Role of bacterial cell surface sulfhydryl sites in cadmium detoxification by Pseudomonas putida

Yu, Qiang; Mishra, Bhoopesh; Fein, Jeremy B.

doi:10.1016/j.jhazmat.2020.122209

Cited by 21 publications

(4 citation statements)

References 44 publications

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“…In the LB medium, the MIC of copper was 1.8 mM Cu 2+ , which was much higher than that in the basic medium (Figure 2 and Figure S2). Compared with the basic medium, the LB medium was richer in organic matter and inorganic salts, especially the sulfur‐contain amino acids, which facilitated bacteria to deal with heavy metals (Yu et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

“…At the same time, the production of H 2 S was significantly inhibited by the amending of Cu 2+ (Figure S4). It has been shown that the formation of copper sulfide (Kimber et al, 2020) or the compound with copper and glutathione (Stewart et al, 2020) could alleviate the biological toxicity of copper, while sulfhydryl groups combined with cations are important ways for Cu 2+ detoxification (Lin et al, 2020; Yu et al, 2020; Yu & Fein, 2016; Zeng et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Wang

Zhang

2023

Environ Microbiol Rep

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Resolving the heavy metal resistance mechanisms of microbes is crucial for understanding the bioremediation of the ecological environment. In this study, a multiple heavy metal resistance bacterium, Pseudoxanthomonas spadix ZSY‐33 was isolated and characterized. The copper resistance mechanism was revealed by analysis of the physiological traits, copper distribution, and genomic and transcriptomic data of strain ZSY‐33 cultured with different concentrations of copper. The growth inhibition assay in basic medium showed that the growth of strain ZSY‐33 was inhibited in the presence of 0.5 mM copper. The production of extracellular polymeric substances increased at a lower concentration of copper and decreased at a higher concentration of copper. Integrative analysis of genomic and transcriptomic, the copper resistance mechanism in strain ZSY‐33 was elucidated. At a lower concentration of copper, the Cus and Cop systems were responsible for the homeostasis of intracellular copper. As the concentration of copper increased, multiple metabolism pathways, including the metabolism of sulfur, amino acids, and pro‐energy were cooperated with the Cus and Cop systems to deal with copper stress. These results indicated a flexible copper resistance mechanism in strain ZSY‐33, which may acquire from the long‐term interaction with the living environment.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Wang

Zhang

2023

Environ Microbiol Rep

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“…Generally, sulfate metabolism is associated with bacterial resistance and immobilization of Cd (Zhu et al, 2017). Under Cd stress, bacteria assimilate more sulfate to produce thiol compounds, which are connected to the detoxi cation of Cd in the cell and the immobilization of Cd (Chen et al, 2016;Yu et al, 2020). Thus, increasing the activity of arylsulfatase in the BCM treatment indicated that the addition of biochar and AN-B15 improved the Cd resistance of the soil microbiome and Cd immobilization.…”

Section: Effects Of Strain An-b15 and Biochar On CD Speciation Ph And...mentioning

confidence: 99%

Bacteria-loaded biochar for the immobilization of cadmium in an alkaline-polluted soil

Yan,

Wang,

et al. 2023

Environ Sci Pollut Res

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The combination of biochar and bacteria is a promising strategy for the remediation of Cd-polluted soil.However, the synergistic mechanisms of biochar and bacteria for Cd immobilization remain unclear. In this study, the experiments were conducted to evaluate the effects of the combination of biochar and Pseudomonas sp. AN-B15, on Cd immobilization, soil enzyme activity, and soil microbiome. The results showed that biochar could directly reduce the motility of Cd by adsorption and formation of CdCO 3 precipitates, thereby protecting bacteria from Cd toxicity in the solution. Moreover, bacterial growth further induces the formation of CdCO 3 and CdS and enhances Cd adsorption by bacterial cells, leading to a higher Cd removal rate. Thus, bacterial inoculation signi cantly enhances Cd removal in the presence of biochar in the solution. Moreover, soil incubation experiments showed that bacteria-loaded biochar signi cantly reduced soil exchangeable Cd in comparison with other treatments by impacting soil microbiome. In particular, bacteria-loaded biochar increased the relative abundance of Bacillus, Lysobacter, and Pontibacter, causing an increase in pH, urease, and arylsulfatase, thereby passivizing soil exchangeable Cd and improving soil environmental quality in the natural alkaline Cd polluted soil. Overall, this study provides a systematic understanding of the synergistic mechanisms of biochar and bacteria for Cd immobilization in soil and new insights into the selection of functional strain for the e cient remediation of the contaminated environments by bacterial biochar composite.

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“…Loh and Chua [11] reported complete degradation of 600 mg/L benzoate in 11 h using P. putida ATCC 49451. Li et al [12] performed intergeneric protoplast fusion between P. putida and Bacillus subtilis to enhance benzoate tolerance as high as 10,000 mg/L with a specific growth rate of 30.9 × 10 -3 h. Yu et al [13] conducted the bacterial metal detoxification systems to observe the role of P. putida cells surface sulfhydryl sites in the detoxification of Cd. Their experimental results found that P. putida cells could detoxify Cd concentrations of 5 and 20 mg/L.…”

Section: Introductionmentioning

confidence: 99%

Kinetics of Cometabolic Transformation of 4-chlorophenol and Phenol Degradation by Pseudomonas putida Cells in Batch and Biofilm Reactors

Lin

2021

Processes

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The biodegradation kinetics of 4-chlorophenol (4-CP) and phenol and microbial growth of Pseudomonas putida (P. putida) cells were estimated in batch and biofilm reactors. The kinetic parameters of cells on phenol were determined using the Haldane formula. The maximum specific growth rate of P. putida on phenol, the half-saturation constant of phenol and the self-inhibition constant of phenol were 0.512 h−1, 78.38 mg/L and 228.5 mg/L, respectively. The yield growth of cells on phenol (YP) was 0.618 mg phenol/mg cell. The batch experimental results for the specific transformation rate of 4-CP by resting P. putida cells were fitted with Haldane kinetics to evaluate the maximum specific utilization rate of 4-CP, half-saturation constant of 4-CP, and self-inhibition constant of 4-CP, which were 0.246 h−1, 1.048 mg/L and 53.40 mg/L, respectively. The negative specific growth rates of cells on 4-CP obtained were fitted using a kinetic equation to investigate the true transformation capacity and first-order endogenous decay coefficient, which were 4.34 mg 4-CP/mg cell and 5.99 × 10−3 h−1, respectively. The competitive inhibition coefficients of phenol to 4-CP transformation and 4-CP to phenol degradation were 6.75 and 9.27 mg/L, respectively; therefore, phenol had a higher competitive inhibition of 4-CP transformation than the converse. The predicted model examining cometabolic transformation of 4-CP and phenol degradation showed good agreement with the experimental observations. The removal efficiencies for phenol and 4-CP were 94.56–98.45% and 96.09–98.85%, respectively, for steady-state performance.

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Role of bacterial cell surface sulfhydryl sites in cadmium detoxification by Pseudomonas putida

Cited by 21 publications

References 44 publications

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

The copper resistance mechanism in a newly isolated Pseudoxanthomonas spadixZSY‐33

Bacteria-loaded biochar for the immobilization of cadmium in an alkaline-polluted soil

Kinetics of Cometabolic Transformation of 4-chlorophenol and Phenol Degradation by Pseudomonas putida Cells in Batch and Biofilm Reactors

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