Role of cytokinins in the regulation of stomatal conductance of wheat seedlings under conditions of rapidly changing local temperature

Веселова, С. В.; Фархутдинов, Р.Г.; Веселов, Д. С.; Kudoyarova, G. R.

doi:10.1134/s1021443706060057

Cited by 25 publications

(13 citation statements)

References 16 publications

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“…Transgenic Arabidopsis plants with stabilised high levels of CKs exhibited increased resistance against infection by the hemibiotrophic pathogen Verticillium longisporum [57]. CKs have also been shown to regulate stomatal conductance of wheat seedlings, pointing to the role of these hormonal molecules in plant-environment interactions [58]. Furthermore, the activation of (zeatin-type) CK signalling has been observed in Arabidopsis plants primed with a PGPR, B. subtilis [59].…”

Section: Resultsmentioning

confidence: 99%

Unravelling the Metabolic Reconfiguration of the Post-Challenge Primed State in Sorghum bicolor Responding to Colletotrichum sublineolum Infection

et al. 2019

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Priming is a natural phenomenon that pre-conditions plants for enhanced defence against a wide range of pathogens. It represents a complementary strategy, or sustainable alternative that can provide protection against disease. However, a comprehensive functional and mechanistic understanding of the various layers of priming events is still limited. A non-targeted metabolomics approach was used to investigate metabolic changes in plant growth-promoting rhizobacteria (PGPR)-primed Sorghum bicolor seedlings infected with the anthracnose-causing fungal pathogen, Colletotrichum sublineolum, with a focus on the post-challenge primed state phase. At the 4-leaf growth stage, the plants were treated with a strain of Paenibacillus alvei at 108 cfu mL−1. Following a 24 h PGPR application, the plants were inoculated with a C. sublineolum spore suspension (106 spores mL−1), and the infection monitored over time: 1, 3, 5, 7 and 9 days post-inoculation. Non-infected plants served as negative controls. Intracellular metabolites from both inoculated and non-inoculated plants were extracted with 80% methanol-water. The extracts were chromatographically and spectrometrically analysed on an ultra-high performance liquid chromatography (UHPLC) system coupled to high-definition mass spectrometry. The acquired multidimensional data were processed to create data matrices for chemometric modelling. The computed models indicated time-related metabolic perturbations that reflect primed responses to the fungal infection. Evaluation of orthogonal projection to latent structure-discriminant analysis (OPLS-DA) loading shared and unique structures (SUS)-plots uncovered the differential stronger defence responses against the fungal infection observed in primed plants. These involved enhanced levels of amino acids (tyrosine, tryptophan), phytohormones (jasmonic acid and salicylic acid conjugates, and zeatin), and defence-related components of the lipidome. Furthermore, other defence responses in both naïve and primed plants were characterised by a complex mobilisation of phenolic compounds and de novo biosynthesis of the flavones, apigenin and luteolin and the 3-deoxyanthocyanidin phytoalexins, apigeninidin and luteolinidin, as well as some related conjugates.

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Section: Resultsmentioning

confidence: 99%

Unravelling the Metabolic Reconfiguration of the Post-Challenge Primed State in Sorghum bicolor Responding to Colletotrichum sublineolum Infection

et al. 2019

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“…The cause of this diVerence may be either species speciWcity of response or diVerences in conditions of plant growing. Air warming has been shown to lead to accumulation of cytokinins in leaves and it was suggested that the eVect may contribute to keeping stomata in open state (Veselova et al 2006). However, the role of cytokinins for stomatal regulation is not well established (Dodd 2003).…”

Section: Discussionmentioning

confidence: 98%

Involvement of root ABA and hydraulic conductivity in the control of water relations in wheat plants exposed to increased evaporative demand

Kudoyarova

Веселова

Hartung

et al. 2010

Planta

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We studied the possible involvement of ABA in the control of water relations under conditions of increased evaporative demand. Warming the air by 3°C increased stomatal conductance and raised transpiration rates of hydroponically grown Triticum durum plants while bringing about a temporary loss of relative water content (RWC) and immediate cessation of leaf extension. However, both RWC and extension growth recovered within 30 min although transpiration remained high. The restoration of leaf hydration and growth were enabled by increased root hydraulic conductivity after increasing the air temperature. The use of mercuric chloride (an inhibitor of water channels) to interfere with the rise on root hydraulic conductivity hindered the restoration of extension growth. Air warming increased ABA content in roots and decreased it in shoots. We propose this redistribution of ABA in favour of the roots which increased the root hydraulic conductivity sufficiently to permit rapid recovery of shoot hydration and leaf elongation rates without the involvement of stomatal closure. This proposal is based on known ability of ABA to increase hydraulic conductivity confirmed in these experiments by measuring the effect of exogenous ABA on osmotically driven flow of xylem sap from the roots. Accumulation of root ABA was mainly the outcome of increased export from the shoots. When phloem transport in air-warmed plants was inhibited by cooling the shoot base this prevented ABA enrichment of the roots and favoured an accumulation of ABA in the shoot. As a consequence, stomata closed.

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“…CKs accelerate the regeneration and the de novo formation of chloroplasts by regulating membrane formation and the synthesis of components of the electron transport system (Sestak and Pospisilova, 1986;Chernyad'ev, 2000;Pospíšilová et al, 2000;Veselova et al, 2006). The increase in catalase inside peroxisomes, the physical association between chloroplasts, peroxisomes, and mitochondria, and the increase in the CO 2 compensation point implied the occurrence of photorespiration in the transgenic plants.…”

Section: Discussionmentioning

confidence: 99%

Cytokinin-Dependent Photorespiration and the Protection of Photosynthesis during Water Deficit

2009

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We investigated the effects of P SARK ::IPT (for Senescence-Associated Receptor Kinase::Isopentenyltransferase) expression and cytokinin production on several aspects of photosynthesis in transgenic tobacco (Nicotiana tabacum cv SR1) plants grown under optimal or restricted (30% of optimal) watering regimes. There were no significant differences in stomatal conductance between leaves from wild-type and transgenic P SARK -IPT plants grown under optimal or restricted watering. On the other hand, there was a significant reduction in the maximum rate of electron transport as well as the use of triose-phosphates only in wild-type plants during growth under restricted watering, indicating a biochemical control of photosynthesis during growth under water deficit. During water deficit conditions, the transgenic plants displayed an increase in catalase inside peroxisomes, maintained a physical association among chloroplasts, peroxisomes, and mitochondria, and increased the CO 2 compensation point, indicating the cytokinin-mediated occurrence of photorespiration in the transgenic plants. The contribution of photorespiration to the tolerance of transgenic plants to water deficit was also supported by the increase in transcripts coding for enzymes involved in the conversion of glycolate to ribulose-1,5-bisphosphate. Moreover, the increase in transcripts indicated a cytokinin-induced elevation in photorespiration, suggesting the contribution of photorespiration in the protection of photosynthetic processes and its beneficial role during water stress.

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Role of cytokinins in the regulation of stomatal conductance of wheat seedlings under conditions of rapidly changing local temperature

Cited by 25 publications

References 16 publications

Unravelling the Metabolic Reconfiguration of the Post-Challenge Primed State in Sorghum bicolor Responding to Colletotrichum sublineolum Infection

Unravelling the Metabolic Reconfiguration of the Post-Challenge Primed State in Sorghum bicolor Responding to Colletotrichum sublineolum Infection

Involvement of root ABA and hydraulic conductivity in the control of water relations in wheat plants exposed to increased evaporative demand

Cytokinin-Dependent Photorespiration and the Protection of Photosynthesis during Water Deficit

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