Role of GABAA and GABAB Receptors in the Processes Forming Evoked Potentials in Rat Somatosensory Cortex Barrels

Матухно, А. Е.; Sukhov, A. G.; Sinitsyna, V. V.

doi:10.1007/s11055-013-9843-8

Cited by 1 publication

(1 citation statement)

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“…GABARs are classi ed into three types: GABAARs, GABABRs, and GABACRs. The inhibitory effects of GABA are mediated primarily by GABAARs, and it is currently believed that their molecular mechanism involves binding to a speci c recognition site on GABAARs to enhance their inhibitory effects (Matukhno et al 2012). Glu is the most important excitatory neurotransmitter in mammals (Vienne et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Ziwuliuzhu Acupuncture Modulates Glu/GABA‑Gln Metabolic Loop Abnormalities in Insomniac Rats

Huang¹,

Pi²,

Chen³

et al. 2022

Preprint

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Ziwuliuzhu acupuncture is widely considered an effective treatment for insomnia in clinics, but little is known about its possible mechanisms. This study investigated the therapeutic effect of Ziwuliuzhu acupuncture on insomnia and its regulatory mechanism on the glutamic acid (Glu)/γ-aminobutyric acid (GABA)-glutamine (Gln) metabolic loop in a rat model of insomnia. Hematoxylin and eosin staining was used to observe the pathological tissue in the hypothalamus. The levels of the neurotransmitters Glu and GABA in the hypothalamus were determined by high-performance liquid chromatography (HPLC)-mass spectrometry (MS)/MS. Immunohistochemistry (IHC) was used to detect the expression of the GABAA receptor in the hypothalamus. The expression levels of glutamate decarboxylase (GAD65/67) and glutamine synthetase (GS) in the hypothalamus were determined by Western blotting. Compared with the model group, the Ziwuliuzhu acupuncture groups showed decreased Glu levels (p < 0.001) and GABAA receptor expression (p < 0.01), increased GABA levels (p < 0.05), and a decreased Glu/GABA ratio. In comparison to the model group, Ziwuliuzhu acupuncture increased the protein expression of GAD65 (p < 0.001) and GAD67 (p < 0.05) in the hypothalamus and reduced the expression of GS (p < 0.01). Glu/GABA-Gln metabolism may be regulated by Ziwuliuzhu acupuncture to produce sedative and hypnotic effects, affecting Glu and GABA synthesis and decomposition, as well as restoring the excitatory/inhibitory balance between Glu and GABA.

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Section: Discussionmentioning

confidence: 99%