RNAi-mediated knockdown of the mandibular organ-inhibiting hormone (MOIH) gene stimulates vitellogenesis in the Chinese mitten crab Eriocheir sinensis

Ding, Shuquan; Huang, Mengting; Sheng, Na; Chen, Tiantian; Xu, Ruijuan; Luo, Zhaoyuan; Huang, Xiuqin; Wan, Zhicheng; Su, Shiping; Li, Xilei

doi:10.1007/s12562-023-01680-y

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2024

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Cited by 3 publications

References 48 publications

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Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Li,

Cui,

Mao

et al. 2024

Aquaculture Reports

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Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Li,

Cui,

Mao

et al. 2024

Aquaculture Reports

View full text Add to dashboard Cite

Molecular Characterization and Functional Identification of the Insulin-Like Peptides Receptor Gene in the Oriental River Prawn Macrobrachium Nipponense

Li,

Cui,

et al. 2024

Preprint

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Silencing Multiple Crustacean Hyperglycaemic Hormone-Encoding Genes in the Redclaw Crayfish Cherax quadricarinatus Induces Faster Molt Rates with Anomalies

Black,

Banks,

Glendinning

et al. 2024

IJMS

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RNA interference (RNAi)-based biotechnology has been previously implemented in decapod crustaceans. Unlike traditional RNAi methodologies that investigate single gene silencing, we employed a multigene silencing approach in decapods based on chimeric double-stranded RNA (dsRNA) molecules coined ‘gene blocks’. Two dsRNA constructs, each targeting three genes of the crustacean hyperglycaemic hormone (CHH) superfamily of neuropeptides, were produced: Type II construct targeting Cq-Molt-inhibiting hormone 1 (MIH1), Cq-MIH-like 1 (MIHL1), and Cq-MIHL2 isoforms and Type I construct targeting Cq-ion transport peptide (Cq-ITP; a putative hybrid of CHH and MIH) and Cq-CHH and Cq-CHH-like (CHHL) isoforms. Both constructs were injected into juvenile redclaw crayfish, Cherax quadricarinatus, to determine the effects of multigene knockdown on molting and developmental processes. A 20-Hydroxyecdysone (20E) enzyme-linked immunosorbent assay (ELISA) and glucose assay were used to determine the effects of RNAi on molting and hemolymph glycemic activities, respectively. Multigene silencing reduced the intermolt interval by 23%. Statistically significant elevated 20E was recorded in treated intermolt individuals, consistent with the reduced intermolt interval as well as unique and abnormal phenotypes related to the molting process, which indicates a shift in 20E-induced cascade. There was no effect of RNAi treatment on hemolymph glucose level or molt increment. Through multigene silencing and subsequent annotation of gene networks, gene blocks may provide a tailored approach to investigate complex polygenic traits with RNAi in a more efficient and scalable manner.

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RNAi-mediated knockdown of the mandibular organ-inhibiting hormone (MOIH) gene stimulates vitellogenesis in the Chinese mitten crab Eriocheir sinensis

Cited by 3 publications

References 48 publications

Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Molecular characterization and functional identification of the insulin-like peptides receptor gene in the oriental river prawn Macrobrachium nipponense

Molecular Characterization and Functional Identification of the Insulin-Like Peptides Receptor Gene in the Oriental River Prawn Macrobrachium Nipponense

Silencing Multiple Crustacean Hyperglycaemic Hormone-Encoding Genes in the Redclaw Crayfish Cherax quadricarinatus Induces Faster Molt Rates with Anomalies

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