Role of elements and physiologically active compounds in the regulation of synthesis and accumulation of indole alkaloids in Catharanthus roseus L.

Lovkova, M. Ya.; Бузук, Г. Н.; Sokolova, S. M.; Buzuk, L. N.

doi:10.1007/s10438-005-0050-4

Cited by 11 publications

(3 citation statements)

References 16 publications

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“…Addition of tetramethylammonium bromide induced positive response by increasing ajmalicine content in shake flask and bioreactor [ 9 ]. Effects of a number of elements as Co, Ni, Cr, Cu, B, Mo, Fe, and Vn produced variable response on alkaloid content of 4-6 day old seedlings of Catharanthus [ 10 ]. It is not clear whether metal elicitation studied at cellular level may actually replicate at whole plant level.…”

mentioning

confidence: 99%

Influence of some heavy metals on growth, alkaloid content and composition in Catharanthus roseus L.

Srivastava¹,

Srivastava²

2010

Indian J Pharm Sci

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Shoot biomass production, alkaloid content and composition as influence by cadmium, manganese, nickel and lead at uniform dose of 5 mM were investigated in Catharanthus roseus plants grown in sand culture. Treatment with Mn, Ni, and Pb significantly enhanced total root alkaloid accumulation. Cd and Ni treatment resulted in two-fold where as Pb treatment resulted in three fold increase in serpentine content of roots. The non-significant affect on biomass suggests that plants can withstand metal stress at the level tested with positive affect on root alkaloid content.

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mentioning

confidence: 99%

Influence of some heavy metals on growth, alkaloid content and composition in Catharanthus roseus L.

Srivastava¹,

Srivastava²

2010

Indian J Pharm Sci

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“…This research group also analysed the changes in the reactive oxygen metabolism of C. roseus in terms of H 2 O 2 content, lipid peroxidation and the free‐radical quenching systems (non‐enzymatic and enzymatic antioxidants) under drought stress and found that ajmalicine was significantly increased over the control [73–75]. Lovkova et al [76] studied the effect of various elements (Co, Ni, Zn, W, Mn, Cr, Cu, B, Mo, Fe and V) and physiologically active compounds, including natural and synthetic auxins, cytokinins and gibberellin, at increasing concentrations, on the synthesis and accumulation of IAs in C. roseus . It was demonstrated that auxin down‐regulated the transcription level of the Str gene in C. roseus cell cultures [77].…”

Section: Effects Of Elicitor On Tia Accumulation In C Roseusmentioning

confidence: 99%

Production and metabolic engineering of terpenoid indole alkaloids in cell cultures of the medicinal plant Catharanthus roseus (L.) G. Don (Madagascar periwinkle)

Zhou

Shao

Tang

2009

Biotech and App Biochem

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The Madagascar periwinkle [Catharanthus roseus (L.) G. Don] is a plant species known for its production of TIAs (terpenoid indole alkaloids), many of which are pharmaceutically important. Ajmalicine and serpentine are prescribed for the treatment of hypertension, whereas the bisindoles vinblastine, vincristine and 3',4'-anhydrovinblastine are used for their antineoplastic activity in the treatment of many cancers. However, TIAs are produced in small yields in C. roseus, which make them expensive. Cell and metabolic engineering has focused on increasing flux through the TIA pathway by various means, including optimization of medium composition, elicitation, construction of noval culture systems and introduction of genes encoding specific metabolic enzymes into the C. roseus genome. The present review will attempt to present the state-of-the-art of research in this area and provide an update on the cell and metabolic engineering of TIAs in C. roseus. We hope that this will contribute to a better understanding of the ways in which TIA production can be achieved in different C. roseus culture systems.

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“…often referred to as elicitators, have widely been applied to improve secondary metabolite synthesis in cultivated tissues (Dicosmo and Miswa 1985;Moreno-Valenzuela et al 2003;Zheng and Wu 2004;Junaid et al 2011). Application of various chemicals like osmotic shock, salt stress, elements has been utilized to improve alkaloid yield in cell cultures of C. roseus (Zhao et al 2000(Zhao et al , 2001Lovkova et al 2005;Zahid and Mujib 2012). Some of the enrichment responses of those treatments, although were cell-line specific and often limit their utilization, studies using efficient biotic/abiotic elicitors for improving secondary metabolite synthesis are an important approach in cell cultures (Moreno et al 1995).…”

Section: Introductionmentioning

confidence: 99%

NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities

Fatima

Mujib

Tonk

2015

Plant Cell Tiss Organ Cult

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An improved synthesis of vinblastine and vincristine was observed in Catharanthus roseus L. (G). Don by using NaCl as an elicitor. Various in vitro grown embryogenic tissues were cultivated under salinity stress for enhanced synthesis of alkaloids. Different levels of salt [control (0 mM), NT1 (25 mM), NT2 (50 mM), NT3 (75 mM), NT4 (100 mM), and NT5 (125 mM)] were amended in MS and callus biomass growth (fresh-and dryweight) and biochemical attributes at various embryogenic stages were studied. Maximum callus biomass reduction was observed in 125 mM NaCl amended medium. Antioxidant enzymes i.e. superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase and glutathione reductase activities were assayed as in vitro grown tissues were elicitated with NaCl, causing cellular stress. The antioxidant enzymes activity increased linearly with increasing NaCl level in medium, 4.97 EU min -1 mg -1 SOD and 3.14 EU min -1 mg -1 CAT, both being maximum in proliferating embryos at NT5. Quantitative estimation and comparative yield of alkaloids were made in response to NaCl stress in different cultivated tissues by using HPTLC method. Vinblastine content was observed to be maximum in regenerated leaves (14.17 mg/g dry wt) on 25 mM NaCl amended medium, followed by in vitro raised shoots. Similarly, better accumulation of vincristine (5.12 mg/g dry wt) was also noted in NaCl amended medium especially at low level (NT1). The data presented indicate that the synthesis of Catharanthus alkaloids was growth specific and was influenced by NaCl levels.

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Role of elements and physiologically active compounds in the regulation of synthesis and accumulation of indole alkaloids in Catharanthus roseus L.

Cited by 11 publications

References 16 publications

Influence of some heavy metals on growth, alkaloid content and composition in Catharanthus roseus L.

Influence of some heavy metals on growth, alkaloid content and composition in Catharanthus roseus L.

Production and metabolic engineering of terpenoid indole alkaloids in cell cultures of the medicinal plant Catharanthus roseus (L.) G. Don (Madagascar periwinkle)

NaCl amendment improves vinblastine and vincristine synthesis in Catharanthus roseus: a case of stress signalling as evidenced by antioxidant enzymes activities

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