Role of Glucose Variability in the Diagnosis of Septic Patients

Asaduzzaman, Muhammad; Ahmed, Md Nasir Uddin; Rahman, AKM Ferdous; Reza, Syed Tariq; Afifa, Mayisha; Ahmed, BUM Wahid; Asaduzzaman, Mohammad

doi:10.3329/bccj.v10i1.59197

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“…These molecules are 3-aminopropyldimethylethoxysilane (named NH 3 + in this study), n-propyldimethylmethoxysilane (named CH 3 short), octadecyldimethylmetoxysilane (named CH 3 long), and tert -butyl-11-(dimethylamino(dimethyl)silyl)undecanoate (leading to COO – after deprotection). Twenty-five different metabolites (Table ), − with different physicochemical properties (electrical charges and hydrophobic–hydrophilic balance), were analyzed by MALDI-ToF MS. Twenty-three of them are relevant sepsis biomarkers, and two of them are commonly found in the blood. The S/N ratio, STD mean , and intra-substrates uniformity were evaluated as a function of surface chemistry and porosity versus plane substrate.…”

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confidence: 99%

Effect of Silane Monolayers and Nanoporous Silicon Surfaces on the Matrix-Assisted Laser Desorption Ionization Mass Spectrometry Detection of Sepsis Metabolites Biomarkers Mixed in Solution

et al. 2023

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Matrix-assisted laser desorption ionization time-offlight mass spectrometry (MALDI-ToF MS) is a promising strategy for clinical diagnosis based on metabolite detection. However, several bottlenecks (such as the lack of reproducibility in analysis, the presence of an important background in low-mass range, and the lack of organic matrix for some molecules) prevent its transfer to clinical cases. These limitations can be addressed by using nanoporous silicon surfaces chemically functionalized with silane monolayers. In the present study, sepsis metabolite biomarkers were used to investigate the effects of silane monolayers and porous silicon substrates on MALDI-ToF MS analysis (signal-tonoise value (S/N), relative standard deviation of the S/N of triplicate samples (STD mean ), and intra-substrates uniformity). Also, the impact of the physicochemical properties of metabolites, with different isoelectric points and hydrophobic−hydrophilic balances, was assessed. Four different silane molecules, with various alkyl chain lengths and head-group charges, were self-assembled in monolayers on plane and porous silicon surfaces. Their surface coverage and conformity were investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The seven metabolites detected on the stainless-steel target plate (lysophosphatidylcholine, caffeine, phenylalanine, creatinine, valine, arginine, and glycerophosphocholine) are also detected on the silanized and bare, plane and porous silicon surfaces. Moreover, two metabolites, glycine and alanine, which are not detected on the stainless-steel target plate, are detected on all silanized surfaces, except glycine which is not detected on CH 3 short-modified porous silicon and on the bare plane silicon substrate. In addition, whatever the metabolites (except phenylalanine and valine), at least one of the silicon surfaces allows to increase the S/N value in comparison with the stainless-steel target plate. Also, the heterogeneity of matrix crystallization features is linked to the STD mean which is poor on the NH 3 + monolayer on plane substrate and better on the NH 3 + monolayer on porous substrate, for most of the metabolites. Nevertheless, matrix crystallization features are not sufficient to systematically get high STD mean and uniformity in MALDI-ToF MS analysis. Indeed, the physicochemical properties of metabolites and surfaces, limitations in metabolite extraction from the pores, and improvement in metabolite desorption due to the pores are shown to significantly impact MS analysis. In particular, in the case of the most hydrophobic metabolites studied, the highest S/N values and the best STD mean and uniformity (the lowest values) are reached by using porous substrates, while in the case of the most hydrophilic metabolites studied, plane substrates demonstrated the highest S/ N and the lowest STD mean . No clear trend of surface chemistry was evidenced.

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