Role of arginine, a component of aqueous garlic extract, in remediation of sodium arsenite induced toxicity in A375 cells

Das, Bornita; Mandal, Samir; Chaudhuri, Keya

doi:10.1039/c3tx50098e

Cited by 9 publications

(6 citation statements)

References 21 publications

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“…Hemodialysis may be considered for patients who have renal failure. However, using A375 cells, the role of arginine, a component of aqueous garlic extract, has been shown in remediation of sodium arsenite induced toxicity [ 48 ]. In addition, coadministration of folic acid and vitamin B12 has been shown to prevent cardiac tissues from arsenic induced oxidative damage in vivo .…”

Section: Clinical and Pathophysiological Aspects Of Arsenic Toxicimentioning

confidence: 99%

Biomedical Implications of Heavy Metals Induced Imbalances in Redox Systems

Sharma

Singh

Siddiqi

2014

BioMed Research International

328

221

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Several workers have extensively worked out the metal induced toxicity and have reported the toxic and carcinogenic effects of metals in human and animals. It is well known that these metals play a crucial role in facilitating normal biological functions of cells as well. One of the major mechanisms associated with heavy metal toxicity has been attributed to generation of reactive oxygen and nitrogen species, which develops imbalance between the prooxidant elements and the antioxidants (reducing elements) in the body. In this process, a shift to the former is termed as oxidative stress. The oxidative stress mediated toxicity of heavy metals involves damage primarily to liver (hepatotoxicity), central nervous system (neurotoxicity), DNA (genotoxicity), and kidney (nephrotoxicity) in animals and humans. Heavy metals are reported to impact signaling cascade and associated factors leading to apoptosis. The present review illustrates an account of the current knowledge about the effects of heavy metals (mainly arsenic, lead, mercury, and cadmium) induced oxidative stress as well as the possible remedies of metal(s) toxicity through natural/synthetic antioxidants, which may render their effects by reducing the concentration of toxic metal(s). This paper primarily concerns the clinicopathological and biomedical implications of heavy metals induced oxidative stress and their toxicity management in mammals.

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Section: Clinical and Pathophysiological Aspects Of Arsenic Toxicimentioning

confidence: 99%

Biomedical Implications of Heavy Metals Induced Imbalances in Redox Systems

Sharma

Singh

Siddiqi

2014

BioMed Research International

328

221

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“…The DNA damage was quantified by alkaline single cell gel electrophoresis, also known as comet assay. [ 14 ] EAC cells were suspended in 0.5% (w/v) low melting agarose followed by layered over a frosted microscopic slide coated with a layer of 1% normal melting agarose. The slides were soaked in a lysing solution overnight at 4°C.…”

Section: Methodsmentioning

confidence: 99%

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Dolai

Islam²,

Haldar

2016

Indian J Pharmacol

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Objective:Anthocephalus cadamba (Roxb.) Miq. (Family: Rubiaceae), a folk medicine commonly known as “Kadam” in Bengali, has been used for the treatment of tumor. The methanolic extract of A. cadamba (MEAC) showing antitumor activity on Ehrlich ascites carcinoma (EAC) cells treated mice was already reported. This study was designed to study the apoptosis-inducing property of MEAC and its mechanism in EAC cells in mice.Materials and Methods:Apoptogenic morphology was determined by fluorescent DNA-binding double staining method using dyes acridine orange (AO)/ethidium bromide (EB). Comet assay was estimated to check the DNA damage. Flow cytometry (fluorescence-activated cell sorting [FACS]) was used to detect the apoptotic rate quantitatively by double labeling techniques using annexin V FITC/propidium iodide staining. Apoptotic protein expression was done using Western blotting assay method.Statistical Analysis:Results are expressed as mean ± standard deviation. Statistical analysis was performed using ANOVA followed by Dunnett's post hoc test of GraphPad Prism software. *P < 0.05, **P < 0.01 and ***P < 0.001 were considered statistically significant.Results:Apoptosis-inducing effect of MEAC on EAC cells was confirmed from AO/EB staining and FACS analysis. MEAC treatment showed dose-dependent induction of DNA damage. Apoptosis was induced by increasing the expression of multiple downstream factors such as pro-apoptotic protein p53 and p21 in EAC. Bax was up-regulated and anti-apoptotic protein Bcl-2 was down-regulated resulting in decrease of the Bcl-2/Bax ratio by MEAC treatment.Conclusion:Experimental results revealed that MEAC induces apoptosis by modulating the expression of some pro-apoptotic and anti-apoptotic proteins in EAC and thus exerts its anti-tumor activity.

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“…Normal value for urine is less than 50 mg/L and whole blood arsenic level is usually less than 1 mg/dL [ 39 ]. The treatment of arsenic intoxication is carried out mainly by chelation therapy using dimercaprol or succimer (2,3-dimercaptosuccinic acid, DMSA) and aqueous garlic extract [ 42 ]. However, some phytochemicals have been found to be very useful in combating arsenic toxicity as displayed in Table 1 and in the following section.…”

Section: Heavy Metals Influencing Cholinergic and Noncholinergic Smentioning

confidence: 99%

Phytochemicals Mediated Remediation of Neurotoxicity Induced by Heavy Metals

Gupta

Singh

Agrawal

et al. 2015

Biochemistry Research International

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Almost all the environmental components including both the abiotic and biotic factors have been consistently threatened by excessive contamination of heavy metals continuously released from various sources. Different heavy metals have been reported to generate adverse effects in many ways. Heavy metals induced neurotoxicity and impairment in signalling cascade leading to cell death (apoptosis) has been indicated by several workers. On one hand, these metals are required by the cellular systems to regulate various biological functions of normal cells, while on the other their biomagnification in the cellular systems produces adverse effects. The mechanism by which the heavy metals induce neurotoxicity follows free radicals production pathway(s) specially the generation of reactive oxygen species and reactive nitrogen species. These free radicals produced in excess have been shown to create an imbalance between the oxidative and antioxidative systems leading to emergence of oxidative stress, which may cause necrosis, DNA damage, and many neurodegenerative disorders. This mini review summarizes the current knowledge available on the protective role of varied natural products isolated from different herbs/plants in imparting protection against heavy metals (cadmium, lead, arsenic, and mercury) mediated neurotoxicity.

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Role of arginine, a component of aqueous garlic extract, in remediation of sodium arsenite induced toxicity in A375 cells

Cited by 9 publications

References 21 publications

Biomedical Implications of Heavy Metals Induced Imbalances in Redox Systems

Biomedical Implications of Heavy Metals Induced Imbalances in Redox Systems

Methanolic extract of Anthocephalus cadamba induces apoptosis in Ehrlich ascites carcinoma cells in experimental mice

Phytochemicals Mediated Remediation of Neurotoxicity Induced by Heavy Metals

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