Role of DNA methylation on human CTSG in dermatomyositic myoideum

Peng, Yue; Sun, Xiao‐Feng; Liang, Ying

doi:10.1002/cbin.11447

Cited by 8 publications

(7 citation statements)

References 19 publications

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“…We used both the LASSO-logistic model and SVM-RFE to screen potential biomarkers, which yielded in five genes: CTSG, F13A1, PABPC1, ECHDC2, and BIRC2. CTSG takes part in the development of dermatomyositic myoideum, which is possibly mediated by inflammatory cytokines [45].…”

Section: Discussionmentioning

confidence: 99%

Five Genes as Diagnostic Markers of Ischemic Stroke and Their Correlation with Immune Infiltration

Wang¹,

Zhang²,

Guan³

et al. 2021

Preprint

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Background: This study aimed to identify potential diagnostic markers of ischemic stroke (IS) and discuss the function of immune cell infiltration during the pathological process. Methods: We used IS datasets from the Gene Expression Omnibus database. Differentially expressed genes (DEGs) were identified, and functional correlation analysis was performed. We then screened and verified the diagnostic markers of IS. We evaluated the infiltration of immune cells in infarcts using CIBERSORT and analyzed the correlation between diagnostic markers and infiltrating immune cells. Results: A total of 366 DEGs were screened in this study. Genes encoding CTSG, F13A1, PABPC1, ECHDC2, BIRC2 and infiltrating monocytes, M0 macrophages, activated dendritic cells, and neutrophils (area under the curve [AUC] = 0.945) were identified as diagnostic markers of IS. Immune cell infiltration analysis suggested that memory B cells, regulatory T cells, M0 macrophages, CD8 + T cells, γδT cells, activated natural killer cells, monocytes, activated mast cells, and neutrophils were involved in the IS process. Analysis of correlations between expressed genes and infiltrating immune cells found that CTSG was positively associated with M0 macrophages, F13A1 was positively associated with monocytes, PABPC1 was positively associated with activated dendritic cells, eosinophils were negatively associated with neutrophils, ECHDC2 was negatively associated with monocytes, and BIRC2 was positively associated with eosinophils. Conclusion: five genes and four types of immune cells were identified as diagnostic markers of IS, and immune cell infiltration may play an important role in the progression of IS.

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Section: Discussionmentioning

confidence: 99%

Five Genes as Diagnostic Markers of Ischemic Stroke and Their Correlation with Immune Infiltration

Wang¹,

Zhang²,

Guan³

et al. 2021

Preprint

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“…The conditions of 100 ng/mL tumor necrosis factor-α (TNF-α) (APExBIO, Houston, TX, USA) for 16 h [ 10 ], 20 ng/mL 5-Azacytidine (APExBIO) for 24 h [ 11 ], 1 μM p38/MAPK inhibitor (2-(4-chlorophenyl)-4-(4-fluorophenyl)-5-(pyridin-4-yl)-1H-pyrazol-3(2 H)-one) (APExBIO) for 4 h, 50 mM transforming growth factor β1 (TGFB1) inhibitor (4-[2-(6-methylpyridin-2-yl)-5,6-dihydro-4 H-yrrolo[1,2-b]pyrazol-3-yl]quinoline-6-carboxamide) (APExBIO) for 4 h, 0.1 μM Rho associated coiled-coil containing protein kinase 1/2 (ROCK1/2) (4-[(1 R)-1-aminoethyl]-N-pyridin-4-ylcyclohexane-1-carboxamide) (APExBIO) for 6 h, 10 μM peroxisome proliferator activated receptor gamma/delta (PPARγ/δ) (2,5-dichloro-N-(2-methyl-4-nitrophenyl) benzenesulfonamide) (APExBIO) for 4 h, and 100 μM signal transducer and activator of transcription 3 (STAT3) (5,15-diphenyl-21 H,23 H-porphine) (APExBIO) for 6 h were adopted. Nucleotides for siRNAs of DNMT1, DNMT3A, DNMT3B as previously described [ 12 ]. PRKCA-AS1 RNA interference were synthesized from GenePharma (Shanghai, China).…”

Section: Methodsmentioning

confidence: 99%

“…The conditions of 100 ng/mL tumor necrosis factor-α (TNF-α) (APExBIO, Houston, TX, USA) for 16 h [10], 20 ng/mL 5-Azacytidine (APExBIO) for 24 h [11] (APExBIO) for 6 h were adopted. Nucleotides for siRNAs of DNMT1, DNMT3A, DNMT3B as previously described [12]. PRKCA-AS1 RNA interference were synthesized from GenePharma (Shanghai, China).…”

Section: Cell Culturementioning

confidence: 99%

Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease

Xie

Wang

2021

Bioengineered

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In the present study, mitral valve tissues from three mitral stenosis patients with RHD by valve replacement and two healthy donors were harvested and conducted DNA methylation signature on PRKCA by MeDIP-qPCR. The presence of hypomethylated CpG islands at promoter and 5ʹ terminal of PRKCA was observed in RHD accompanied with highly expressed PRKCA and downregulated antisense long non-coding RNA (lncRNA) PRKCA-AS1 compared to health control. Furthermore, the enrichments of DNMT1/3A/3B on PRKCA were detected by ChIP-qPCR assay in vivo and in human cardiomyocyte AC16 and RL-14 cells exposed to TNF-α in vitro, and both demonstrated that DNMT1 substantially contributed to DNA methylation. Additionally, PRKCA-AS1 was further determined to bind with promoter of PRKCA via 5ʹ terminal and interact with DNMT1 via 3ʹ terminal. Taken together, our results illuminated a novel regulatory mechanism of DNA methylation on regulating PRKCA transcription through lncRNA PRKCA-AS1, and shed light on the molecular pathogenesis of RHD occurrence.

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“…The ChIP assay was performed as previously described (Peng et al, 2020). Briefly, 5 × 10 6 cells were fixed with 1% formaldehyde, quenched with 0.125 M glycine at room temperature and then lysed in 500 μL of lysis buffer (10 mM Tris-HCl (pH 8.0), 10 mM NaCl, and 0.2% IGEPAL CA-630 (Thermo Fisher Scientific)) on ice for 30 min.…”

Section: Chromatin Immunoprecipitation (Chip) Assaymentioning

confidence: 99%

Dihydropyrimidinase like 3 as a novel target of wild type p53 suppresses MAPK pathway in response to hypoxia

Du¹,

Gong²,

Liang³

et al. 2022

Biocell

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Endometrial cancer remains to be a major type of malignancy in threatening female life. Molecular insights in advancing our understanding of endometrial tumorigenesis are much needed. We here report that a less-studied protein Dihydropyrimidinase like 3 (DPYSL3) is a potent tumor suppressor. DPYSL3 is uniquely regulated by wild type p53 (wtp53), and its expression is at the highest level when cells carry wtp53 and are exposed to hypoxia. We reveal that wtp53 can bind DPYSL3 promoter to enhance DPYSL3 expression and in turn, the elevated DPYSL3 can restrain cancer cell proliferation and invasion in vitro and in vivo. Importantly, we observe that DPYSL3 can interfere with MAP kinase pathway, supported by a substantially reduced level of phosphorylated ERK in cells with high expression of DPYSL3. Furthermore, we identify the specific region of DPYSL3 that is responsible for its interaction with MEK and a subsequently reduced activity of ERK. In combination of molecular docking and mutagenesis analysis, we validated that the therapeutic implication of 17 A.A.s of DPYSL3, which can reduce the activity of the MAPK pathway and inhibit endometrial tumor cell growth in vitro and in vivo. Therefore, our study not only demonstrates in-depth understanding of human tumorigenesis, especially endometrial tumor, but also only provides a therapeutic potential to develop an effective tool to fight against human malignancy.

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Role of DNA methylation on human CTSG in dermatomyositic myoideum

Cited by 8 publications

References 19 publications

Five Genes as Diagnostic Markers of Ischemic Stroke and Their Correlation with Immune Infiltration

Five Genes as Diagnostic Markers of Ischemic Stroke and Their Correlation with Immune Infiltration

Coordination of PRKCA/PRKCA-AS1 interplay facilitates DNA methyltransferase 1 recruitment on DNA methylation to affect protein kinase C alpha transcription in mitral valve of rheumatic heart disease

Dihydropyrimidinase like 3 as a novel target of wild type p53 suppresses MAPK pathway in response to hypoxia

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