Rnapro•SAL: A Device for Rapid and Standardized Collection of Saliva RNA and Proteins

Chiang, Samantha H.; Thomas, Gerald A.; Liao, Wei; Grogan, Tristan; Buck, Robert L.; Fuentes, Laurel; Yakob, Maha; Laughlin, Mary J.; Schafer, Chris; Nazmul-Hossain, Abu N M; Fang, Wei; Elashoff, David; Slowey, Paul D.; Wong, David T.

doi:10.2144/000114254

Cited by 33 publications

(26 citation statements)

References 15 publications

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“…In this study, we evaluated the following collection methods; the classical passive drooling method (unstimulated whole saliva) and standardized tools for saliva collection (Pure·SAL™, and RNAPro·SAL™) from Oasis Diagnostics ® Corporation (Vancouver WA, USA) [ Figure 1 ]. [ 20 ] Subjects were asked to refrain from drinking, eating, or any oral hygiene procedure 2 h before sampling as reported by Topkas et al . [ 21 ] Before collection, subjects have been invited to rinse the mouth with drinking water for 15 s to remove any food debris, microorganisms, and desquamated epithelial cells.…”

Section: Methodsmentioning

confidence: 99%

Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method

et al. 2017

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Objective:The aim of the current study was to carry out a preliminary validation of devices for standardized collection of whole mouth fluid (WMF) in comparison to the passive drooling method for protein analysis in healthy subjects.Materials and Methods:A carefully designed sample collection/pretreatment protocol is crucial to the success of any saliva proteomics project. In this study, WMF was collected from healthy volunteers (n = 10, ages: 18–26 years). Individuals with any oral disease were excluded from the study group. In our study, we evaluated the following collection methods; the classical passive drooling method (unstimulated whole saliva) and standardized tools for saliva collection (Pure·SAL™, and RNAPro·SAL™) from Oasis Diagnostics® Corporation (Vancouver WA, USA). For estimation of protein levels, we used the bicinchoninic acid assay and protein assay kit (Thermo Fisher). The two-dimensional gel electrophoresis sample analysis was carried out for the estimation of proteins in one of the samples.Results:When gels were compared, the difference was seen in the resolution of spots. Protein spots were fading from high- to low-molecular weight masses. Hence, advanced devices in comparison to spitting method resulted in much clearer protein spots which in turn prove the validation of devices.Conclusions:In this study, we concluded that protein extraction could be possible by both methods such as passive drooling method and through advanced saliva collection devices (Pure·SAL™ and RNAPro·SAL™).

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Section: Methodsmentioning

confidence: 99%

Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method

et al. 2017

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“…Each kit typically yields 100 μg of DNA, ensuring adequate amounts for multiple genetic analyses 63 . Saliva can also be used for proteomic 29 , transcriptomic 64 and microbiome analysis 28,65 .…”

Section: Recommendations For Tissue Collection- Outlined In Tablementioning

confidence: 99%

Specimen Collection for Translational Studies in Hidradenitis Suppurativa

Byrd

Dina

Okoh

et al. 2019

Sci Rep

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Hidradenitis suppurativa (HS) is a chronic inflammatory disorder characterized by painful nodules, sinus tracts, and scars occurring predominantly in intertriginous regions. The prevalence of HS is currently 0.053–4%, with a predominance in African-American women and has been linked to low socioeconomic status. The majority of the reported literature is retrospective, population based, epidemiologic studies. In this regard, there is a need to establish a repository of biospecimens, which represent appropriate gender and racial demographics amongst HS patients. These efforts will diminish knowledge gaps in understanding the disease pathophysiology. Hence, we sought to outline a step-by-step protocol detailing how we established our HS biobank to facilitate the formation of other HS tissue banks. Equipping researchers with carefully detailed processes for collection of HS specimens would accelerate the accumulation of well-organized human biological material. Over time, the scientific community will have access to a broad range of HS tissue biospecimens, ultimately leading to more rigorous basic and translational research. Moreover, an improved understanding of the pathophysiology is necessary for the discovery of novel therapies for this debilitating disease. We aim to provide high impact translational research methodology for cutaneous biology research and foster multidisciplinary collaboration and advancement of our understanding of cutaneous diseases.

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“…Several methods can be applied for collection of saliva: (1) passive droll is the simplest approach but the saliva often has a high concentration of mucins and high viscosity and (2) the Salivette ® Systems [46][47][48], the Greiner Bio-One Saliva Kit, and the recently introduced RNA-Prosal [49]. All three sample collection systems have been used in the field, and publications describing their efficacy are available [50][51][52].…”

Section: Sample Collectionmentioning

confidence: 99%

Proteomics of the Salivary Fluid

Mitulović¹

2019

Salivary Glands - New Approaches in Diagnostics and Treatment

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Following the sequencing of the human genome, the mapping of the human proteome is the next task to being completed in order to gain knowledge on how proteins are involved in disease genesis, growth, therapy, and healing. As contrary to the genome, which is relatively static, the human proteome is significantly more complex and highly dynamic. Whilst the majority of the research is being focused on analyzing either the proteome of tumor tissues and tumor cells or the proteome of serum and plasma, little attention has been awarded to the analysis of proteomes in saliva or urine. The proteome in saliva can help providing important information on processes involving health issues in dentistry, head and neck cancers, gastric cancers or neurology, to name just a few. However, this is changing and the proteomics research community is increasingly focusing on deciphering the salivary proteome. So far, more than 3000 proteins have been identified in different studies and more is to come with new instrumentation and methods available. Some of the proteomics methods applied for analysis of salivary proteins will be discussed in this chapter.

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Rnapro•SAL: A Device for Rapid and Standardized Collection of Saliva RNA and Proteins

Cited by 33 publications

References 15 publications

Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method

Human salivary protein extraction from RNAPro·SAL™, Pure·SAL™, and passive drooling method

Specimen Collection for Translational Studies in Hidradenitis Suppurativa

Proteomics of the Salivary Fluid

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