RNAi activation with homologous and heterologous sequences that induce resistance against the begomovirus Pepper golden mosaic virus (PepGMV)

Vargas-Salinas, Mayela; Medina-Hernández, Diana; Arcos-Ortega, Guadalupe Fabiola; Luis-Villaseñor, Irasema E.; Holguín-Peña, Ramón Jaime

doi:10.1007/s13205-021-02653-7

Cited by 3 publications

(3 citation statements)

References 55 publications

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“…AT1G69310 ( AtWRKY57 ), the ortholog of Solyc04g072070 ( SlWRKY55 ), plays a key role in the convergence of auxin signaling and jasmonic acid-mediated signaling during jasmonic acid-induced foliar senescence ( Caicedo et al., 2021 ); AT2G46400 ( ATWRKY46 ), the ortholog of Solyc12g056750 ( SlWRKY61 ), mediates cold tolerance ( Zhu et al., 2004 ); AT2G44745 ( AtWRKY13 ), the ortholog of Solyc02g071130 ( SlWRKY71 ), is negatively regulated during Pectobacterium carotovorum infiltration ( Tang and Liu, 2021 ); and AT3G01970 ( AtWRKY45 ), the ortholog of Solyc05g015850 ( SlWRKY75 ), enhances tolerance to salt stress and phosphate starvation ( Li et al., 2019 ). Similarly, in the bHLH family, Solyc01g086870 ( SlbHLH076 ) is highly expressed during Cd stress and its ortholog in Arabidopsis (AT4G29100) is a defense-related gene ( Vargas-Salinas et al., 2021 ). AT2G43140, the ortholog of Solyc08g062780 ( SlbHLH089 ), is highly expressed during Cd stress, which is in agreement with previous investigations that AT2G43140 ( AtbHLH129 ) is most likely to be involved in stress response ( Meng et al., 2020 ) and that AT3G19860 ( AtbHLH121 ), the ortholog of the highly expressed Solyc01g102300 ( SlbHLH006 ) gene, is found to act as a key regulator of the iron-deficient signaling pathway ( Ying, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide annotation and expression analysis of WRKY and bHLH transcriptional factor families reveal their involvement under cadmium stress in tomato (Solanum lycopersicum L.)

et al. 2023

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The WRKY and bHLH transcription factors have been implicated in the regulation of gene expression during various physiological processes in plants, especially in plant stress responses. However, little information about the heavy metal-responsive SlWRKY and SlbHLH in tomato (Solanum lycopersicum) is available. We performed a genome-wide investigation for these two TF families in S. lycopersicum and determined their role in cadmium (Cd) stress tolerance. Furthermore, ortholog analysis with the Arabidopsis genome led to classifying WRKY and bHLH ortholog genes into nine and 11 clusters, respectively. The comparative phylogenetic analysis revealed duplication events and gene loss in Arabidopsis and S. lycopersicum, which occurred during evolution both before and after the last common ancestor of the two species. Orthologous relationships are also supported by additional evidence, such as gene structure, conserved motif compositions, and protein–protein interaction networks for the majority of genes, suggesting their similar functions. A comprehensive transcriptomics analysis revealed that both WRKY and bHLH genes were differentially expressed in response to cadmium stress as compared with control plants. A gene ontology analysis revealed that most WRKYs and bHLHs are DNA-binding essential proteins that regulate gene expression positively and negatively. Analyses of interaction networks revealed that both WRKYs and bHLHs mediate networks implicated in several stress-signaling pathways. The findings of this work may help us to comprehend the intricate transcriptional control of WRKY and bHLH genes and identify potential stress-responsive genes relevant to tomato genetic improvement. Moreover, identifying heavy metal stress-responsive WRKY and bHLH genes in S. lycopersicum will provide fundamental insights for developing new heavy metal stress-tolerant varieties of tomato crops.

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Section: Discussionmentioning

confidence: 99%

Genome-wide annotation and expression analysis of WRKY and bHLH transcriptional factor families reveal their involvement under cadmium stress in tomato (Solanum lycopersicum L.)

et al. 2023

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“…Also, expression profiling of genes in TYLCV infected tomato showed that multiple RNAi core genes including SlDCL1/2/3, SlRDR2, SlRDR6, and five AGO genes (SlAGO1a, 1b, 4a, 4b, and 5) are triggered with high level expression in response to virus infection (Bai et al, 2012). In Nicotiana benthamiana, expression of repeat sequence fragments from both Pepper golden mosaic virus (PepGMV) and Tomato chino La Paz virus (ToChLPV) are able to upregulate DCL2/3/4, AGO1/2/3, AGO7, AGO10, and RDR6 transcripts (Vargas-Salinas et al, 2021). AGO1 mRNA level is elevated in Cymbidium ringspot virus (CymRSV) infected plants (Havelda et al, 2008).…”

Section: The Effect Of Viral Infection On Rnai-related Gene Expressionmentioning

confidence: 99%

Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions

et al. 2022

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As sessile organisms, plants encounter diverse invasions from pathogens including viruses. To survive and thrive, plants have evolved multilayered defense mechanisms to combat virus infection. RNAi, also known as RNA silencing, is an across-kingdom innate immunity and gene regulatory machinery. Molecular framework and crucial roles of RNAi in antiviral defense have been well-characterized. However, it is largely unknown that how RNAi is transcriptionally regulated to initiate, maintain and enhance cellular silencing under normal or stress conditions. Recently, insights into the transcriptional and post-transcriptional regulation of RNAi-related genes in different physiological processes have been emerging. In this review, we integrate these new findings to provide updated views on how plants modulate RNAi machinery at the (post-) transcriptional level to respond to virus infection.

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“…One strand of the siRNA duplex is then loaded into an RNA-induced silencing complex (RISC), which contains RNase H-like Argonautes (e.g., AGO1/2), and guides AGO to cleave homologous target RNAs, such as viral RNAs (Fang and Qi, 2016;Wilson and Doudna, 2013). Interestingly, viral infection often activates and upregulates expression of multiple RNAi-related genes in plants (Bai et al, 2012;Qin et al, 2018;Vargas-Salinas et al, 2021), but its initial cue is largely unknown. Moreover, it also remains unclear how RNAi genes are transcriptionally regulated, although posttranscriptional control for some RNAi genes has been described (Allen et al, 2005;Yang et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants

Wang

Gong

et al. 2021

Cell Host & Microbe

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RNAi activation with homologous and heterologous sequences that induce resistance against the begomovirus Pepper golden mosaic virus (PepGMV)

Cited by 3 publications

References 55 publications

Genome-wide annotation and expression analysis of WRKY and bHLH transcriptional factor families reveal their involvement under cadmium stress in tomato (Solanum lycopersicum L.)

Genome-wide annotation and expression analysis of WRKY and bHLH transcriptional factor families reveal their involvement under cadmium stress in tomato (Solanum lycopersicum L.)

Transcriptional and post-transcriptional regulation of RNAi-related gene expression during plant-virus interactions

A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants

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