Role of calmodulin in thermotolerance

Jia, Lixiu; Chu, Hongye; Wu, Dan; Mao, Feng; Zhao, Liqun

doi:10.4161/psb.28887

Cited by 12 publications

(7 citation statements)

References 12 publications

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“…Upstream events of the HSR include perception and signal transduction of HS. Jia et al [ 55 ] demonstrated that Hsf activity and Hsp production involved in thermotolerance are a result of cross-talk among H 2 O 2 , nitric oxide (NO), Ca 2+ channels, and calmodulin (CaM). In particular, CaM activates Hsfs, protein kinase, phosphatase, and cyclic nucleotide-gated ion channels.…”

Section: Resultsmentioning

confidence: 99%

Global Gene-Expression Analysis to Identify Differentially Expressed Genes Critical for the Heat Stress Response in Brassica rapa

Dong

Lee

et al. 2015

PLoS ONE

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Genome-wide dissection of the heat stress response (HSR) is necessary to overcome problems in crop production caused by global warming. To identify HSR genes, we profiled gene expression in two Chinese cabbage inbred lines with different thermotolerances, Chiifu and Kenshin. Many genes exhibited >2-fold changes in expression upon exposure to 0.5– 4 h at 45°C (high temperature, HT): 5.2% (2,142 genes) in Chiifu and 3.7% (1,535 genes) in Kenshin. The most enriched GO (Gene Ontology) items included ‘response to heat’, ‘response to reactive oxygen species (ROS)’, ‘response to temperature stimulus’, ‘response to abiotic stimulus’, and ‘MAPKKK cascade’. In both lines, the genes most highly induced by HT encoded small heat shock proteins (Hsps) and heat shock factor (Hsf)-like proteins such as HsfB2A (Bra029292), whereas high-molecular weight Hsps were constitutively expressed. Other upstream HSR components were also up-regulated: ROS-scavenging genes like glutathione peroxidase 2 (BrGPX2, Bra022853), protein kinases, and phosphatases. Among heat stress (HS) marker genes in Arabidopsis, only exportin 1A (XPO1A) (Bra008580, Bra006382) can be applied to B. rapa for basal thermotolerance (BT) and short-term acquired thermotolerance (SAT) gene. CYP707A3 (Bra025083, Bra021965), which is involved in the dehydration response in Arabidopsis, was associated with membrane leakage in both lines following HS. Although many transcription factors (TF) genes, including DREB2A (Bra005852), were involved in HS tolerance in both lines, Bra024224 (MYB41) and Bra021735 (a bZIP/AIR1 [Anthocyanin-Impaired-Response-1]) were specific to Kenshin. Several candidate TFs involved in thermotolerance were confirmed as HSR genes by real-time PCR, and these assignments were further supported by promoter analysis. Although some of our findings are similar to those obtained using other plant species, clear differences in Brassica rapa reveal a distinct HSR in this species. Our data could also provide a springboard for developing molecular markers of HS and for engineering HS tolerant B. rapa.

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Section: Resultsmentioning

confidence: 99%

Global Gene-Expression Analysis to Identify Differentially Expressed Genes Critical for the Heat Stress Response in Brassica rapa

Dong

Lee

et al. 2015

PLoS ONE

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“…We have also studied other physiological parameters, such as gs, E, Pn and Rd in the Wsamisgt lines after heat stress. In general, gs appeared to be controlled by CO 2 and water vapor which prevents leaf desiccation (Zhao et al 2014). After heat stress, gs was increased in Wsamisgt lines which increased the E. Furthermore, due to low ETR between PSI and PSII, Pn was also decreased in Wsamisgt lines.…”

Section: Discussionmentioning

confidence: 96%

“…A few members of HSPs are the molecular chaperons and essential in maintaining protein degradation, protein folding and activation of proteins involved in signal transduction and control of cell cycle in stress conditions (Wang et al , Zhang et al ). The expressions of HSP genes in the plants are governed by heat shock signaling pathway which is regulated by H 2 O 2 and other ROS, such as NO (Jia et al ). After heat stress, Wsamisgt lines enhanced expression of HSP encoding genes, such as WsHSP24 , WsHSP80 , WsHSP83 and WsHSP101 as compared to control plants (Fig.…”

Section: Discussionmentioning

confidence: 99%

Sterol glycosyltransferases required for adaptation of Withania somnifera at high temperature

Singh

Tiwari

Singh

et al. 2017

Physiologia Plantarum

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Heat is a major environmental stress factor that confines growth, productivity, and metabolism of plants. Plants respond to such unfavorable conditions through changes in their physiological, biochemical and developmental processes. Withania somnifera, an important medicinal plant, grows in hot and dry conditions, however, molecular mechanisms related to such adaptive properties are not known. Here, we elucidated that members of the sterol glycosyltransferases (SGT) gene family play important roles in the survival of W. somnifera under adverse conditions through maintaining the integrity of the membrane. SGTs are enzymes involved in sterol modifications and participate in metabolic flexibility during stress. Silencing of WsSGT members, for instance WsSGTL1, WsSGTL2 and WsSGTL4, was inimical for important physiological parameters, such as electron transport rate, photochemical quantum yield, acceptor side limitation, non-photochemical quenching (NPQ), Fv/Fm and net photosynthetic rate, whereas stomatal conductance, transpiration rate and dark respiration rates (Rds) were increased. Decreased NPQ and increased Rds helped to generate significant amount of ROS in the Wsamisgt lines. After heat stress, H O , lipid peroxidation and nitric oxide production increased in the Wsamisgt lines due to high ROS generation. The expression of HSPs in Wsamisgt lines might be involved in regulation of physiological processes during stress. We have also observed increased proline accumulation which might be involved in restricting water loss in the Wsamisgt lines. Taken together, our observations revealed that SGTL enzyme activity is required to maintain the internal damages of the cell against high temperature by maintaining the sterol vs sterol glycosides ratio in the membranes of W. somnifera.

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“…Calmodulin is an important protein; because it decodes Ca 2+ -dependent and-independent signals and could be helpful in in-depth understanding of the biological molecular mechanisms and signals. [ 19 20 21 22 ]…”

Section: Discussionmentioning

confidence: 99%

Analysis of common bean (Phaseolus vulgaris L., genotype BAT93) calmodulin cDNA using computational tools

Amelia¹,

Singh

Shah³

et al. 2015

Phcog Res

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Background:Common bean (Phaseolus vulgaris L.) is an important part of the human diet and serves as a source of natural products. Identification and understanding of genes in P. vulgaris is important for its improvement. Characterization of expressed sequence tags (ESTs) is one of the approaches in understanding the expressed genes. For the understanding of genes expression in P. vulgaris pod-tissue, research work of ESTs generation was initiated by constructing cDNA libraries using 5-day and 20-day old bean-pod-tissues. Altogether, 5972 cDNA clones were isolated to have ESTs. While processing ESTs, we found a transcript for calmodulin (CaM) gene. It is an important gene that encodes for a calcium-binding protein and known to express in all eukaryotic cells. Hence, this study was undertaken to analyse and annotate it.Objective:The objective of this study was to analyze and annotate P. vulgaris CaM (PvCaM) gene cDNA and its deduced protein (amino acids) sequence.Materials and Methods:Both strands of PvCaM cDNA clone were sequenced using M13 forward and reverse primer to elucidate the nucleotide sequence. The cDNA sequence and deduced protein sequence were analyzed and annotated using bioinformatics tools available online. The secondary structures and three-dimensional (3D) structure of PvCaM protein were predicted using the Phyre automatic fold recognition server.Results:Results showed that PvCaM cDNA is 818 bp in length. The cDNA analysis results showed that it contains an open reading frame that encodes for 149 amino acid residues. The deduced protein sequence analysis results showed the presence of conserved domains required for CaM function. The predicted secondary structures and 3D structure are analogous to the Solanum tuberosum CaM.Conclusions:This study analyzed and annotated PvCaM cDNA and protein. However, in order to obtain a complete understanding of PvCaM protein, further study on its expression, structure and regulation is essential.

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Role of calmodulin in thermotolerance

Cited by 12 publications

References 12 publications

Global Gene-Expression Analysis to Identify Differentially Expressed Genes Critical for the Heat Stress Response in Brassica rapa

Global Gene-Expression Analysis to Identify Differentially Expressed Genes Critical for the Heat Stress Response in Brassica rapa

Sterol glycosyltransferases required for adaptation of Withania somnifera at high temperature

Analysis of common bean (Phaseolus vulgaris L., genotype BAT93) calmodulin cDNA using computational tools

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