RNAi suppression of the ryanodine receptor gene results in decreased susceptibility to chlorantraniliprole in Colorado potato beetle Leptinotarsa decemlineata

Ryanodine receptor genes of the rice stem borer, Chilo suppressalis: Molecular cloning, alternative splicing and expression profiling

“…The CsRyR mRNA was successfully detected in all the test tissues, body part, developmental stages, and appeared abundantly in the head. This result was consistent with the body regions in the other insects, such as S. exigua [44], H. armigera [42], Leptinotarsa decemlineata [31], Pieris rapae [45], Bactrocera dorsalis [32], Plutella xylostella [46], and S. furcifera [47]. The results imply that the RyR is mainly distributed between the junctions of the muscular and neuron systems with regulatory function.…”

“…Due to difficulties in separation of the brain and muscle of C. suppressalis head, and the importance of RyR expression profile in other M A N U S C R I P T ACCEPTED MANUSCRIPT 4 insects [14,31,32], the whole head, including brain and muscle, was chosen for the detection of CsRyR mRNA. The qRT-PCR was performed on the ABI 7500 system (Applied Biosystems, CA, USA) in 20 μL reactions containing 1 μL cDNA, 10 μL SYBR ® Premix Ex Taq (Perfect Real Time) (Takara Co., Otsu, Japan), 0.4 μL forward GSP (qCsRyR-F and G3PDH-1-F) and reverse GSP (qCsRyR-R and G3PDH-1-R) (10 μM), 0.4 μL ROX Reference dye II and 7.8 μL ddH 2 O.…”

RNA interference: Applications and advances in insect toxicology and insect pest management

“…RyRs are a distinct class of intracellular calcium release channels that regulate entry of calcium into the cytosol from intracellular organelles. Several studies have shown that RNAi-mediated silencing of RyR genes can lead to decreased susceptibility of Leptinotarsa decemlineata [19] and Sogatella furcifera [20] to chlorantraniliprole, suggesting that RyRs are target molecules for chlorantraniliprole.…”

Instar-dependent systemic RNA interference response in Leptinotarsa decemlineata larvae

“…In contrast, 96% individuals show the RNAi phenotype when dsRNA is introduced by intra-abdominal injection in newly emerged bees [16]. In agricultural pests, bacterially expressed dsRNAs have been used to feed neonates [17,18], the second instars [19][20][21], the third instars [17,22], the fourth instars [23], the fifth instars [24], and the adults [25][26][27][28][29] of Lepidopteran and Coleopteran insect species. However, these studies are limited in specific genes and at particular life stages.…”

“…RNAi by dietary introduction of bacterially expressed dsRNAs is able to effectively knock down several target genes in L. decemlineata [19,21,25,[28][29][30][31][32][33]. Moreover, almost all genes involved in the RNAi pathway are expressed in the gut [34].…”