This article investigates the problem of robust trajectory tracking for a marine surface vessel in the presence of asymmetrical error time-varying constraints and output feedback. To deal with system uncertainties, adaptive neural networks (NNs) are used to approximate unknown dynamics model parameters and external disturbances. To obtain unmeasured velocities, a predictive observer based on output feedback is developed to estimate unknown velocities. A tan-type asymmetric barrier Lyapunov function is used to deal with asymmetric error time-varying constraints. The high-frequency robust adaptive law is used to compensate for parameter estimation errors. The kinematic controller is designed based on the barrier Lyapunov function method, and the kinetics controller is designed base on an observer, an adaptive NN and a robust high-frequency control. All signals of the closed-loop systems are proved to be semiglobally uniformly and ultimately bounded via Lyapunov analysis, and the asymmetrical error constraints are not violated. Simulations are performed, and synthetic comparison is used to verify the feasibility and robustness of the proposed control law.