Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: A redox proteomic investigation

Bouallegui, Younes; Younes, Ridha Ben; Oueslati, Ridha; Sheehan, David

doi:10.1016/j.aquatox.2018.04.013

Cited by 26 publications

(18 citation statements)

References 58 publications

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“…Given that citrate-coated nAg would release ionic Ag and produce oxidative stress, the different effects observed with bPEI-and Si-coated nAg could be related to mechanisms other than ionic Ag. The effects of citratecoated nAg on protein carbonylation (oxidation) and ROS activity were observed in mussels and involved the release of ionic Ag, in part at least, and oxidative stress [7,8,24]. However, endocytosis pathways and autophagy could also modulate the effects of nAg in cells [25].…”

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confidence: 99%

The Influence of Surface Coatings of Silver Nanoparticles on the Bioavailability and Toxicity to Elliptio complanata Mussels

Auclair

Turcotte

Gagnon

et al. 2019

Journal of Nanomaterials

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Nanomaterials could be modified with various coatings which could modulate their behavior in the environment, bioavailability and toxicity. The purpose of this study was to determine if the selected coatings of silver nanoparticles (nAg) could influence the fate, bioavailability, and toxicity toward suspension feeding freshwater mussels, Elliptio complanata. Mussels were exposed for 96 h to 50 μg/L of nAg with the following surface coatings: citrate, silicate (Si), polyvinylpyrrolidone (PVP), and branched polyethylenimine (bPEI). After the exposure period, mussels were analyzed for total Ag, resistance to air emersion, oxidative stress, genotoxicity, and autophagosome protein uptake (protein ubiquitinylation) in gills and digestive glands. The data revealed that citrate- and PVP-coated nAg were 2 times more abundant in the digestive gland compared to bPEI- and Si-coated nAg with estimated bioaccumulation factors between 5 and 10. The data revealed that tissue Ag levels were closely associated with air survival time, weight loss during air exposure, DNA strand breaks, LPO, and protein-ubiquitin levels in the digestive gland. The data supports the hypothesis that the coatings could influence bioavailability and toxicity in freshwater mussels.

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confidence: 99%

The Influence of Surface Coatings of Silver Nanoparticles on the Bioavailability and Toxicity to Elliptio complanata Mussels

Auclair

Turcotte

Gagnon

et al. 2019

Journal of Nanomaterials

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“…This would be a mechanism requiring silver uptake / transport in the mussel foot where adhesive proteins are synthetized, that although we did not verify, it is likely to occur. To this end, reduced thiols (free-thiol oxidation) were reported in mussels tissues exposed to < 50 nm AgNP for just 12h (Bouallegui et al, 2018). We can argue, nonetheless, a second mechanism for byssal adhesion impairment, as functional silky mussel threads establish histidine-mediated intermolecular binding of metals such as zinc at the level of the collagenous stalk (Qin and White, 1998) as well as metal-catecholate complexes at the adhesive plaque matrix protein mfp-1 (Xu, 2013).…”

Section: Discussionmentioning

confidence: 99%

Ecotoxicological effects of silver nanoparticles in marine mussels

Calisi

Lorusso

Gallego-Urrea

et al. 2021

Preprint

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In the marine bioindicator species M. galloprovincialis Lam we predicted toxicity and bioaccumulation of 5 nm alkane-coated and 50 nm uncoated silver nanoparticles (AgNPs) along with Ag+, as a function of the actual dose level. We generated a time persistence model of silver concentration in seawater and used the Area Under the Curve (AUC) as independent variable in hazard assessment. This approach allowed us to evaluate unbiased ecotoxicological endpoints for acute (survival) and chronic toxicity (byssal adhesion). Logistic regression analysis rendered LC5096h values of 0.68 ± 0.08; 1.00 ± 0.20; 1.00 ± 0.42 mg h L-1 respectively for Ag+, 5 nm and 50 nm AgNP posing no evidence the silver form is a necessary variable to predict the survival outcome. By contrast, for byssal adhesion regression analysis revealed a much higher toxicological potential of Ag+ vs AgNPs, 0.0021 ± 0.0009; 0.053 ± 0.016; 0.021 (no computable error for 50 nm AgNP) mg h L-1, and undoubtedly confirmed a role of the silver form. Bioaccumulation was higher for Ag+ > 5 nm AgNP > 50 nm AgNP reflecting a parallel with the preferential uptake route / target organ. We, eventually, provided a full range of toxicological endpoints to derive risk quotients.

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“…The metallic nature of NPs can stimulate an oxidative stress in different organs of animals. The degree of ROS toxicity in various tissues can be function-specific as well as NP composition-specific depending upon the nature of metallic ions release [64,65,66]. Oxidative stress has also been reported by Wise et al [61] as the causative agent for disintegrating cell membranes and biomolecules.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Assessment of Nanotoxicity Induced by Metal (Silver, Nickel) and Metal Oxide (Cobalt, Chromium) Nanoparticles in Labeo rohita

et al. 2019

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In the present in vivo study, we provide a comparison of toxicological consequences induced by four different types of spherical nanoparticles (NPs)—silver nanoparticles (AgNPs, 40 ± 6 nm), nickel (NiNPs, 43 ± 6 nm), cobalt oxide (Co3O4NPs, 60 ± 6 nm), and chromium oxide (Cr3O4NPs, 50 ± 5 nm)—on freshwater fish Labeo rohita. Fish were exposed to NPs (25 mg/L) for 21 days. We observed a NPs type-dependent toxicity in fish. An altered behavior showing signs of stress and a substantial reduction in total leukocyte count was noticed in all NP-treated groups. A low total erythrocyte count in all NP-treated fish except for Co3O4NPs was discerned while a low survival rate in the case of Cr3O4NP-treated fish was observed. A significant decrease in growth and hemoglobin were noticed in NiNP- and Cr3O4NP-treated fish. A considerable total protein elevation was detected in NiNP-, Co3O4NP-, and Cr3O4NP-treated groups. An upgrading in albumin level was witnessed in Co3O4NP- and Cr3O4NP-treated groups while a high level of globulin was noted in NiNP- and Co3O4NP-exposed groups. In all NP-treated groups, a depleted activity of antioxidative enzymes and pathological lesions in liver and kidney were noticed.

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Role of endocytotic uptake routes in impacting the ROS-related toxicity of silver nanoparticles to Mytilus galloprovincialis: A redox proteomic investigation

Cited by 26 publications

References 58 publications

The Influence of Surface Coatings of Silver Nanoparticles on the Bioavailability and Toxicity to Elliptio complanata Mussels

The Influence of Surface Coatings of Silver Nanoparticles on the Bioavailability and Toxicity to Elliptio complanata Mussels

Ecotoxicological effects of silver nanoparticles in marine mussels

A Comparative Assessment of Nanotoxicity Induced by Metal (Silver, Nickel) and Metal Oxide (Cobalt, Chromium) Nanoparticles in Labeo rohita

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