Role of bacterial products in periodontitis: humoral immune response to Eikenella corrodens

Johnson, David A.; Behling, Ulrich H.; Listgarten, Max A.; Nowotny, Alois

doi:10.1128/iai.22.2.382-386.1978

Cited by 27 publications

(13 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The data of others and our earlier publications (13,14), as well as those reported here, support our assumption that antibodies can provide a protective mechanism which may in part be the result of reduced mucosal penetrability of bacterial antigens. In an elegant study, Brandtzaeg and Tolo (3) showed that the excised mucosal membranes of previously immunized rabbits exhibited reduced transfer of the homologous antigen.…”

Section: Resultssupporting

confidence: 92%

“…We also reported that in the same monoinfected rat system the humoral immune response to the monoinfecting agent reduced the bone loss and immunosuppressive irradiation and accelerated the alveolar bone resorption (14).…”

mentioning

confidence: 64%

“…corrodens. The strain of E. corrodens used was CS10, which has been found to exist as two morphological variants, designated CS10A and CS1OB (14).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Humoral immunity and reduced periodontal bone loss in Eikenella corrodens-monoassociated rats

Behling¹,

Sallay²,

Sanavi³

et al. 1981

Infect Immun

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Resultssupporting

confidence: 92%

mentioning

confidence: 64%

See 1 more Smart Citation

Humoral immunity and reduced periodontal bone loss in Eikenella corrodens-monoassociated rats

Behling¹,

Sallay²,

Sanavi³

et al. 1981

Infect Immun

Self Cite

View full text Add to dashboard Cite

show abstract

“…A variety of immunoassays have been used to determine levels of systemic and/or local antibody to subgingival bacterial species in subjects with different forms of periodontal disease or periodontal health. These include immunofluorescence (32,38), hemag-glutination (10,24), radioimmunoassay (18,21,46,47), immunodiffusion in gels (42) and enzyme-linked immunosorbent assay (ELISA) (1, 10-17, 20, 23, 25, 28, 31, 36, 37, 40, 45, 51, 53, 54). ELISA has been the method of choice for numerous investigators due to its sensitivity and to its ability to evaluate antibody to a variety of antigens in large numbers of samples.…”

mentioning

confidence: 99%

Estimation of serum antibody to subgingival species using checkerboard immunoblotting

Sakellari

Socransky

Dibart

et al. 1997

Oral Microbiology and Immunology

View full text Add to dashboard Cite

Measurement of serum antibody to subgingival bacterial species has been useful in discriminating possible periodontal pathogens and in assessing the host's immune response to subgingival species. An immunoassay system was developed to measure the level of serum antibody to multiple subgingival species in multiple serum samples on a single nitrocellulose membrane. The principle steps of the assay are the following: 1) binding of antigens from bacterial preparations and protein A in parallel lanes on nitrocellulose membranes; 2) incubation of known concentrations of human immunoglobulin as well as various dilutions of serum from patients in lanes perpendicular to the antigen lanes; 3) incubation of the membrane with a peroxidase-conjugated second antibody; 4) detection of positive reactions by enhanced chemiluminescence. Emitted light was captured on a photographic film in which the positive reactions appeared as squares at the intersections of antigens with appropriate antibody. Antibody was quantified using a laser densitometer to compare the signal intensity of unknown samples with the ones generated by known amounts of human immunoglobulin captured on the same membrane. The assay permitted simultaneous screening and/or quantification of antibody in as many as 45 serum samples against up to 45 bacterial species. The mean and standard error of the coefficients of variation for replicates within an assay averaged 7.3 +/- 2.3%. Coefficients of variation of the assay run on different days for serum antibody to a range of subgingival species averaged 10.1 +/- 2.1%. Checkerboard immunoblotting is a simple and rapid immunoassay to permit quantification and/or screening of antibody to multiple subgingival species or antigens in multiple serum samples.

show abstract

“…This situation, according to Tanner and coworkers (24), may indicate that the etiological agent is highly virulent; E. (orrodens is recovered in high numbers relative to the other microorganisms in these sites and may thus be a major contributor to the disease process. The periodontopathic nature of E. corrodens in animal model systems has been firmly established by studies showing that oral colonization of E. (corrodens in gnotobiotic rats results in severe periodontal attachment loss within a matter of weeks (9,11).…”

mentioning

confidence: 99%

Biological activities of Eikenella corrodens outer membrane and lipopolysaccharide

Progulske¹,

Mishell²,

Trummel³

et al. 1984

Infect Immun

View full text Add to dashboard Cite

Highly purified preparations of the outer membrane and lipopolysaccharide (LPS) of Eikenella c orrodens strain ATCC 23834 and the outer membrane fraction (OMF) of strain 470 were tested in in vitro biological assays. The OMFs of both strains were found to be mitogenic for BDF and C3H/HeJ murine splenocytes. The E. c orrodens LPS was mitogenic for BDF spleen cells; however, doses of LPS as high as 50 pig/ml failed to stimulate C3H/HeJ cells. When incubated with T-lymphocyte-depleted C3H/HeJ splenocytes, the strain 23834 OMF demonstrated significant mitogenic activity, indicating that the OMF is a B-cell mitogen by a mechanism other than that elicited by conventional LPS. The E. (orrodens 23834 OMF and LPS were stimulators of bone resorption when tested in organ cultures of fetal rat long bones. In contrast, the strain 470 OMF was only weakly stimulatory. Both OMFs and LPSs demonstrated "endotoxic" activity, since as little as 0.062 p.g of E. (corrodens LPS and 0.015 .tg of the OMFs induced gelation in the Limttudls amebocyte clotting assay. Thus, despite having a "nonclassical"' LPS biochemistry, the E. (corrodens LPS elicits classical endotoxic activities. These results also indicate that the surface structures of E. (corrodens have significant biological activities as measured in vitro. The expression of such activities in vivo may play an important role in the pathogenesis of periodontitis as well as other E. corrodens infections.

show abstract

Role of bacterial products in periodontitis: humoral immune response to Eikenella corrodens

Cited by 27 publications

References 10 publications

Humoral immunity and reduced periodontal bone loss in Eikenella corrodens-monoassociated rats

Humoral immunity and reduced periodontal bone loss in Eikenella corrodens-monoassociated rats

Estimation of serum antibody to subgingival species using checkerboard immunoblotting

Biological activities of Eikenella corrodens outer membrane and lipopolysaccharide

Contact Info

Product

Resources

About