2016
DOI: 10.1079/pavsnnr201611027
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RNA silencing approaches for identifying pathogenicity and virulence elements towards engineering crop resistance to plant pathogenic fungi.

Abstract: Over the past several years, RNA interference (RNAi) has emerged as a powerful genetic tool that is being exploited not only in fundamental research for the assessment of gene functions, but also in various fields of applied research in medicine and agriculture. In plants, RNAi strategies have the potential to allow manipulation of various aspects of food quality and nutritional content, but also for plant protection. In the study of plant-fungus interactions, a comprehensive understanding of pathogenic mechan… Show more

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Cited by 9 publications
(9 citation statements)
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References 87 publications
(126 reference statements)
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“…This technique, termed post‐transcriptional gene silencing (PTGS) in plants, involves the endonucleolytic cleavage of double‐stranded RNA (dsRNA) into small, ~20–23 nucleotide (nt)‐long, small interfering RNA (siRNA) duplexes by the Dicer‐like (DCL) enzymes which along with their associated proteins control the expression of genetic information (Baulcombe, ; Wilson and Doudna, ). An RNAi‐based approach referred to as host‐induced gene silencing (HIGS) or host‐delivered RNAi (HD‐RNAi) in which small RNAs are produced by the host plant to target parasite transcripts has provided a promising strategy for improving plant resistance against pathogens, including fungi and oomycetes (Andrade et al ., ; Govindarajulu et al ., ; Huang et al ., ; Jahan et al ., ; Koch and Kogel, ; Koch et al ., ; Nowara et al ., ; Panwar et al ., , ; Song and Thomma, ; Yin et al ., ). When targeted against crucial pathogenicity genes, HD‐RNAi can potentially be developed as a genetic method to curb pathogen virulence for pesticide‐free disease control in crop plants.…”
Section: Introductionmentioning
confidence: 99%
“…This technique, termed post‐transcriptional gene silencing (PTGS) in plants, involves the endonucleolytic cleavage of double‐stranded RNA (dsRNA) into small, ~20–23 nucleotide (nt)‐long, small interfering RNA (siRNA) duplexes by the Dicer‐like (DCL) enzymes which along with their associated proteins control the expression of genetic information (Baulcombe, ; Wilson and Doudna, ). An RNAi‐based approach referred to as host‐induced gene silencing (HIGS) or host‐delivered RNAi (HD‐RNAi) in which small RNAs are produced by the host plant to target parasite transcripts has provided a promising strategy for improving plant resistance against pathogens, including fungi and oomycetes (Andrade et al ., ; Govindarajulu et al ., ; Huang et al ., ; Jahan et al ., ; Koch and Kogel, ; Koch et al ., ; Nowara et al ., ; Panwar et al ., , ; Song and Thomma, ; Yin et al ., ). When targeted against crucial pathogenicity genes, HD‐RNAi can potentially be developed as a genetic method to curb pathogen virulence for pesticide‐free disease control in crop plants.…”
Section: Introductionmentioning
confidence: 99%
“…24 This approach utilised a RNAi vector containing an intron sequence between two inversely oriented and self-complementary target sequences, which when expressed, generate a dsRNA molecule with a hairpin structure. 26 In plants, and some animals, locally initiated gene silencing can spread to other parts of the organisms, through systemic or cell-to-cell transport of the silencing signal. In plants, the silencing signal is suggested to be transmitted long range by the phloem, following source to sink dynamics.…”
Section: Rnai Mechanismsmentioning
confidence: 99%
“…By carefully designing the sequences to be used for HIGS and targeting the same gene in different fungal species, there is a strong possibility that broad spectrum control could be achieved. 26 Exploring the vast data sets of genomic and transcriptomic information during the initial construct design phase in any project should decrease the chances of off-target silencing of unintended genes in the host plants, as well as in the beneficial plant-associated organisms, such as mycorrhizas, rhizobia and biocontrol species, like Trichoderma species. As a example, a HIGS study conducted in maize to downregulate aflatoxin biosynthesis in Aspergillus flavus caused stunting and reduced kernel placement in transgenic plants, potentially due to off-target silencing of other genes.…”
Section: Challenges and Benefits Of Using Sigs And Higs To Control Fhmentioning
confidence: 99%
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