Role of Candida albicans Tem1 in mitotic exit and cytokinesis

Colletotrichum orbiculare Regulates Cell Cycle G1/S Progression via a Two-Component GAP and a GTPase to Establish Plant Infection

“…Our results suggest that C. orbiculare has plant pathogen-specific roles for the two-component GAP and its GTPase that are involved in functional development in various yeast and fungal species (Figure 10). In S. cerevisiae and C. albicans, Bub2p/Bfa1p negatively regulates GTPase Tem1p, thereby triggering the MEN pathway, which controls mitotic exit and cytokinesis via Ser/Thr kinase Cdc15p (Bardin and Amon, 2001;Finley et al, 2008;Milne et al, 2014). A similar cascade, the SIN, has been reported in S. pombe and A. nidulans, and its primary role appears to be in regulating septation rather than mitotic exit (Bruno et al, 2001;Krapp and Simanis, 2008;Kim et al, 2009).…”

“…In S. cerevisiae and C. albicans, the Bub2p/Bfa1p Rab GAP complex negatively regulates the direct downstream GTPase Tem1p, a component of MEN (Bardin and Amon, 2001;Milne et al, 2014). In S. pombe and A. nidulans, an analogous cascade SIN is conserved, and Cdc16/BUBA and Byr4/BYRA, homologs of Bub2p and Bfa1p, negatively regulate the downstream GTPase Tem1p homolog Spg1/ASGA (Furge et al, 1998;Li et al, 2000;Kim et al, 2006).…”

“…In the budding yeast Saccharomyces cerevisiae and the multimorphic fungus Candida albicans, exit from mitosis and entry into cytokinesis are regulated by the mitotic exit network (MEN), a GTPase-regulated kinase cascade (Bardin and Amon, 2001;Milne et al, 2014). The MEN consists of the central GTPase Tem1p, which is regulated by the two-component GTPaseactivating protein (GAP), consisting of Bub2p and Bfa1p.…”

Candida albicans Cdc15 is essential for mitotic exit and cytokinesis

“…A number of key constituents of the MEN have now been studied in C . albicans including Tem1, Dbf2 and Cdc14 35 – 37 . However, strains depleted of these components demonstrate a range of different phenotypes, suggesting that in C .…”

“…albicans this network is not a simple linear signalling cascade that operates through the release and activation of Cdc14 as a common end point. The GTPase Tem1 is essential, with cells depleted of it forming filaments and exhibiting a clear mitotic exit defect through arresting with an extended spindle 37 . In contrast, although the downstream kinase Dbf2 was also shown to be essential its primary function was seen to be in regulating spindle organisation and signalling for actomyosin ring contraction 36 .…”