RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus

Shi, Jianxin; Lang, Chunxiu; Wu, Xiao‐Tao; Liu, Renhu; Zheng, Tao; Zhang, Dongqing; Chen, Jinqing; Guan-ting, WU

doi:10.1016/j.bbrc.2015.09.062

Cited by 27 publications

(18 citation statements)

References 33 publications

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“…This is consistent with the results from studies on B. napus (Peng et al 2010;Shi et al 2015), C. abyssinica (Li et al 2015), A. thaliana (James et al 1995), and B. juncea (Kanrar et al 2006). The level of 18:3 was highly reduced from 40.4 to 2.6 %, which is likely resulting from the silencing of the FAD2 gene, and decreases were also seen in the levels of 18:2, 20:1, 20:2, 22:0, and 24:1, resulting in a total PUFA content of below 4 %.…”

Section: Discussionsupporting

confidence: 93%

“…The low PUFA content makes the oil more oxidation stable and more tolerant to high temperatures, thus being optimal as processing oil in the food industry or for the chemical industrial applications. Rapeseed oil with an oleic acid level over 60 % and an erucic acid level below 1 % is considered to be of high nutritional quality (Shi et al 2015), thus the seed oil composition achieved in transgenic lines of field cress in this study would have a better potential as a desirable oil in the food industry.…”

Section: Discussionmentioning

confidence: 98%

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Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

et al. 2016

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Simultaneous RNAi silencing of the FAD2 and FAE1 genes in the wild species Lepidium campestre improved the oil quality with 80 % oleic acid content compared to 11 % in wildtype. Field cress (Lepidium campestre) is a wild biennial species within the Brassicaceae family with desirable agronomic traits, thus being a good candidate for domestication into a new oilseed and catch crop. However, it has agronomic traits that need to be improved before it can become an economically viable species. One of such traits is the seed oil composition, which is not desirable either for food use or for industrial applications. In this study, we have, through metabolic engineering, altered the seed oil composition in field cress into a premium oil for food processing, industrial, or chemical industrial applications. Through seed-specific RNAi silencing of the field cress fatty acid desaturase 2 (FAD2) and fatty acid elongase 1 (FAE1) genes, we have obtained transgenic lines with an oleic acid content increased from 11 % in the wildtype to over 80 %. Moreover, the oxidatively unstable linolenic acid was decreased from 40.4 to 2.6 %, and the unhealthy erucic acid was reduced from 20.3 to 0.1 %. The high oleic acid trait has been kept stable for three generations. This shows the possibility to use field cress as a platform for genetic engineering of oil compositions tailor-made for its end uses.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 98%

Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

et al. 2016

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“…The RT-PCR analysis revealed that the levels of BnFAE1 were highly reduced in BnFAE1-Ri lines compared with the CY2 cultivar. Knockdown of BnFAE1 sharply reduced the levels of erucic acid (less than 3%), highly increased the contents of oleic acid (more than 60%), and slowly increased the polyunsaturated chain fatty acids (Shi et al, 2015).…”

Section: Enhancing Nutritional Valuesmentioning

confidence: 97%

The application of antisense technology for crop improvement: A review

Tilahun

Bezie

Kerisew

2021

Cogent Food & Agriculture

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The global population is increasing alarmingly, which calls for efficient methods of food production. Since domestication, people have been working on improving food crops for production and productivity. Genetic modification is one of the methods under use. Antisense technology, which includes antisense RNA (asRNA) and RNA interference (RNAi), is a gene regulatory strategy that is widely used for crop improvement. asRNA and RNAi mediated gene silencing is effective methods for regulating undesirable organic phenomena with no genomic alteration of the target gene by base pairing between asRNA and mRNA targets. Crops modified by engineering novel RNA interference and asRNA pathways allow small RNA molecules to alter organic phenomenon. Antisense technology plays an important role in generating high yielding, disease and pest resistant, high nutritional value, and stress-tolerant crop varieties. This review encompasses research articles on the two important antisense technologies to which RNAi (93%) was frequently used for crop improvement as compared to the asRNA (7%) technology. Regarding the crops improved using antisense technology, vegetables (41%) took the first attention. Most of the research articles reviewed were on improving crops for biotic stress resistance (29.6%), followed by fruit improvement and enhancing ABOUT THE AUTHOR Tadesse Tilahun, the first author, was born in 1988 in Ethiopia. He received a Bachelor of Sciences (BSc) degree in Applied Biology from

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“…FAD catalyzes the biosynthesis of polyunsaturated fatty acids, such as linoleic and linolenic acid [30,31], while FAE catalyzes the chain elongation reaction, resulting in the formation of long-chain fatty acids, including eicosenoic and erucic acid [30,32]. Previous studies have suggested that inhibition of FAE1 expression increases oleic acid and reduces erucic acid content in rapeseed seed oil [33,34], as did inhibition the expression of the BnFAD2 gene in transgenic seeds [34]. Jung et al [35] found that expression of the B. rapa BrFAD2 gene in an antisense orientation increased the synthesis of oleic acid in B. napus.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

Sun

Xue

et al. 2018

Agronomy

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Rapeseed (Brassica napus L.) with substantial lipid and oleic acid content is of great interest to rapeseed breeders. Overexpression of Glycine max transcription factors Dof4 and Dof11 increased lipid accumulation in Arabidopsis and microalgae, in addition to modifying the quantity of certain fatty acid components. Here, we report the involvement of GmDof4 and GmDof11 in regulating fatty acid composition in rapeseeds. Overexpression of GmDof4 and GmDof11 in rapeseed increased oleic acid content and reduced linoleic acid and linolenic acid. Both qPCR and the yeast one-hybrid assay indicated that GmDof4 activated the expression of FAB2 by directly binding to the cis-DNA element on its promoters, while GmDof11 directly inhibited the expression of FAD2. Thus, GmDof4 and GmDof11 might modify the oleic acid content in rapeseed by directly regulating the genes that are associated with fatty acid biosynthesis.

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RNAi knockdown of fatty acid elongase1 alters fatty acid composition in Brassica napus

Cited by 27 publications

References 33 publications

Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

The application of antisense technology for crop improvement: A review

Overexpression of Soybean Transcription Factors GmDof4 and GmDof11 Significantly Increase the Oleic Acid Content in Seed of Brassica napus L.

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