1991
DOI: 10.1128/jb.173.14.4341-4346.1991
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Role and expression of the Bacillus subtilis rodC operon

Abstract: The role of the rodC operon in Bacillus subtilis was investigated. The operon encodes two genes (rodD and rodC) necessary for the synthesis of the cell wall teichoic acid. Transcription of this operon is responsive to levels of phosphate and to concentrations of magnesium ions in the growth medium. This regulation of mRNA production corresponds to conditions that dictate the type of polymer that will be synthesized for the cell wall, i.e., teichoic or teichuronic acid. While the introduction of multiple copies… Show more

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Cited by 15 publications
(12 citation statements)
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“…Under these conditions, the teichoic acid is replaced with teichuronic acid. The morphological changes associated with a defective teichoic acid resulting from a tagF (rodC) mutation were eliminated by the replacement of teichoic acid by teichuronic acid under these growth conditions (31). When KSS1571 was cultured under phosphate-limiting and phosphate-replete growth conditions, there were no differences seen in MreC-dependent growth, morphology, or polymeric material accumulation in the cells (data not shown).…”
Section: Fig 2 Growth and Mrec Expression By The Mrec-inducible Strmentioning
confidence: 91%
See 1 more Smart Citation
“…Under these conditions, the teichoic acid is replaced with teichuronic acid. The morphological changes associated with a defective teichoic acid resulting from a tagF (rodC) mutation were eliminated by the replacement of teichoic acid by teichuronic acid under these growth conditions (31). When KSS1571 was cultured under phosphate-limiting and phosphate-replete growth conditions, there were no differences seen in MreC-dependent growth, morphology, or polymeric material accumulation in the cells (data not shown).…”
Section: Fig 2 Growth and Mrec Expression By The Mrec-inducible Strmentioning
confidence: 91%
“…To induce a switch from teichoic acid to teichuronic acid synthesis, a lowphosphate medium was used as described previously (31).…”
Section: Methodsmentioning
confidence: 99%
“…1). tilis, the divIVA ORF was cloned under the control of the IPTG-inducible spac promoter in pTW880 (42). The resulting plasmid, pCHA2542 (Fig.…”
Section: Resultsmentioning
confidence: 99%
“…The PCR product was cloned in pT7Blue(R) (Novagen). The XbaI-SphI PCR product was subcloned into pTW880 (42). The resulting plasmid, pCHA2542, contains divIVA under the control of the IPTG (isopropyl-␤-Dthiogalactopyranoside)-inducible spac promoter.…”
Section: Methodsmentioning
confidence: 99%
“…Again, this may reflect differences in cell envelope architecture, since gram-positive rods appear to depend on an interaction between peptidoglycan and teichoic acids for stability of the cell envelope. Indeed, mutation at the rodC locus, which is involved in teichoic acid synthesis, also results in a spherical shape (21), and insertional inactivation of either of the genes in the rodC operon is lethal (42). Work on introducing less-drastic point mutations and in-frame deletions into these genes in order to clarify their function is currently under way.…”
Section: Discussionmentioning
confidence: 99%