Role of HSP27 in tumor necrosis factor-α-stimulated interleukin-6 synthesis in osteoblasts

Kato, Kenji; Tokuda, Harukuni; Mizutani, Jun; Adachi, Seiji; Matsushima‐Nishiwaki, Rie; Natsume, Hideo; Kozawa, Osamu; Otsuka, Takanobu

doi:10.3892/ijmm.2011.762

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…Phosphorylated HSP27 was shown to participate in TNF-α-induced IL-6 synthesis in osteoblast-like MC3T3-E1 cells [111]. Overexpression of the phosphomimetic HSP27-3D mutant (in which serine residues 15, 78 and 82 are replaced by aspartic acid) resulted in higher IL-6 mRNA and protein levels than in cells overexpressing the non-phosphorylatable HSP27-3A mutant (in which serine residues 15, 78 and 82 are replaced by alanine).…”

Section: Mk2 and Inflammationmentioning

confidence: 99%

The Role of Mitogen-Activated Protein Kinase-Activated Protein Kinases (MAPKAPKs) in Inflammation

Moens

Kostenko

Sveinbjørnsson

2013

Genes

184

116

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Mitogen-activated protein kinase (MAPK) pathways are implicated in several cellular processes including proliferation, differentiation, apoptosis, cell survival, cell motility, metabolism, stress response and inflammation. MAPK pathways transmit and convert a plethora of extracellular signals by three consecutive phosphorylation events involving a MAPK kinase kinase, a MAPK kinase, and a MAPK. In turn MAPKs phosphorylate substrates, including other protein kinases referred to as MAPK-activated protein kinases (MAPKAPKs). Eleven mammalian MAPKAPKs have been identified: ribosomal-S6-kinases (RSK1-4), mitogen- and stress-activated kinases (MSK1-2), MAPK-interacting kinases (MNK1-2), MAPKAPK-2 (MK2), MAPKAPK-3 (MK3), and MAPKAPK-5 (MK5). The role of these MAPKAPKs in inflammation will be reviewed.

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Section: Mk2 and Inflammationmentioning

confidence: 99%

The Role of Mitogen-Activated Protein Kinase-Activated Protein Kinases (MAPKAPKs) in Inflammation

Moens

Kostenko

Sveinbjørnsson

2013

Genes

184

116

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“…The 27 kDa heat shock protein (Hsp27) is expressed in a differentiation-related pattern and can regulate osteocalcin synthesis ( 21 ). Activating transcription factor 4 (Atf4) is an important transcription factor that serves a pivotal role in osteoblastic differentiation and bone formation ( 22 , 23 ).…”

Section: Introductionmentioning

confidence: 99%

MgCl2 promotes mouse mesenchymal stem cell osteogenic differentiation by activating the p38/Osx/Runx2 signaling pathway

Xiong

2020

Mol Med Rep

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Magnesium, an important inorganic mineral component in bones, enhances osteoblast adhesion and osteogenic gene expression. Mg 2+-containing hydroxyapatite promotes mouse mesenchymal stem cell (MMSc) osteogenic differentiation. in the present study, MMScs were cultured in media containing different concentrations of Mgcl 2 (0 and 20 mM) for different time periods. Western blotting and reverse transcription-quantitative Pcr were performed to determine the expression levels of phosphorylated (p)-p38 mitogen-activated protein kinase (MAPK), the osteoblast-specific transcription factor osterix (osx), runt-related transcription factor 2 (runx2), and p38 downstream genes, such as 27 kda heat shock protein (hsp27), activating transcription factor 4 (atf4), myocyte enhancer factor 2c (Mef2c) and ccaaT/enhancer-binding protein homologous protein (ddit3). The facilitatory effect of Mgcl 2 on MMSc osteogenic differentiation was assessed via alizarin red staining. The results suggested that Mgcl 2 increased p38 phosphorylation compared with the control group. downstream genes of the p38 signaling pathway, including osx and runx2, as well as several osteogenesis-associated downstream target genes, including Hsp27, atf4, ddit3 and Mef2c, were significantly upregulated in the Mg 2+-treated group compared with the control group. The increased osteogenic differentiation in the Mg 2+-treated group was significantly attenuated in MMSCs treated with SB203580, a specific inhibitor of the p38 signaling pathway. The results suggested that appropriate concentrations of Mgcl 2 promoted MMSc osteogenic differentiation via regulation of the p38/osx/runx2 signaling pathway.

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“…Tumor necrosis factor alpha (TNF-α) produced during acute inflammation and it helps in developing resistance to infection and cancers. HSP27 regulates TNF-α-stimulated IL-6 synthesis in osteoblast cells like MC3T3-E1 [11].…”

Section: Hsp27/hspb1mentioning

confidence: 99%

Computational Analysis of Heat shock Protein 27 (HSP27) from different source organisms

Afzal,

Bukhari,

Pervez

et al. 2022

VFAST trans. softw. eng.

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Heat shock protein 27 (HSP27) also called HSPB1 is a member of small heat shock protein (sHsps). HSB1 helps in developing a stable state during stress conditions like heat shock or oxidative injuries. Several studies have performed for the identification of HSPB1 function. However, limitation to highlight structural and phylogenetic relationships of HSPB1 in various organisms. Therefore, the aim of this study to investigate HSPB1 protein in twelve different organisms namely Homo Sapiens (Human), Dugesia japonica (Flatworms), Sus scrofa (Pig), Carassius auratus (Goldfish), Oreochromis niloticus (Nile Tilapia), Rattus norvegicus (Norway Rat), Xenopus laevis (African clawed Frog), Canis lupus familiaris (Dog), Musca domestica (House Fly), Phenacoccus solenopsis (Solenopsis mealybug), Kryptolebias marmoratus (mangrove rivulus\fish) and Alligator mississippiensis (American alligator) by identifying and analyzing the Multiple sequence alignment (MSA), similarity matrix, physiochemical properties, phylogenetic relationship, secondary and tertiary structure, predict motifs and domains, analyze gene structure through several tools..For this purpose organisms were predicted based on soluble and hydrophilic in nature.Results showed all organisms had upstream, downstream and CDS part in their protein sequences except Kryptolebias marmoralus (fish), Oreochromis Niloticus (Nile Tilapia),Phenacoccus Solenopsis (Solenopsis mealybug), and Sus Scrofa (Pig) which had only CDS part. The main domain that was found in all organisms except Phenacoccus were A-Crystalline(IPR002068) and the homologous super family in all organisms were Hsp20 (IPR002068).In phylogenetic tree two clades formed and in the endidentified that Rattus norvegicus and Canis lupus are more similar with each other as they share many common features. Moreover Homo Sapiens, Sus scrofa and Canis lupus, Sus scrofa were found to be more similar. By performing different analysis it was predicted that Phenacoccus Solenopsis was not related to any organism in many aspects. However,limitation to predict quaternary structures of organisms. The Results shows HSPB1 gene has identical homologue, functional similarity and highly conserved among these organisms.

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Role of HSP27 in tumor necrosis factor-α-stimulated interleukin-6 synthesis in osteoblasts

Cited by 4 publications

References 41 publications

The Role of Mitogen-Activated Protein Kinase-Activated Protein Kinases (MAPKAPKs) in Inflammation

The Role of Mitogen-Activated Protein Kinase-Activated Protein Kinases (MAPKAPKs) in Inflammation

MgCl2 promotes mouse mesenchymal stem cell osteogenic differentiation by activating the p38/Osx/Runx2 signaling pathway

Computational Analysis of Heat shock Protein 27 (HSP27) from different source organisms

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