Rod-shaped and fluorine-substituted hydroxyapatite free of molecular immunopotentiators stimulates anti-cancer immunity in vivo

Wang, Xiupeng; Li, Xia; Ito, Atsuo; Watanabe, Yohei; Tsuji, Noriko M.

doi:10.1039/c6cc02848a

Cited by 38 publications

(15 citation statements)

References 32 publications

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“…It is well known that rod-shaped nano-HAP crystals grow in a calcium phosphate solution containing a small amount of fluoride 14, 15 . The challenge is to construct a nanorod array of oriented HAP on a substrate.…”

Section: Introductionmentioning

confidence: 99%

Artificial enamel induced by phase transformation of amorphous nanoparticles

Onuma

Iijima

2017

Sci Rep

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Human tooth enamel has tightly packed c-axis-oriented hydroxyapatite (HAP: Ca10(PO4)6(OH)2) nanorods with high elastic modulus. Fabrication of an enamel architecture in vitro supports the repair of teeth using HAP; however, existing methods require complex and laborious steps to form an enamel-like structure. Here we present a very simple and effective technique for forming artificial enamel in near-physiological solution using a substrate composed of amorphous calcium phosphate (ACP) nanoparticles. Without any functionalized modification of the substrate surface, faint dissolution and successive phase transformation automatically induce formation of an intermediate layer of low-crystalline HAP nanoparticles, on which highly oriented HAP nanorods grow by geometrical selection. We also show that an enamel structure forms on a substrate of amorphous calcium carbonate when the surface nanoparticles react so as to form an intermediate layer similar to that in ACP. Our results demonstrate that there is a wide range of substrate choices for nanorod array formation. Contrary to current understanding, a stable surface designed in nanoscale is not essential for the growth of arranged guest crystals. Reactive amorphous nanoparticles and their transformation efficiently induce a nanorod array structure.

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Section: Introductionmentioning

confidence: 99%

Artificial enamel induced by phase transformation of amorphous nanoparticles

Onuma

Iijima

2017

Sci Rep

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“…In previous reports, research demonstrated that the antitumor effect of HA NPs might be related to the intrinsic physicochemical characteristics, including crystal size, morphology, and surface functionality. − Many studies also attempted to change the structure of HA NPs in order to improve the inferior proliferation inhibition effect on tumor cells, but the improvement was very limited. Due to the initial preferential orientation of the HA crystal, , it was easier to obtain needle-like or rod-like HA nanocrystals with imbalanced aspect ratios in the process of growth. − The undiversified structure exerted poor adjustability as well as showed imperfect performance. Certainly, a few reports are also available on spherical HA NPs; however, the as-obtained structures are reported to have diameters up to several hundreds of nanometers with irregular assembled morphologies, − which cannot be defined as authentic sphere-like nanostructure.…”

mentioning

confidence: 99%

Antitumor Effect by Hydroxyapatite Nanospheres: Activation of Mitochondria-Dependent Apoptosis and Negative Regulation of Phosphatidylinositol-3-Kinase/Protein Kinase B Pathway

Zhao

Gao

et al. 2018

ACS Nano

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Hydroxyapatite nanoparticles (HA NPs) have been acknowledged for their benign biocompatibility and proliferation inhibition effect on tumor cells, attracting considerable attention for tumor therapeutics without late effects. However, unnoticeable tumor cytotoxicity of HA NPs limited the final clinical therapeutic efficacy. Herein, a two-phase synthetic approach was developed to synthesize sphere-like HA NPs by varying the conventional growth habit of HA precipitate. We present our in vitro and in vivo experimental evidence that spherical HA NPs have surprisingly high inhibitory activities against tumor cells. We demonstrate further, based on our experimental data, that the underlying cause for the death of the tumor cells is related to two concurrent pathways, the mitochondria-dependent apoptosis pathway and negative regulation of the phosphatidylinositol-3-kinase/protein kinase B (PIK3/AKT) pathway. The present study indicated that HA nanospheres can be engineered as nontoxic specific inhibitors for efficient tumor therapeutics with nanobiomaterials.

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“…For example, Cui and co‐workers [ 100 ] used carnosine modified graphene oxides as the adjuvant and carrier to deliver the antigen OVA to induce a strong OVA‐specific antibody response and recruit CD4+ T and CD8+ T cells. And, Tsuji and co‐workers [ 101 ] synthesized the fluorine‐substituted hydroxyapatite (FHA) nanoparticles, which are generated by F − instead of OH − in hydroxyapatite as both carrier and immune adjuvants for conveying OVA antigen to APCs ( Figure A). In this system, the uptake rate of FHA‐OVA by DCs was significantly enhanced.…”

Section: Intelligent Strategies Based On the Cells In The Immune Cycle For Improving Cancer Immunotherapymentioning

confidence: 99%

“…Reproduced with permission. [ 101 ] Copyright 2016, The Royal Society of Chemistry. B) Schematic diagram of bacterial membrane coated nanoparticle (BNP), which could adsorb the tumor antigens released in the process of radiotherapy to form a personalized vaccine.…”

Section: Intelligent Strategies Based On the Cells In The Immune Cycle For Improving Cancer Immunotherapymentioning

confidence: 99%

Immune Cycle‐Based Strategies for Cancer Immunotherapy

Liu

Zhang

et al. 2021

Adv Funct Materials

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The immune system is composed of immune organs, immune cells, and immunoactive substances, which plays a vital role in antitumor immunity in cancer immunotherapy. During the process of the antitumor immune response, many factors are involved in the cancer immune cycle. Therefore, developing intelligent strategies based on the steps of the cancer immune cycle to elicit the immune responses for enhanced cancer immunotherapy is of great significance. In this review, the key factors in each step of the cancer immune cycle are discussed, and then, the intelligent therapeutic strategies for modulating the immune surveillance against cancer are highlighted. Considering the demand for cancer immunotherapy in clinic, some suggestions for constructing new intelligent strategies are also put forward, which will make antitumor immunity more effective and advance the development of cancer immunotherapy.

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Rod-shaped and fluorine-substituted hydroxyapatite free of molecular immunopotentiators stimulates anti-cancer immunity in vivo

Cited by 38 publications

References 32 publications

Artificial enamel induced by phase transformation of amorphous nanoparticles

Artificial enamel induced by phase transformation of amorphous nanoparticles

Antitumor Effect by Hydroxyapatite Nanospheres: Activation of Mitochondria-Dependent Apoptosis and Negative Regulation of Phosphatidylinositol-3-Kinase/Protein Kinase B Pathway

Immune Cycle‐Based Strategies for Cancer Immunotherapy

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