Role of developmental transcription factors in white, brown and beige adipose tissues

Hilton, Catriona; Karpe, Fredrik; Pinnick, Katherine E.

doi:10.1016/j.bbalip.2015.02.003

Cited by 52 publications

(45 citation statements)

References 177 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…White adipocyte differentiation is regulated by positive and negative stimuli, including a variety of hormones, growth factors and transcription factors (Kim et al, 2013a, b;Lee et al, 2016;Rosen and Spiegelman, 2000). Although the molecular mechanism underlying brown adipocyte differentiation has not been as extensively studied as that of white adipocyte differentiation (Choi et al, 2013a;Hilton et al, 2015;Rosen and Spiegelman, 2000), the differentiation process of brown and white adipocytes has a similar transcriptional pattern. Peroxisome proliferator-activated receptor-g (PPAR-g) and C/EBP-a, master transcriptional regulators in white adipocyte differentiation, are also essential factors in brown adipocyte differentiation, and their expression level has been shown to increase during differentiation of brown adipocytes.…”

Section: Introductionmentioning

confidence: 95%

Set7/9, a methyltransferase, regulates the thermogenic program during brown adipocyte differentiation through the modulation of p53 acetylation

Son

Kim

Park

et al. 2016

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 95%

Set7/9, a methyltransferase, regulates the thermogenic program during brown adipocyte differentiation through the modulation of p53 acetylation

Son

Kim

Park

et al. 2016

Molecular and Cellular Endocrinology

View full text Add to dashboard Cite

“…Adipose tissue, which has been divided into two main types, white adipose tissue (WAT) and brown adipose tissue (BAT), is a complex and highly active metabolic organ [1]. WAT, best known for its role in the storage of energy, is distributed mainly in visceral and subcutaneous sites.…”

Section: Introductionmentioning

confidence: 99%

Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Three Types of Rat Adipose Tissue

Zhang

Fan

et al. 2016

IJMS

View full text Add to dashboard Cite

Quantitative real-time PCR (qRT-PCR) is the most classical technique in the field of gene expression study. This method requires an appropriate reference gene to normalize mRNA levels. In this study, the expression stability of four frequently-used reference genes in epididymal white adipose tissue (eWAT), inguinal beige adipose tissue (iBeAT) and brown adipose tissue (BAT) from obese and lean rats were evaluated by geNorm, NormFinder and BestKeeper. Based on the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, the two most stable reference genes were recommended in each type of adipose tissue. Two target genes were applied to test the stability of the reference genes. The geNorm and NormFinder results revealed that GAPDH and 36B4 exhibited the highest expression stabilities in eWAT, while 36B4 and β-actin had the highest expression stabilities in iBeAT and BAT. According to the results of the BestKeeper analysis, 36B4 was the most stable gene in eWAT, iBeAT and BAT, in terms of the coefficient of variance. In terms of the coefficient of correlation, GAPDH, 36B4 and β-actin were the most stable genes in eWAT, iBeAT and BAT, respectively. Additionally, expected results and statistical significance were obtained using a combination of two suitable reference genes for data normalization. In conclusion, 36B4 and GAPDH, in combination, are the best reference genes for eWAT, while 36B4 and β-actin are two most suitable reference genes for both iBeAT and BAT. We recommend using these reference genes accordingly.

show abstract

“…39,40,42 The development and use of positron-emission tomography (PET) allowed for visualization of BAT in adult humans, 43 and in combination with other functional analyses, 44,45 sparked the recent surge in interest in BAT's function in health and disease. Details of WAT and BAT development and gene signature are discussed elsewhere, 36,46,47 but it is important to note that, at least in mice, anatomically defined BAT adipocytes are derived from a cell lineage different from WAT, which instead share a lineage with myocytes (muscle cells). 48,49 Adult human BAT depots are located near the aorta and within the supraclavicular region of the neck.…”

Section: 31mentioning

confidence: 99%

The role of adipose tissue in cancer-associated cachexia

Vaitkus

Celi

2016

Exp Biol Med (Maywood)

View full text Add to dashboard Cite

Adipose tissue (fat) is a heterogeneous organ, both in function and histology, distributed throughout the body. White adipose tissue, responsible for energy storage and more recently found to have endocrine and inflammation-modulatory activities, was historically thought to be the only type of fat present in adult humans. The recent demonstration of functional brown adipose tissue in adults, which is highly metabolic, shifted this paradigm. Additionally, recent studies demonstrate the ability of white adipose tissue to be induced toward the brown adipose phenotype – “beige” or “brite” adipose tissue – in a process referred to as “browning.” While these adipose tissue depots are under investigation in the context of obesity, new evidence suggests a maladaptive role in other metabolic disturbances including cancer-associated cachexia, which is the topic of this review. This syndrome is multifactorial in nature and is an independent factor associated with poor prognosis. Here, we review the contributions of all three adipose depots – white, brown, and beige – to the development and progression of cancer-associated cachexia. Specifically, we focus on the local and systemic processes involving these adipose tissues that lead to increased energy expenditure and sustained negative energy balance. We highlight key findings from both animal and human studies and discuss areas within the field that need further exploration. Impact statement Cancer-associated cachexia (CAC) is a complex, multifactorial syndrome that negatively impacts patient quality of live and prognosis. This work reviews a component of CAC that lacks prior discussion: adipose tissue contributions. Uniquely, it discusses all three types of adipose tissue, white, beige, and brown, their interactions, and their contributions to the development and progression of CAC. Summarizing key bench and clinical studies, it provides information that will be useful to both basic and clinical researchers in designing experiments, studies, and clinical trials.

show abstract

Role of developmental transcription factors in white, brown and beige adipose tissues

Cited by 52 publications

References 177 publications

Set7/9, a methyltransferase, regulates the thermogenic program during brown adipocyte differentiation through the modulation of p53 acetylation

Set7/9, a methyltransferase, regulates the thermogenic program during brown adipocyte differentiation through the modulation of p53 acetylation

Selection of Suitable Reference Genes for Quantitative Real-Time PCR Normalization in Three Types of Rat Adipose Tissue

The role of adipose tissue in cancer-associated cachexia

Contact Info

Product

Resources

About