Role of aromatase and androgen receptor expression in gonadal sex differentiation of ZW/ZZ-type frogs, Rana rugosa

Ohtani, Hiromi; Miura, Ikuo; Ichikawa, Youko

doi:10.1016/s1532-0456(02)00252-1

Cited by 46 publications

(42 citation statements)

References 24 publications

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“…These common origins are reflected in the similarity of both morphology and the sex-linked gene sequences between W and X, and Z and Y (Miura et al, 1998). Restriction fragment-length polymorphism analyses of AAT and AR discriminate between the W and Z chromosomes and between the X and Y chromosomes (Miura et al, 1998;Sakisaka et al, 2000;Ohtani et al, 2003). In the ZZ/ZW type, ZZ male and ZW female, respectively, are shown by homozygous bands a/a and heterozygous bands a/b, while in the XX/ XY type, XX female and XY male, respectively, are shown by bands b/b and b/a (Figure 2).…”

Section: Resultsmentioning

confidence: 99%

“…As for AR, we amplified the AR fragment using the above primers from ZZ and WW cDNA, sequenced and identified one nucleotide difference between them and found that it gives TaqI site to Z/Y alleles but not to W/X alleles. The sex linkage is confirmed using genomic southern hybridization (unpublished) and is partly proved by Ohtani et al (2003).…”

Section: Frogsmentioning

confidence: 87%

“…First strand cDNA was synthesized from 1 mg of the total RNA using dT 24 oligomer and reversetranscriptase (SuperScript II, BRL, New York, USA), basically according to the method described in Ogata et al (2003). For restriction fragment-length polymorphism analyses of sex-linked genes, AAT (ADP/ATP translocase, Miura et al, 1998) and AR (androgen receptor, Ohtani et al, 2003), they were amplified from the 1 ml of cDNA at 37 cycles of 94 1C 40 s, 62 1C 40 s, and 72 1C 30 s, ending with 72 1C 2 min in a 50 ml reaction solution containing 5 ml of 10 Â Ex buffer, 4 ml of 2.5 mM dNTP, 0.3 ml of Ex Taq (Takara, Tokyo, Japan) and the following primers: for AAT, forward 5 0 -CCTTGTG CTTCGTTTACCCAC-3 0 and reverse 5 0 -CCTGCTACCCT CATCTCTTGCA-3 0 , and for AR, forward 5 0 -GCGGAAA TGTTATGAGGCTGG-3 0 and reverse 5 0 -AAAATCCAC GCTCACCATCTG-3 0 .…”

Section: Frogsmentioning

confidence: 99%

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The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa

Ogata¹,

et al. 2007

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The Japanese frog, Rana rugosa, has two distinct sex chromosome types, XX/XY and ZZ/ZW. These two types are found in localized groups, separated geographically by a boundary area predicted to lie somewhere around Lake Biwa in central Japan. To determine this precise boundary, the heterogametic sex of 18 populations around Lake Biwa was examined by genotyping sex-linked genes. Phylogenetic relationships between the populations were also analyzed using mitochondrial 12S rRNA gene. Results showed that the Suzuka-Kii mountain range located east of Lake Biwa separated the XX/XY populations from the ZZ/ZW populations. Unexpectedly, from a phylogenetic perspective, the ZZ/ZW populations around Lake Biwa belonged not to the main ZW group but to the XY group. The authors propose that the ZZ/ZW populations around Lake Biwa diverged secondarily from the XX/XY group through a change of heterogametic sex, eventually forming a new group. This group was thus named the 'Neo-ZW group'. As the main ZW group inhabiting northwestern Japan is known to have a different male heterogametic origin, this finding shows that change of heterogametic sex from male to female may have occurred twice, and independently, during the frog speciation.

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Section: Resultsmentioning

confidence: 99%

Section: Frogsmentioning

confidence: 87%

Section: Frogsmentioning

confidence: 99%

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The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa

Ogata¹,

et al. 2007

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“…Although VTG is considered primarily synthesized in the liver, studies have shown that vtg mRNA could also be detected in extrahepatic tissues, especially the gonad, and could be induced following exposure to estrogenic chemicals (Wang et al, 2005). Dmrt1 plays critical role in male gonadal differentiation and maintenance (Ohtani et al, 2003;Matson et al, 2010) and it appears to be down-regulated in response to estrogen (Marchand et al, 2000) and up-regulated in response to androgen (Shibata et al, 2002). In the present study, vtg mRNA levels in liver and gonad and dmrt1 mRNA levels in gonad were also up-regulated in male fish upon 14-d exposure to PCP.…”

Section: Discussionmentioning

confidence: 99%

Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence

2017

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h i g h l i g h t s g r a p h i c a l a b s t r a c tThe effects of PCP on steroid system were studied by in silico and in vivo assays.In silico results indicated interacting potency with steroid receptors by PCP. PCP caused adverse effects on reproductive and interrenal system in rare minnow. The HPG/I axis of rare minnow showed different response at 14th and 28th day.a r t i c l e i n f o The present study investigated the effects on reproductive and interrenal system by pentachlorophenol (PCP) using in silico and in vivo assays. Molecular docking results indicated interacting potency of PCP with steroid receptors (ERa, ERb, AR, GR) but not Cytochrome P450 enzymes (CYPs). In the in vivo assay, sexually matured rare minnow (Gobiocypris rarus) was exposed to environmental relevant concentrations of PCP (0, 0.5, 5, 50 mg L À1 ). In male fish, 14-d exposure caused up-regulation of mRNA levels of hepatic era, erb, ar, gr, vtg and gonadal era, vtg, ar, dmrt1, providing evidence for agonistic activities for steroid receptors by PCP. The up-regulated mRNA of gnrh, crf, pomc in the brain also indicated feedforward responses of the hypothalamic-pituitary-gonadal/interrenal (HPG/I) axis. However, at 28th d the feed-forward response of the HPG axis seemed eased back and the HPI axis showed negative feedback responses. Corresponding changes including increases of plasma steroid hormones, inhibition of spermatogenesis, and decreased RSI were observed in male fish upon 28-d exposure to PCP. In the females, a transition from feed-forward responses to negative feedbacks of the HPG/I axis was also indicated by the transcriptional profiles at 14th and 28th day. Corresponding changes including increased E2, T and decreased C levels, degenerated ovaries, and decreased GSI and RSI were also observed. Overall, we concluded that PCP could interfere with steroid receptors, evoke responses of HPG/I axis, and finally result in adverse effects on reproductive and interrenal system in rare minnow at environmental relevant concentrations.

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“…Since then, a variety of expression studies have shown that aromatase is indeed a female specific factor that is primarily expressed in the ovaries of this species (Yoshida et al, 1996;Andrews et al, 1997). Amphibians are also strongly influenced by aromatase, and express this gene when exposed to female-inducing factors such as hormones and temperatures (Ohtani et al, 2003;Akatsuka et al, 2005). Furthermore, treatment with aromatase inhibitors has a masculinizing effect in urodeles, similar to the situation in chickens (Chardard and Dournon, 1999).…”

Section: Introductionmentioning

confidence: 93%

Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

Abramyan

Feng

Koopman

2009

Developmental Dynamics

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The development of the reproductive system in bufonids (true toads) is unique in several respects: sexual differentiation occurs later than in other anurans, and toads develop a Bidder's organ, a rudimentary ovary that can be manipulated in males to produce mature oocytes. To illuminate the genesis of this unusual reproductive system, we isolated from the cane toad (Bufo marinus) the orthologues of several known vertebrate sex-determining genes, determined their primary structure, and studied their expression by reverse transcriptase-polymerase chain reaction and in situ hybridization of tissue sections. We report here that cane toad Sox9, Dmrt1, and p450aromatase (Cyp19a1) are highly homologous to their counterparts in other vertebrates. They show profiles of expression that generally follow patterns observed in other taxa, but with some novel features. Our data suggest that these genes likely play key roles in sex determination and early gonad development in bufonids.

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Role of aromatase and androgen receptor expression in gonadal sex differentiation of ZW/ZZ-type frogs, Rana rugosa

Cited by 46 publications

References 24 publications

The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa

The ZZ/ZW sex-determining mechanism originated twice and independently during evolution of the frog, Rana rugosa

Pentachlorophenol affected both reproductive and interrenal systems: In silico and in vivo evidence

Cloning and expression of candidate sexual development genes in the cane toad (Bufo marinus)

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