Role of electrophilic nitrated fatty acids during development and response to abiotic stress processes in plants

Begara-Morales, Juan C.; Mata-Pérez, Capilla; Padilla, María N.; Chaki, Mounira; Valderrama, Raquel; Aranda-Caño, Lorena; Barroso, Juan B.

doi:10.1093/jxb/eraa517

Cited by 12 publications

(5 citation statements)

References 70 publications

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“…Several nitro-derivatives of other fatty acids have also been detected in animals, including nitro-arachidonic acid (NO 2 -AA), nitro-eicosapentaenoic acid (NO 2 -EPA), and nitro-docosahexaenoic acid (NO 2 -DHA) (Milic et al 2015). In plants, NO 2 -FAs have been detected in Brassica napus, Pisum sativum, Oryza sativa, and fresh olives (Begara-Morales et al 2021). These plants are considered an external source of NO 2 -FAs, and supplementing a diet with these foods may help to increase the endogenous levels of NO 2 -FAs (Begara-Morales et al 2021;Fazzari et al 2014).…”

Section: Formation Of No 2 -Fasmentioning

confidence: 99%

“…In plants, NO 2 -FAs have been detected in Brassica napus, Pisum sativum, Oryza sativa, and fresh olives (Begara-Morales et al 2021). These plants are considered an external source of NO 2 -FAs, and supplementing a diet with these foods may help to increase the endogenous levels of NO 2 -FAs (Begara-Morales et al 2021;Fazzari et al 2014). Upon eating nitrate-rich foods (green leafy vegetables, such as spinach and lettuce, and cauliflower and beetroot), the nitrates are reduced to nitrite in the oral cavity (Qin & Wang 2022), protonated in the gastric compartment, and combined with PUFAs from nuts, meat, and dairy products to generate NO 2 -FAs in humans and animals (Rocha et al 2012).…”

Section: Formation Of No 2 -Fasmentioning

confidence: 99%

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Nitro-fatty acids: mechanisms of action, roles in metabolic diseases, and therapeutics

Ni,

Tan,

et al. 2024

Curr Med

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Nitro-fatty acids (NO2-FAs) are a class of bioactive lipids that mediate metabolic, anti-oxidative stress, anti-inflammatory, and other signaling actions. Endogenously, NO2-FAs are derived from the non-enzymatic reactions of unsaturated fatty acids with reactive nitrogen species. The electrophilic properties of the nitro group results in NO2-FAs being able to undergo rapid and reversible reactions with biological nucleophiles, such as cysteine and histidine, thus supporting post-translational modifications of proteins. The reactions of NO2-FAs with biological nucleophiles regulate a range of key signaling pathways involved in gene expression responses, enzyme activity, and cellular processes. In disease animal models, NO2-FAs are produced under conditions of inflammation and oxidative stress and play a protective role in a variety of metabolic diseases, which have been associated with anti-atherosclerosis, blood-pressure lowering, and are involved in the regulation of glycolipid metabolism and insulin resistance. Based on these, more clinical studies might find a correlation between NO2-FAs levels and pathophysiology in patients with metabolic diseases. Importantly, NO2-FAs therapeutics are effective in clinical trials. In addition, dietary supplementation with nitrates and unsaturated fatty acids can endogenously increase NO2-FAs levels in mice and humans. These findings support dietary approaches that increase the endogenous levels of NO2-FAs might potentially reduce the risk of metabolic diseases. To identify the specific mechanism of action and therapeutic potential of NO2-FAs, we have summarized the main mechanisms of action of NO2-FAs in metabolic disease progression to provide insights for the development of new therapeutics for metabolic diseases.

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Section: Formation Of No 2 -Fasmentioning

confidence: 99%

Section: Formation Of No 2 -Fasmentioning

confidence: 99%

Nitro-fatty acids: mechanisms of action, roles in metabolic diseases, and therapeutics

Ni,

Tan,

et al. 2024

Curr Med

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“…In plants, nitro-oleic acid and nitro-linolenic acid have been mainly characterized, with the latter having the ability to modulate the expression of salinity abiotic stress-related, Cd 2+ toxicity and wounding genes in Arabidopsis . In addition, other properties described for these nitro-fatty acids include their role as NO· donors, with the possibility of inducing RNS-based PTMs on specific proteins ( Begara-Morales et al., 2019 ; Mata-Pérez et al., 2020 ; Begara-Morales et al., 2021 ), which will be described in more detail later.…”

Section: Reactive Species: Biogenesis Interplay and Antioxidant Defensementioning

confidence: 99%

Redox post-translational modifications and their interplay in plant abiotic stress tolerance

et al. 2022

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The impact of climate change entails a progressive and inexorable modification of the Earth’s climate and events such as salinity, drought, extreme temperatures, high luminous intensity and ultraviolet radiation tend to be more numerous and prolonged in time. Plants face their exposure to these abiotic stresses or their combination through multiple physiological, metabolic and molecular mechanisms, to achieve the long-awaited acclimatization to these extreme conditions, and to thereby increase their survival rate. In recent decades, the increase in the intensity and duration of these climatological events have intensified research into the mechanisms behind plant tolerance to them, with great advances in this field. Among these mechanisms, the overproduction of molecular reactive species stands out, mainly reactive oxygen, nitrogen and sulfur species. These molecules have a dual activity, as they participate in signaling processes under physiological conditions, but, under stress conditions, their production increases, interacting with each other and modifying and-or damaging the main cellular components: lipids, carbohydrates, nucleic acids and proteins. The latter have amino acids in their sequence that are susceptible to post-translational modifications, both reversible and irreversible, through the different reactive species generated by abiotic stresses (redox-based PTMs). Some research suggests that this process does not occur randomly, but that the modification of critical residues in enzymes modulates their biological activity, being able to enhance or inhibit complete metabolic pathways in the process of acclimatization and tolerance to the exposure to the different abiotic stresses. Given the importance of these PTMs-based regulation mechanisms in the acclimatization processes of plants, the present review gathers the knowledge generated in recent years on this subject, delving into the PTMs of the redox-regulated enzymes of plant metabolism, and those that participate in the main stress-related pathways, such as oxidative metabolism, primary metabolism, cell signaling events, and photosynthetic metabolism. The aim is to unify the existing information thus far obtained to shed light on possible fields of future research in the search for the resilience of plants to climate change.

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“…This is not the case of the second mechanism, which involves the formation of carbon-centered radicals by the direct addition of a NO 2 radical. This radical can react with another NO 2 radical to form an unstable nitro-nitrite or dinitro compound, which will rapidly decompose to release nitrous acid (HNO 2 ) and generate a NO 2 -FA [ 1 , 3 ].…”

Section: Introductionmentioning

confidence: 99%

Nitrated Fatty-Acids Distribution in Storage Biomolecules during Arabidopsis thaliana Development

et al. 2022

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The non-enzymatic interaction of polyunsaturated fatty acids with nitric oxide (NO) and derived species results in the formation of nitrated fatty acids (NO2-FAs). These signaling molecules can release NO, reversibly esterify with complex lipids, and modulate protein function through the post-translational modification called nitroalkylation. To date, NO2-FAs act as signaling molecules during plant development in plant systems and are involved in defense responses against abiotic stress conditions. In this work, the previously unknown storage biomolecules of NO2-FAs in Arabidopsis thaliana were identified. In addition, the distribution of NO2-FAs in storage biomolecules during plant development was determined, with phytosterol esters (SE) and TAGs being reservoir biomolecules in seeds, which were replaced by phospholipids and proteins in the vegetative, generative, and senescence stages. The detected esterified NO2-FAs were nitro-linolenic acid (NO2-Ln), nitro-oleic acid (NO2-OA), and nitro-linoleic acid (NO2-LA). The last two were detected for the first time in Arabidopsis. The levels of the three NO2-FAs that were esterified in both lipid and protein storage biomolecules showed a decreasing pattern throughout Arabidopsis development. Esterification of NO2-FAs in phospholipids and proteins highlights their involvement in both biomembrane dynamics and signaling processes, respectively, during Arabidopsis plant development.

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Role of electrophilic nitrated fatty acids during development and response to abiotic stress processes in plants

Cited by 12 publications

References 70 publications

Nitro-fatty acids: mechanisms of action, roles in metabolic diseases, and therapeutics

Nitro-fatty acids: mechanisms of action, roles in metabolic diseases, and therapeutics

Redox post-translational modifications and their interplay in plant abiotic stress tolerance

Nitrated Fatty-Acids Distribution in Storage Biomolecules during Arabidopsis thaliana Development

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