Role of Actin Cortex in the Subplasmalemmal Transport of Secretory Granules in PC-12 Cells

Lang, Thorsten; Wacker, Irene; Wunderlich, I.; Rohrbach, Alexander; Giese, Günter; Soldati, Thierry; Almers, Wolfhard

doi:10.1016/s0006-3495(00)76828-7

Cited by 199 publications

(231 citation statements)

References 45 publications

Supporting

Mentioning

199

Contrasting

Unclassified

Order By: Relevance

“…The second possibility is that dynamin-induced actin reorganization retards granule recruitment at a later stage of secretion. This is supported by the positive role of actin in vesicle transport (64)(65)(66)(67) and the pronounced defect in the second-phase secretion (Figure 3), which largely relies on new granule recruitment to the PM (8,11). To directly test this idea, we incubated β cells for 1 hour with 20 mM glucose and 10 nM glucagon-like peptide-1 (GLP-1), an incretin peptide that increases insulin secretion through cAMPand VAMP8-dependent mechanisms (68).…”

Section: And E)mentioning

confidence: 98%

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Fan¹,

Chen²,

Wu³

et al. 2015

Journal of Clinical Investigation

View full text Add to dashboard Cite

Section: And E)mentioning

confidence: 98%

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Fan¹,

Chen²,

Wu³

et al. 2015

Journal of Clinical Investigation

View full text Add to dashboard Cite

“…The values of N f are typically N/3 (N f ¼ 16) (74), N/2 (N f ¼ 28) (75), and 2N/3 (N f ¼ 50), where N is the number of total MSD data points (34). As a precise measure of the dynamic parameters requires a sufficient number of MSD points, we choose N f ¼ 40 (N/2, N ¼ 80 (w ¼ 1.6 s and Dt ¼ 20 ms)) in calculating the dynamics parameters of directed and confined diffusion.…”

Section: Msd Curve Fittingmentioning

confidence: 99%

Segmentation of 3D Trajectories Acquired by TSUNAMI Microscope: An Application to EGFR Trafficking

Liu

Perillo

Liu

et al. 2016

Biophysical Journal

View full text Add to dashboard Cite

Whereas important discoveries made by single-particle tracking have changed our view of the plasma membrane organization and motor protein dynamics in the past three decades, experimental studies of intracellular processes using singleparticle tracking are rather scarce because of the lack of three-dimensional (3D) tracking capacity. In this study we use a newly developed 3D single-particle tracking method termed TSUNAMI (Tracking of Single particles Using Nonlinear And Multiplexed Illumination) to investigate epidermal growth factor receptor (EGFR) trafficking dynamics in live cells at 16/43 nm (xy/z) spatial resolution, with track duration ranging from 2 to 10 min and vertical tracking depth up to tens of microns. To analyze the long 3D trajectories generated by the TSUNAMI microscope, we developed a trajectory analysis algorithm, which reaches 81% segment classification accuracy in control experiments (termed simulated movement experiments). When analyzing 95 EGF-stimulated EGFR trajectories acquired in live skin cancer cells, we find that these trajectories can be separated into three groups-immobilization (24.2%), membrane diffusion only (51.6%), and transport from membrane to cytoplasm (24.2%). When EGFRs are membrane-bound, they show an interchange of Brownian diffusion and confined diffusion. When EGFRs are internalized, transitions from confined diffusion to directed diffusion and from directed diffusion back to confined diffusion are clearly seen. This observation agrees well with the model of clathrin-mediated endocytosis.

show abstract

“…For example, in the neuronal PC12 cell line, myosin V was proposed to take over exocytic granules from microtubules and to ensure their anchoring 101 and short-range random movements 102 in the actin-rich cell cortex, thereby increasing the probability of membrane docking (FIG. 2).…”

Section: Outbound Trafficmentioning

confidence: 99%

Powering membrane traffic in endocytosis and recycling

Soldati

Schliwa

2006

Nat Rev Mol Cell Biol

Self Cite

314

279

View full text Add to dashboard Cite

| Early in evolution, the diversification of membrane-bound compartments that characterize eukaryotic cells was accompanied by the elaboration of molecular machineries that mediate intercompartmental communication and deliver materials to specific destinations. Molecular motors that move on tracks of actin filaments or microtubules mediate the movement of organelles and transport between compartments. The subjects of this review are the motors that power the transport steps along the endocytic and recycling pathways, their modes of attachment to cargo and their regulation.The emergence of eukaryotic cells was accompanied by the development of endomembrane systems that compartmentalize biochemical pathways and biosynthetic processes. An evolutionary trend towards increasing complexity and subcellular specialization necessitated the development of machineries for intercompartmental communication. Molecular assemblies evolved to confer specificity to the interactions of donor and acceptor compartments (budding, sorting, tethering and fusion). Cytoskeletal polymers such as actin filaments and microtubules, which help segregate genetic material and determine cell shape and subcellular architecture, were co-opted as tracks for transport. In animal cells, microtubules support long-range transport, whereas the more flexible actin filaments serve short-range movements both near the cell periphery and in the cell interior. Also, actin filaments can be woven into dense networks of short fibres through the action of crosslinkers and branchpromoting complexes. On the other hand, in many plant cells, bundled actin filaments can form extended tracks for long-distance transport. Owing to the gel-like nature of the cytoplasm, the Brownian movement of organelles is severely restricted and cargoes have to be transported to their destinations at the expense of energy. Furthermore, seemingly random, but motor-dependent, movement is proposed to increase the probability of vesicle collisions and therefore promote fusion events. Movement is powered by three ATP-dependent motors: myosin, kinesin and dynein. Over the course of eukaryotic evolution, these motors have diversified into a large number of families with specific tasks (FIG. 1).Here, we review the involvement of molecular motors in the movement of endosomes and in vesicular trafficking along the endocytic and recycling pathways. These pathways are summarized in FIG. 2. We do not, however,

show abstract

Role of Actin Cortex in the Subplasmalemmal Transport of Secretory Granules in PC-12 Cells

Cited by 199 publications

References 45 publications

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis

Segmentation of 3D Trajectories Acquired by TSUNAMI Microscope: An Application to EGFR Trafficking

Powering membrane traffic in endocytosis and recycling

Contact Info

Product

Resources

About