Role of biotransformation in the diazinon-induced toxicity in HepG2 cells and antioxidant protection by tetrahydrocurcumin

Miranda, Camila Araújo; Beretta, Eduardo Morais; Ferreira, Layra Araújo; Silva, Emmily Sousa da; Coimbra, Beatriz Zimermano; Pereira, Priscila Tartari; Miranda, Raúl; Dorta, Daniel Junqueira; Rodrigues, Flávia Thomaz Verechia; Mingatto, Fábio Ermínio

doi:10.1016/j.toxrep.2022.12.005

Cited by 7 publications

(4 citation statements)

References 60 publications

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“…The HepG2 is a cell line derived from a human hepatoblastoma, 26 and it has retained most of the metabolic functions of normal hepatocytes, 27 which is an extensively used cell line in assessing human health risks and in exploring the underlying molecular mechanisms 28 . MTT assay was conducted to determine the cytotoxicity of the blank micelles on the HepG2 cell.…”

Section: Methodsmentioning

confidence: 99%

Preparation and characterization of pH and thermally responsive perfluoropolyether acrylate copolymer micelles and investigation its drug‐loading properties

Lin

et al. 2023

J of Applied Polymer Sci

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In this study, pH and thermally responsive P(PFPHM 9 -b-DMAEMA-b-PEG-MEA 480 ) was prepared by atom transfer radical polymerization (ATRP) using perfluoropolyether hydroxyethyl methacrylate (PFPHM) as hydrophobic core, 2-(Dimethylamino) ethyl methacrylate as pH and thermal response function segment, and poly (ethylene glycol) methyl ether acrylate 480 as hydrophilic shell, respectively. The chemical structure, critical micelle concentration (CMC), lower critical solution temperature (LCST), biological toxicity, stability of micelles, drug-loading performance and cumulative drug release rate of prepared P(PFPHM 9 -b-DMAEMA-b-PEGMEA 480 ) were analyzed by 19 F NMR, 1 H NMR, FTIR, DLS, UV-Vis, laser confocal microscopy and fluorescence spectrophotometer. The results show that P(PFPHM 9 -b-DMAEMA-b-PEGMEA 480 ) exhibites a CMC of 7.5 g/L, LCST of 37.6 C and excellent micelle stability.Moreover, the analysis of drug-loading indicates that the polymer displayed drug loading efficiency (DL) is 21.3% and the encapsulation efficiency (EE) is 90.0%. Its cumulative drug release rates at pH = 6.5 (42 C) is almost double of those at pH = 7.4 (42 C), pH = 6.5 (37 C) and pH = 7.4 (37 C). These results suggeste that the P(PFPHM 9 -b-DMAEMA-b-PEGMEA 480 ) at pH ranging from 6.5 to 7.4 and temperature ranging from 37 to 42 C are pH and temperature sensitive. It has great potential application as a drug loading for DOX.amphiphilicity, drug-loading, lower critical solution temperature, perfluoropolyether acyl fluoride, pH and thermal responsiveness | INTRODUCTIONCancer has attracted widespread attention as one of the major threats to human health. Despite the significant progress made in current cancer treatment, a complete cure remains a thorny issue. 1 Chemotherapy is one of effective treatments for cancer, but the non-specific behavior of chemotherapy drugs can damage normal tissue cells, resulting in toxic side effects for patients. 2 Therefore, it is crucial to improve the utilization and selectivity of drugs on cancer cells. 3 Drug-loaded micelles formed by self-assembly of amphiphilic polymers as DDS have great potential for the treatment of cancer with good biocompatibility, high

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Section: Methodsmentioning

confidence: 99%

Preparation and characterization of pH and thermally responsive perfluoropolyether acrylate copolymer micelles and investigation its drug‐loading properties

Lin

et al. 2023

J of Applied Polymer Sci

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“…108,109 An in vitro study found that THC significantly mitigated cytotoxicity and reactive oxygen and nitrogen species generation in diazinoninduced HepG2 cells. 110 Therefore, THC might protect the liver from damage caused by drugs and toxins through its antioxidant effect.…”

Section: Food and Function Reviewmentioning

confidence: 99%

The role of tetrahydrocurcumin in disease prevention and treatment

Zhou,

Li,

Hua

et al. 2024

Food Funct.

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“…Several molecular targets of THC have been identified, including targets that make it effective against cancers such as anti-proliferative activity by cell cycle arrest and decreasing proliferative molecule (cyclin D1, PCNA), anti-oxidative activity by increasing radical scavenging activity and antioxidant enzyme (Miranda et al., 2023 ), inhibition of metastasis by decreasing invasion, migration, cell adhesion and angiogenesis, induction of programmed cell death by increasing apoptosis and autophagy and increase of immune function by increasing phagocytosis and NK cells activity (Lai et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Novel tetrahydrocurcumin integrated mucoadhesive nanocomposite κ-carrageenan/xanthan gum sponges: a strategy for effective local treatment of oral cancerous and precancerous lesions

Elbanna,

Ebada,

Abdallah

et al. 2023

Drug Delivery

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Oral cancer is one of the leading causes of death worldwide. Oral precancerous lesions (OPL) are the precursors of oral cancer, with varying degrees of progression. Tetrahydrocurcumin (THC) is a major metabolite of curcumin with superior anticancer properties against various types of cancer. However, THC’s clinical outcome is limited by its poor aqueous solubility. Herein, we developed novel mucoadhesive biopolymer-based composite sponges for buccal delivery of THC, exploiting nanotechnology and mucoadhesion for efficient prevention and treatment of oral cancer. Firstly, THC-nanocrystals (THC-NC) were formulated and characterized for subsequent loading into mucoadhesive composite sponges. The anticancer activity of THC-NC was assessed on a human tongue squamous carcinoma cell line (SCC-4). Finally, the chemopreventive activity of THC-NC loaded sponges (THC-NC-S) was examined in DMBA-induced hamster OPL. The selected THC-NC exhibited a particle size of 532.68 ± 13.20 nm and a zeta potential of −46.08 ± 1.12 mV. Moreover, THC-NC enhanced the anticancer effect against SCC-4 with an IC50 value of 80 µg/mL. THC-NC-S exhibited good mucoadhesion properties (0.24 ± 0.02 N) with sustained drug release, where 90% of THC was released over 4 days. Furthermore, THC-NC-S had a magnificent potential for maintaining high chemopreventive activity, as demonstrated by significant regression in the dysplasia degree and a decline in cyclin D1 (control: 40.4 ± 12.5, THC-NC-S: 12.07 ± 5.2), culminating in significant amelioration after 25 days of treatment. Conclusively, novel THC-NC-S represent a promising platform for local therapy of OPL, preventing their malignant transformation into cancer.

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Role of biotransformation in the diazinon-induced toxicity in HepG2 cells and antioxidant protection by tetrahydrocurcumin

Cited by 7 publications

References 60 publications

Preparation and characterization of pH and thermally responsive perfluoropolyether acrylate copolymer micelles and investigation its drug‐loading properties

Preparation and characterization of pH and thermally responsive perfluoropolyether acrylate copolymer micelles and investigation its drug‐loading properties

The role of tetrahydrocurcumin in disease prevention and treatment

Novel tetrahydrocurcumin integrated mucoadhesive nanocomposite κ-carrageenan/xanthan gum sponges: a strategy for effective local treatment of oral cancerous and precancerous lesions

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