Role of Exosomal MicroRNAs and Their Crosstalk with Oxidative Stress in the Pathogenesis of Osteoporosis

Lü, Jun; Zhang, Yan; Liang, Jinqi; Diao, Jiayu; Liu, Peilong; Zhao, Hongmou

doi:10.1155/2021/6301433

Cited by 14 publications

(10 citation statements)

References 98 publications

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“…The disease is related to an imbalance between the number and function of osteoblasts and osteoclasts. Moreover, angiogenesis, inflammation, oxidative stress and miRNAs have been involved in the process of osteoporosis (Li et al, 2018;Lu et al, 2021). It was reported that MSC-Exos could promote osteogenesis of BMSCs and promote the proliferation of osteoblasts to alleviate osteoporosis (Qi et al, 2016;Zhao et al, 2018).…”

Section: Improving Osteoporosismentioning

confidence: 99%

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Liang

Han

Hai

et al. 2022

Front. Cell Dev. Biol.

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Exosomes are extracellular vesicles formed by various donor cells that regulate gene expression and cellular function in recipient cells. Exosomes derived from mesenchymal stem cells (MSC-Exos) perform the regulatory function of stem cells by transporting proteins, nucleic acids, and lipids. Intervertebral disc degeneration (IDD) is one of the main causes of low back pain, and it is characterized by a decreased number of nucleus pulposus cells, extracellular matrix decomposition, aging of the annulus fibrosus, and cartilage endplate calcification. Besides, nutrient transport and structural repair of intervertebral discs depend on bone and cartilage and are closely related to the state of the bone. Trauma, disease and aging can all cause bone injury. However, there is a lack of effective drugs against IDD and bone injury. Recent MSC-Exos fine tuning has led to significant progress in the IDD treatment and bone repair and regeneration. In this review, we looked at the uniqueness of MSC-Exos, and the potential treatment mechanisms of MSC-Exos with respect to IDD, bone defects and injuries.

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Section: Improving Osteoporosismentioning

confidence: 99%

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Liang

Han

Hai

et al. 2022

Front. Cell Dev. Biol.

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“…In recent years, osteoporosis has become a serious healthcare concern for the aging population [ 20 , 21 ]. It is projected that more than about 50% of all osteoporotic hip fractures will occur in Asia by the year 2050.…”

Section: Discussionmentioning

confidence: 99%

Cryptotanshinone Suppressed Postmenopausal Osteoporosis by Preventing RANKL-Mediated Osteoclastogenesis against Kidney Injury

Yang

Han

Gong

et al. 2022

Evidence-Based Complementary and Alternative Medicine

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Background. Cryptotanshinone (CPT), an active component extracted from the root of Salvia miltiorrhiza Bunge, exhibits extensive favorable bioactive properties including anti-inflammatory, antioxidative, antibacterial, and antitumor effects. This study aims to investigate the effects of CPT on osteogenesis and explore related mechanisms both in vivo and in vitro. Methods. In the in vivo experiment, ovariectomized (OVX) female rats were intragastrically administered with CPT at doses of 10 mg/kg and 20 mg/kg for 13 consecutive weeks. Dual-energy X-ray absorptiometry was employed to detect bone mineral density (BMD). ELISA assay was leveraged to detect the biochemical parameters such as BUN and creatinine in the kidney samples. Bone and kidney sections were dyed by H&E and Masson staining kits. In the in vitro experiment, the RAW 264.7 cells were stimulated through the receptor activation of the nuclear factor kappa B ligand (RANKL) to establish an osteoclast differentiation model, and CPT’s protective effect against bone loss was evaluated. Differentiated osteoclasts were determined by TRAP staining. While, osteoclast-marker proteins such as NFATc1, c-Fos, and cathepsin K were identified by Western blot. Results. The results from in vivo experiments revealed that CPT could elevate bone mass and increase bone formation markers in OVX rats. Intriguingly, CPT administration noticeably ameliorated the kidney injury in OVX rats by suppressing BUN and restoring creatinine levels. Furthermore, the results from in vitro experiments suggested that CPT downregulated the protein expression of osteoclast-associated genes such as cathepsin K, c-Fos, and NFATc1 which hinted the related potential mechanisms. Conclusion. The evidence from in vivo and in vitro experiments suggested that CPT exerted antiosteoclastogenic effects by inhibiting the activation of osteoclast differentiation followed by suppressing the protein expressions of cathepsin K, c-Fos, and NFATc1 in osteoclast precursors, and it exhibited protective effects against kidney damage, which highlighted its advantage in clinical application.

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“…Exosomes carry nucleic acids, proteins, lipids, and metabolites that can target receptor cells to be taken up through membrane fusion or endocytosis. In addition, exosomes can cross barriers, such as the blood-brain barrier, which makes them natural vectors in vivo ( Ha et al., 2016 ; Lu et al., 2021 ). Recently, exosomes have been utilized as non-viral vectors for RNA delivery, which indicates their high biocompatibility, low clearance, and suitability for targeting cells ( Duan et al., 2021 ).…”

Section: Ncrna Delivery Scaffolds For Bone Defect Therapymentioning

confidence: 99%

Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions

Guan¹,

Zhang²,

Wu³

2022

iScience

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Role of Exosomal MicroRNAs and Their Crosstalk with Oxidative Stress in the Pathogenesis of Osteoporosis

Cited by 14 publications

References 98 publications

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Mechanism of Action of Mesenchymal Stem Cell-Derived Exosomes in the Intervertebral Disc Degeneration Treatment and Bone Repair and Regeneration

Cryptotanshinone Suppressed Postmenopausal Osteoporosis by Preventing RANKL-Mediated Osteoclastogenesis against Kidney Injury

Non-coding RNA delivery for bone tissue engineering: Progress, challenges, and potential solutions

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