Role of Ethylene in Axillary Shoot Proliferation of Lavandin—Interaction with Benzyladenine and Polyamines

Panizza, M.; Mensuali-Sodi, A.; Tognoni, F.

doi:10.1093/jxb/44.2.387

Cited by 19 publications

(9 citation statements)

References 30 publications

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“…The supply of CO 2 from external atmosphere is facilitated in vessels with a high ventilation rate which also reduces the accumulation of ethylene synthesized by plant tissues and/or released by  Submitted 13 November 2013, last revision 1 February 2014, accepted 20 March 2014. Abbreviations: CAD -caffeic acid derivative; P N -net phothosynthetic rate; PPFD -photosynthetic photon flux density; RA -rosmarinic acid; R D -dark respiration rate abiotic sources, such as agar and plastic materials (Panizza et al 1993, Xiao et al 2011. A high ethylene concentration can influence plantlet morphogenesis and induce chlorophyll breakdown, thus affecting negatively their photosynthesis (Hazarika 2006).…”

Section: Introductionmentioning

confidence: 99%

In vitro culture of sweet basil: gas exchanges, growth, and rosmarinic acid production

Kiferle¹,

Lucchesini²,

Maggini³

et al. 2014

Biologia plant.

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Five in vitro culture systems with different ventilation rates were used to investigate the influence of vessel environment on photosynthesis, dark respiration, ethylene evolution, and rosmarinic acid (RA) production in sweet basil (Ocimum basilicum L.) micropropagated shoots. The systems under comparison were two bioreactors with either temporary (RITA™) or stationary (Growtek™) immersion, and three types of vessels (Magenta™, Microbox ECO2™, and PCCV25™) that are largely used for plant micropropagation. Shoots of green-leaved cv. Genovese and purple-leaved cv. Dark Opal were cultured on a modified Murashige and Skoog medium containing 0.25 mg dm−3 6-benzylaminopurine. The instantaneous rates of photosynthesis, dark respiration, and ethylene production were determined by gas chromatography measuring CO2 and ethylene concentrations in vessel headspaces. The tissue RA content was determined by HPLC in HCl-methanol extracts. The explant growth and morphology were significantly affected by culture conditions and cultivars. The largest biomass production was observed under the photomixotrophic culture conditions provided by Growtek™, whereas the highest RA content in shoot tissues was found in the RITA™ photomixotrophic system, where ethylene accumulated to the greatest extent

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Section: Introductionmentioning

confidence: 99%

In vitro culture of sweet basil: gas exchanges, growth, and rosmarinic acid production

Kiferle¹,

Lucchesini²,

Maggini³

et al. 2014

Biologia plant.

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“…In some species, axillary shoot induction and development appear to be positively influenced by the stimulation of the hormone's biosynthesis (Van Aartrijk et al, 1985;Van Dijck et al, 1988;Panizza et al, 1993), and the supply of products inhibiting its synthesis or action has negative effects (Dimasi-Theriou et al, 1993). In some species, axillary shoot induction and development appear to be positively influenced by the stimulation of the hormone's biosynthesis (Van Aartrijk et al, 1985;Van Dijck et al, 1988;Panizza et al, 1993), and the supply of products inhibiting its synthesis or action has negative effects (Dimasi-Theriou et al, 1993).…”

Section: Discussionmentioning

confidence: 99%

In vitro axillary shoot proliferation of apple rootstocks under different ethylene conditions

Lambardi¹,

Benelli̇²,

Fabbri³

1997

In Vitro Cell.Dev.Biol.-Plant

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Ethylene effect on in vitro shoot proliferation of two apple rootstocks, MM 111 and M9, was studied. Ethylene biosynthesis was proportionally stimulated by increasing concentrations of the precursor 1-aminocyclopropane-l-carboxylic acid (ACC). When 25 D.M or more ACC was applied without any control of the headspace of culture vessels, shoot proliferation of both rootstocks was negatively affected. However, when shoot cultures were transferred to ACC-supplemented medium after the second week of culture, ACC had no effect. Supplementing the medium with aminoethoxyvinylglycine (AVG), an inhibitor of ethylene biosynthesis, together with the application of gas traps inside the flasks, significantly enhanced axillary shoot formation and elongation. Steady and high exogenous concentrations of ethylene in the culture flasks had negative effects on shoot proliferation. MM111 appeared to be more sensitive to ethylene than M9. For AVG a threshold dose was noticed, beyond which phytotoxic effects were induced.

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“…However, as it was demonstrated in the present study, enhanced shoot formation can be achieved also on a hormone-free nutrient medium merely by sealing of culture tubes. In the given context, particular attention should be paid to the gaseous plant hormone ethylene, since Panizza et al [21], working with the in vitro culture of lavandin, have reported on the essential role of ethylene in cytokinin-inducible shoot proliferation. Although the exact levels of ethylene gas inside the culture tubes were not measured during the present study, the fact that additional sealing of culture vessels lead to increased amounts of accumulated ethylene is thoroughly established by other plant tissue culturists [14].…”

Section: Methodsmentioning

confidence: 99%

“…while accumulation of ethylene in culture vessels is also recommended by some of the studies conducted on other plant species [7,21], it is by far not a common consensus among plant biologists. Many authors [17,18,20,22,35] concluded that ethylene accumulation and subsequent action in culture vessels should be restricted by ventilation, absorption, or the inhibition of either ethylene synthesis or action, in order to avoid ethylene-triggered impairment of shoot formation and development.…”

Section: Introductionmentioning

confidence: 99%

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

Žiauka

Kuusienė

2014

Acta Biologica Hungarica

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the present study explored an alternative approach for poplar micropropagation, based on the restriction of gas exchange between inside and outside environments of culture vessel, rather than on the application of exogenous hormones. Apical and nodal stem segments (explants) excised from in vitro-developed shoots of hybrid white poplar (Populus alba L. × P. tremula L.) were incubated in either sealed (with Parafilm) or unsealed capped glass culture tubes (150 × 20 mm) on a hormone-free Woody Plant Medium. Shoot proliferation on apical explants was observed in sealed culture tubes but not in the unsealed ones; the difference between these two samples in respect of shoot number increased in the course of time and became threefold after three months of culture, with 3.2 ± 0.4 (mean ± SE) shoots per explant in the sealed tubes versus 1.1 ± 0.1 in the unsealed ones (for comparison, the mean shoot numbers on nodal explants were 2.4 ± 0.3 and 3.4 ± 0.4 in the unsealed and sealed culture tubes, respectively). Moreover, the shoots taken from the sealed culture tubes could be distinguished by superior shoot length, if compared to the shoots from the unsealed tubes, during the subsequent culture stage under uniform conditions.

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Role of Ethylene in Axillary Shoot Proliferation of Lavandin—Interaction with Benzyladenine and Polyamines

Cited by 19 publications

References 30 publications

In vitro culture of sweet basil: gas exchanges, growth, and rosmarinic acid production

In vitro culture of sweet basil: gas exchanges, growth, and rosmarinic acid production

In vitro axillary shoot proliferation of apple rootstocks under different ethylene conditions

Multiplication and growth of hybrid poplar(Populus alba × P. tremula)shoots on a hormone-free medium

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