Role of fliC on biofilm formation, adhesion, and cell motility in Cronobacter malonaticus and regulation of luxS

Ling, Na; Wang, Xin; Liu, Dengyu; Shen, Yizhong; Zhang, Danfeng; Ou, Dexin; Fan, Huiying; Wang, Juan; Ding, Yu; Zhang, Jumei; Wu, Qingping; Ye, Yingwang

doi:10.1016/j.fct.2020.111940

Cited by 16 publications

(8 citation statements)

References 54 publications

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“…The 50 lL of two isolates (WT and DluxS ) were inoculated with 4.95 mL of sterile fresh LB at pH 3, 4 and 7 for incubation at 37°C for 16 h with shaking at 180 rpm. Afterwards, cells fixed on the cell climbing slice were analysed using an S-3000 N scanning electron microscope (SEM, Hitachi S4700, Japan) and H7500 Transmission Electron Microscope (TEM, JEL-1230, Japan), which performed as described previously (Ling et al 2021b), respectively.…”

Section: Detection Of Bacterial Morphology Of C Malonaticus Wt and Lu...mentioning

confidence: 99%

Uncovering phenotypic changes of luxS‐mediated acid tolerance in Cronobacter malonaticus

Wang,

Ling,

et al. 2024

Int J of Dairy Tech

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Cronobacter spp. are high acid resistance pathogens classified as Class A pathogens in powdered infant formula. Herein, we investigated the function of Cronobacter luxS in acid response. Results showed that luxS significantly increased the resistance to acid, demonstrating bacterial survival, bacterial morphology and biofilm formation. Furthermore, LuxS might enhance acid response by mediating the up‐regulation of 5‐methyltetrahydropteroyltriglutamate–homocysteine S‐methyltransferase and homocysteine S‐methyltransferase and the down‐regulation of methionine adenosyltransferase in the quorum sensing pathway. Therefore, the quorum sensing pathway mediated by luxS is the key control target for Cronobacter survival in acidic environments.

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Section: Detection Of Bacterial Morphology Of C Malonaticus Wt and Lu...mentioning

confidence: 99%

Uncovering phenotypic changes of luxS‐mediated acid tolerance in Cronobacter malonaticus

Wang,

Ling,

et al. 2024

Int J of Dairy Tech

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“…To determine the roles of LuxS on morphological changes of C. malonaticus in a hypertonic environment, WT and ΔluxS strains were cultured in LB broth with 1.5%, 3.5% and 5.5% NaCl at 37 °C for 16 h. Then, C. malonaticus was imaged by scanning electron microscopy (SEM, S4700; Hitachi, Tokyo, Japan) at 15 kV voltage and observed by a transmission electron microscope (TEM, JEL-1230, Japan Electronics Co. Ltd.) performed as described by Ling et al (2021b) and Wang et al (2013), respectively, with slight modifications.…”

Section: Role Of Luxs In C Malonaticus On the Morphological Changes U...mentioning

confidence: 99%

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Ling

Tong

et al. 2023

Int J of Dairy Tech

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Cronobacter malonaticus is a highly hyperosmotic‐resistant pathogen classified as Class A pathogen by the Food and Agriculture Organization of the United Nations (FAO). This study explored the roles of LuxS as a quorum‐sensing transcription factor in C. malonaticus to tolerate hyperosmotic stress, illustrating that LuxS contributed to survival, biofilm formation and reduced cellular injury when grown with 5.5% NaCl stress. Comparative proteomics between C. malonaticus WT and ΔluxS demonstrated that differentially expressed proteins (DEPs) were abundant in Fe‐S cluster proteins and oxidoreductases, suggesting a potential cross‐tolerance mediated by LuxS could contribute to resisting hyperosmotic stress. Herein, these findings will provide valuable information on LuxS‐mediated osmotic tolerance to controlling C. malonaticus contamination.

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“…Motility interception is a new perspective to prevent and control adhesion. Ling et al (2021) reported that the interception of fliC genes in Cronobacter malonaticus can control motility and adhesion on abiotic surfaces. Other genes such as the luxS genes, responsible for the QS system and flagellin (flgM), are negative regulators of flagellar biosynthesis, which is also important for motility (Ling et al, 2021).…”

Section: Bacterial Adhesion On Dry Surfacesmentioning

confidence: 99%

“…Bacillus subtilis , E. coli , L. monocytogenes , Pseudomonas aeruginosa , and Salmonella are motile and have already been studied, in regards to their motility and adhesion onto wet surfaces. However, further studies with these species and considering dry surfaces need to be conducted (Ling et al., 2021).…”

Section: Dsbs In the Food Industrymentioning

confidence: 99%

Dry surface biofilms in the food processing industry: An overview on surface characteristics, adhesion and biofilm formation, detection of biofilms, and dry sanitization methods

Alonso

Gonçalves

Brito

et al. 2022

Comp Rev Food Sci Food Safe

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Bacterial biofilm formation in low moisture food processing (LMF) plants is related to matters of food safety, production efficiency, economic loss, and reduced consumer trust. Dry surfaces may appear dry to the naked eye, however, it is common to find a coverage of thin liquid films and microdroplets, known as microscopic surface wetness (MSW). The MSW may favor dry surface biofilm (DSB) formation. DSB formation is similar in other industries, it occurs through the processes of adhesion, production of extracellular polymeric substances, development of microcolonies and maturation, it is mediated by a quorum sensing (QS) system and is followed by dispersal, leading to disaggregation. Species that survive on dry surfaces develop tolerance to different stresses. DSB are recalcitrant and contribute to higher resistance to sanitation, becoming potential sources of contamination, related to the spoilage of processed products and foodborne disease outbreaks. In LMF industries, sanitization is performed using physical methods without the presence of water. Although alternative dry sanitizing methods can be efficiently used, additional studies are still required to develop and assess the effect of emerging technologies, and to propose possible combinations with traditional methods to enhance their effects on the sanitization process. Overall, more information about the different technologies can help to find the most appropriate method/s, contributing to the development of new sanitization protocols. Thus, this review aimed to identify the main characteristics and challenges of biofilm management in low moisture food industries, and summarizes the mechanisms of action of different dry sanitizing methods (alcohol, hot air, UV‐C light, pulsed light, gaseous ozone, and cold plasma) and their effects on microbial metabolism.

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Role of fliC on biofilm formation, adhesion, and cell motility in Cronobacter malonaticus and regulation of luxS

Cited by 16 publications

References 54 publications

Uncovering phenotypic changes of luxS‐mediated acid tolerance in Cronobacter malonaticus

Uncovering phenotypic changes of luxS‐mediated acid tolerance in Cronobacter malonaticus

Comparative proteomic and phenotypic changes of LuxS‐mediated hyperosmotic tolerance in Cronobacter malonaticus

Dry surface biofilms in the food processing industry: An overview on surface characteristics, adhesion and biofilm formation, detection of biofilms, and dry sanitization methods

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