Role and regulation of growth plate vascularization during coupling with osteogenesis in tibial dyschondroplasia of chickens

Abstract

Tibial dyschondroplasia (TD) is the most-prevalent leg disorder in fast-growing chickens; it is intractable and characterized by abnormal endochondral bone formation of proximal tibial growth-plates (TGPs). Previous studies have shown that bone is a highly vascularized tissue dependent on the coordinated coupling between angiogenesis and osteogenesis, but the underlying mechanisms of bone formation and bone remodeling are poorly defined in TD chickens. Here, we observed that inhibition of vasculogenesis and angiogenesis remarkably impaired vascular invasion in the hypertrophic chondrocyte zone of the TGPs, resulting in the massive death of chondrocytes due to a shortage of blood vessels and nutrients. Moreover, the balance of the OPG (osteoprotegerin)/RANKL (receptor activator of nuclear factor-kB ligand) system is also severely disrupted during the osteogenesis process while coupling with angiogenesis, both of which eventually lead to abnormal endochondral bone formation in TD chickens. Thus, the process of vascular formation in endochondral bone appears to initiate the pathological changes in TD, and improvement of this process during coupling with osteogenesis may be a potential therapeutic approach to treat this intractable disease.

Transcriptome-based screening of intracellular pathways and angiogenesis related genes at different stages of thiram induced tibial lesions in broiler chickens

“…KEGG pathways results of our study demonstrated that calcium signaling pathway was not enriched and differentially expressed on day 6, which was the clear indication of decreased or less Ca + ions transportation compared on day 2 and 15. The results of this study supported and added in the findings of Huang et al, [20], thiram significantly downregulated the expression of ATP2A3, which could be responsible for the reduction of Ca + 2 ions transportation similarly in TD chickens. Moreover, calcium signaling pathway shows that the chickens are being recovered from the deleterious effect of thiram on day 15.…”

“…Secondly, ATP2A3 was upregulated on day 2 and downregulated on day 15, which have been evaluated previously as a transporter of Ca + 2 ions [19]. A recent study reported that Ca + 2 ions decreased in TD chickens [20]. KEGG pathways results of our study demonstrated that calcium signaling pathway was not enriched and differentially expressed on day 6, which was the clear indication of decreased or less Ca + ions transportation compared on day 2 and 15.…”

Analysis of bone osteometry, mineralization, mechanical and histomorphometrical properties of tibiotarsus in broiler chickens demonstrates a influence of dietary chickpea seeds (Cicer arietinum L.) inclusion as a primary protein source

“…Bone growth depends also on the action of chondrocytes from the growth plate cartilage [44]. Recently, is has been shown that the growth plate cartilage of birds with induced dyschondroplasia is characterized by increased hypertrophic zone, and inhibited bone mass-related and bone structure-related parameters [45]. Chickens form the CPS group had heavier, longer and wider tibiotarsus than those of SBM group, with reduced hypertrophic zone and increased ossification zone and total growth plate thickness.…”

Abnormal Lipid Profile in Fast-Growing Broilers With Spontaneous Femoral Head Necrosis

“…Hematoxylin-eosin (HE) staining was performed to observe the degree of lesion changes. The area of lipid droplets in the liver and femoral head were analyzed using ImageJ ( Huang et al, 2018 ; Jahejo et al, 2020 ).…”

“…The bird had an obvious gait defect, which affected its ability to move about; Gait score of 4 (E) The bird had a severe gait defect; Gait score of 5 (F) The bird was incapable of sustained walking on its feet. the liver and femoral head were analyzed using ImageJ (Huang et al, 2018;Jahejo et al, 2020).…”