Role of Dietary Vitamin E in Cadmium-Induced Oxidative Damage in Rabbit’s Blood, Liver and Kidneys

Abstract

The aim of this work was to determine the effect of dietary vitamin E intake on lipid peroxidation (LPO) by measuring thiobarbituric acid reactive substances (TBARS), vitamin E and reduced glutathione (GSH) levels, and glutathione peroxidase (GSH-Px: EC 1.11.1.9) activity in plasma, red blood cells (RBC), livers, and kidneys of rabbits dosed with cadmium (Cd). Six-month-old clinically healthy New Zealand White rabbits (8 in each group) were given tap water only, containing 1 g CdCl2/L, or tap water with CdCl2 plus vitamin E (100 mg dl-alpha-tocopheryl acetate in 0.2 mL corn oil) daily for 30 days. The vitamin E level in the plasma, liver, and kidneys was significantly higher in the control than in the Cd-only group, and TBARS levels were significantly lower. There were no statistical differences between the control and Cd-only groups GSH-Px activities and GSH levels in RBC, liver, and kidneys. Vitamin E levels in plasma, liver, and kidneys and GSH-Px activity in RBC were higher in the vitamin E group than in both control and Cd-only groups. However, the TBARS levels of RBC, liver, and kidneys in vitamin E administered group were decreased. Therefore, the present study demonstrates the effectiveness of vitamin E in reducing oxidative stress in Cd-treated rabbits and suggests that reductions in increased TBARS due to Cd toxicity may be an important factor in the action of vitamin E.

Protective Role of Vitamin C on Sperm Morphology and Biochemical Enzymes in Lead Nitrate Treated Albino Rats

“…In another study, "Turguta et al (29) " reported that administration of the antioxidant agent Vitamin E together with metal resulted in the recovery of malondialdehyde. "Beytut et al (30) " and "Ognjanovic et al (31) " demonstrated reduction in increased lipid peroxidation due to heavy metal toxicity by Vitamin E."Moneim et al (28) " found that synchronous treatment of flaxseed oil with lead acetate caused increase in the level of malondialdehyde, which is in contrast with above findings. We also assessed antioxidant enzyme, glutathione reductase (GR) at the end of two months in testicular tissue of animals of different groups and found that the level of this enzyme was decreased in lead nitrate treated group in comparison to control group because of persistent oxidative stress.…”

Vitamin E protects against oxidative damage caused by cadmium in the blood of rats

“…In contrast, Sarkar et al (6) indicated that the RBC SOD and CAT activities decreased significantly with Cd and the pretreatment with vit E and/or selenium prior to Cd administration partially reversed these changes. In addition, Beytut et al (40) observed that the Cd treatment decreased the GSH-Px activity of RBC. Armutcu et al (41) demonstrated that plasma iron levels were significantly increased in the acetonetreated rats.…”

The Therapeutic Role of Vitamin E or Zinc and Their Mixture Supplementation on Some Heavy Metals-Induced Testicular Functions Injuries in Male Rats.

“…Furthermore, they concluded that vitamin E pretreatment incompletely lessens Pb-induced oxidative stress by changing antioxidant enzymes. [66] and [67] established the efficiency of vitamin E in decreasing oxidative stress in Cd -treated animals and proposed that declines in increased lipid peroxidation due to Cd poisonousness may be an significant factor in the action of vitamin E. [68] presented that the exposure to Cd produced marked elevation in the level of lipid peroxidation and a decline in SOD, glutathione peroxidase (GSH-Px) and CAT activities complemented by an upsurge in the rate of hemoglobin autoxidation in Swiss albino rats. Moreover, they verified that the cure with vitamin E significantly reduced the alterations caused by Cd exposure in all examined parameters.…”

“…It is well-known to have been confirmed beneficial in certain disease courses. Because of the health problems induced by many environmental pollutants, much effort has been expended in evaluating the relative antioxidant potency of vitamin E [11][12][13][14]. Till the first decade of the twenty-one century, there is no information regarding the effect of vitamin E on the testicular toxicities induced by a mixture of heavy metals (Pb, Hg, Cd and Cu) exposure in mice or other mammals [15].…”

“…Antioxidants can also help repair the damage already sustained by cells. [9] Vitamin E and zinc are two of many antioxidants which can block some of the damage caused by free radicals, and can also help reduced the damage to the body which caused by toxic chemicals and pollutants such as heavy metals [10,11].…”