Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

Ivarson, Emelie; Ahlman, Annelie; Lager, Ida; Zhu, Lu

doi:10.1007/s00299-016-2016-9

Cited by 14 publications

(15 citation statements)

References 39 publications

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“…For example, the silencing of different genes (namely LABA1 and RAE2) via RNA interference has been used to shorten the awns of a rice introgression with long barber awns (Hua et al, 2015;Bessho-Uehara et al, 2016). Similarly, the silencing of genes of fatty acid biosynthesis (FAD2 and FAE1) improved the seed oil quality of the wild plant field cress (Lepidum campestre) (Ivarson et al, 2016). Mimicry of domestication has additionally been achieved by random mutagenesis followed by sequence evaluation of the ''targeted'' domestication gene.…”

Section: De Novo Domesticationmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

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Current global agricultural production must feed over 7 billion people. However, productivity varies greatly across the globe and is under threat from both increased competitions for land and climate change and associated environmental deterioration. Moreover, the increase in human population size and dietary changes are putting an ever greater burden on agriculture. The majority of this burden is met by the cultivation of a very small number of species, largely in locations that differ from their origin of domestication. Recent technological advances have raised the possibility of de novo domestication of wild plants as a viable solution for designing ideal crops while maintaining food security and a more sustainable lowinput agriculture. Here we discuss how the discovery of multiple key domestication genes alongside the development of technologies for accurate manipulation of several target genes simultaneously renders de novo domestication a route toward crops for the future.

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Section: De Novo Domesticationmentioning

confidence: 99%

De Novo Domestication: An Alternative Route toward New Crops for the Future

2019

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“…Much of the natural variation in seed oil composition in Arabidopsis has been attributed to FAD2 and FAE1 [ 74 ]. Indeed, downregulation of FAD2 and FAE1 in field cress also results in altered oil composition, drastically increasing the proportion of desirable oleic acid in the oil [ 75 ]. All the five genes shared high homology to the Arabidopsis orthologs and nonsynonymous polymorphisms were observed in FAD2 , KCS8 and WRI1 .…”

Section: Discussionmentioning

confidence: 99%

Identification of genes regulating traits targeted for domestication of field cress (Lepidium campestre) as a biennial and perennial oilseed crop

Gustafsson¹,

Willforss²,

Lopes-Pinto³

et al. 2018

BMC Genet

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BackgroundThe changing climate and the desire to use renewable oil sources necessitate the development of new oilseed crops. Field cress (Lepidium campestre) is a species in the Brassicaceae family that has been targeted for domestication not only as an oilseed crop that produces seeds with a desirable industrial oil quality but also as a cover/catch crop that provides valuable ecosystem services. Lepidium is closely related to Arabidopsis and display significant proportions of syntenic regions in their genomes. Arabidopsis genes are among the most characterized genes in the plant kingdom and, hence, comparative genomics of Lepidium-Arabidopsis would facilitate the identification of Lepidium candidate genes regulating various desirable traits.ResultsHomologues of 30 genes known to regulate vernalization, flowering time, pod shattering, oil content and quality in Arabidopsis were identified and partially characterized in Lepidium. Alignments of sequences representing field cress and two of its closely related perennial relatives: L. heterophyllum and L. hirtum revealed 243 polymorphic sites across the partial sequences of the 30 genes, of which 95 were within the predicted coding regions and 40 led to a change in amino acids of the target proteins. Within field cress, 34 polymorphic sites including nine non-synonymous substitutions were identified. The phylogenetic analysis of the data revealed that field cress is more closely related to L. heterophyllum than to L. hirtum.ConclusionsThere is significant variation within and among Lepidium species within partial sequences of the 30 genes known to regulate traits targeted in the present study. The variation within these genes are potentially useful to speed-up the process of domesticating field cress as future oil crop. The phylogenetic relationship between the Lepidium species revealed in this study does not only shed some light on Lepidium genome evolution but also provides important information to develop efficient schemes for interspecific hybridization between different Lepidium species as part of the domestication efforts.Electronic supplementary materialThe online version of this article (10.1186/s12863-018-0624-9) contains supplementary material, which is available to authorized users.

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“…The primer pairs for the AtWRI1, AtHb2 , and BvHb2 genes (Supplementary Table S1) were chosen among several pairs tested for their efficiency and specificity. The TIP41-like reference gene (Supplementary Table S1) was used as a reliable reference in this study as reported by Lenser and Theissen (2013), Muhlhausen et al (2013), and Ivarson et al (2016). For each line, three biological replicates and three technical replicates were conducted.…”

Section: Methodsmentioning

confidence: 99%

“…The oxidative and temperature stable oleic acid was increased from around 10% in the wild type (WT) to over 80% in the transgenic lines. Furthermore, the level of erucic acid was decreased from over 20% down to 0.2% (Ivarson et al, 2016). …”

Section: Introductionmentioning

confidence: 99%

Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

et al. 2017

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The wild species field cress (Lepidium campestre), belonging to the Brassicaceae family, has potential to be developed into a novel oilseed- and catch crop, however, the species needs to be further improved regarding some important agronomic traits. One of them is its low oil content which needs to be increased. As far as we know there is no study aiming at increasing the oil content that has been reported in this species. In order to investigate the possibility to increase the seed oil content in field cress, we have tried to introduce the Arabidopsis WRINKLED1 (AtWRI1) or hemoglobin (Hb) genes from either Arabidopsis thaliana (AtHb2) or Beta vulgaris (BvHb2) into field cress with the seed specific expression. The hypothesis was that the oil content would be increased by overexpressing these target genes. The results showed that the oil content was indeed increased by up to 29.9, 20.2, and 25.9% in the transgenic lines expressing AtWRI1, AtHb2, and BvHb2, respectively. The seed oil composition of the transgenic lines did not significantly deviate from the seed oil composition of the wild type plants. Our results indicate that genetic modification can be used in this wild species for its fast domestication into a future economically viable oilseed and catch crop.

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Significant increase of oleic acid level in the wild species Lepidium campestre through direct gene silencing

Cited by 14 publications

References 39 publications

De Novo Domestication: An Alternative Route toward New Crops for the Future

De Novo Domestication: An Alternative Route toward New Crops for the Future

Identification of genes regulating traits targeted for domestication of field cress (Lepidium campestre) as a biennial and perennial oilseed crop

Effects of Overexpression of WRI1 and Hemoglobin Genes on the Seed Oil Content of Lepidium campestre

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