbonarius strains and interactions between some black aspergilli species

A Consensus Ochratoxin A Biosynthetic Pathway: Insights from the Genome Sequence of Aspergillus ochraceus and a Comparative Genomic Analysis

Molecular Identification and Detection of Foodborne and Feedborne Mycotoxigenic Fungi

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