Role of Aquaporins in Spermatogenesis and Testicular Steroidogenesis

Kannan, A.; Panneerselvam, A.; Mariajoseph-Antony, Lezy Flora; Loganathan, Chithra; Prahalathan, Chidambaram

doi:10.1007/s00232-020-00114-5

Cited by 6 publications

(2 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In prior studies, Aqp0 was found to be localized to Sertoli cells; aqp1 exhibited expression in germ cells, particularly in sperm. Aqp7 and aqp8 were implicated in spermatid differentiation to sperm, while aqp11 was involved in cytoplasmic recycling and maintenance of Sertoli cells [9,[12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Wang

Zhao

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

The development and maturation of sperm entails intricate metabolic processes involving water molecules, amino acids, hormones, and various substances. Among these processes, the role of aquaporins (aqps) in the testis is crucial. Turbot (Scophthalmus maximus) is a significant marine flatfish species in China; however, natural egg laying in females is not feasible under cultured conditions. Consequently, artificial insemination becomes necessary, requiring the retrieval of sperm and eggs through artificial methods. In this study, we combined genomic, transcriptomics, RT-qPCR, computer-assisted sperm analysis (CASA), and immunohistochemistry to investigate the involvement of the aqp family in spermatogenesis in turbot. Through genomic data analysis, we identified 16 aqps genes dispersed across 13 chromosomes, each exhibiting the characteristic major intrinsic protein (MIP) domain associated with AQPs. The results from RNA-seq and RT-qPCR analysis revealed prominent expression of aqp4, 10, and 12 during the proliferative stage, whereas aqp1 showed primary expression during the mature stage. aqp11 displayed high expression levels during both MSII and MSV stages, potentially contributing significantly to the proliferation and maturation of male germ cells. Conversely, aqp8 showed elevated expression levels during the MSIII, MSIII-IV, and MSIV stages, suggesting its direct involvement in spermiogenesis. Immunohistochemical analysis unveiled the predominant localization of AQP1 protein in male germ cells rather than Sertoli cells, specifically concentrated in the head of sperm within cysts. Furthermore, a noteworthy decline in sperm motility was observed when sperm were subjected to treatment with either the AQP1-specific inhibitor (HgCl2) or the AQP1 antibody. However, no direct correlation was found between the expression of Smaqp1 and sperm quality. Overall, these findings provide new insights into the involvement of aqps in teleost spermatogenesis. Moreover, they hold potential for improving techniques related to sperm activation and cryopreservation, offering valuable knowledge for future advancements in this field.

show abstract

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Wang

Zhao

Wang

et al. 2023

IJMS

View full text Add to dashboard Cite

show abstract

“…The role AQPs in the field of reproduction has received increasing attention (14). We have found AQPs to be widely distributed in the cell membranes of various cells, and they can be divided into three subfamilies, based on different gene structures and functional properties of transport (15).…”

mentioning

confidence: 99%

Different expression and localization of aquaporin 7 and aquaporin 9 in granulosa cells, oocytes, and embryos of patients with polycystic ovary syndrome and the negatively correlated relationship with insulin regulation

Song

Wang

Hou

et al. 2021

Fertility and Sterility

View full text Add to dashboard Cite

Synthesis and biological evaluation of 3‐hydroxypyrazoles as aquaporin 9 inhibitors

Selvi,

Kannan,

Jayaraj

et al. 2024

Journal of Heterocyclic Chem

Self Cite

View full text Add to dashboard Cite

A series of 3‐hydroxypyrazole derivatives have been synthesized by a base‐promoted reaction of nitro‐substituted donor–acceptor cyclopropanes with hydrazines. The synthesized compounds have been investigated for their ability to inhibit aquaporin 9 (AQP9) in rat Leydig cells (LC‐540). The protein data bank structure for AQP9 was predicted using homology modeling; and the protein–ligand interaction for the synthesized hydroxyl pyrazole derivatives were analyzed using molecular modeling and docking studies. The results of in silico analyses showed that compound 5b had a higher binding affinity with AQP9 than other compounds. Further, in vitro studies conducted in LC‐540 cells confirmed that compound 5b effectively inhibits AQP9. Hence, compound 5b may be used as an inhibitor in enhancing our understanding of AQP9 function, and in the treatment of several diseases.

show abstract

Role of Aquaporins in Spermatogenesis and Testicular Steroidogenesis

Cited by 6 publications

References 54 publications

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Genome-Wide Identification of Aqp Family Related to Spermatogenesis in Turbot (Scophthalmus maximus)

Different expression and localization of aquaporin 7 and aquaporin 9 in granulosa cells, oocytes, and embryos of patients with polycystic ovary syndrome and the negatively correlated relationship with insulin regulation

Synthesis and biological evaluation of 3‐hydroxypyrazoles as aquaporin 9 inhibitors

Contact Info

Product

Resources

About