Role of IL-1β and corticosteroids in the regulation of the C/EBP-α, β and δ genes in vivo

Magalini, Alba; Savoldi, G.; Ferrari, Fábio; Garnier, Martine; Ghezzi, Pietro; Albertini, Alberto; Lorenzo, Diego Di

doi:10.1006/cyto.1995.0090

Cited by 26 publications

(27 citation statements)

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“…The gel shift using the C/EBP probe detected no endogenous C/EBP in nuclear extracts, but a significant gel shift was observed after cells were stimulated by either PAO1 or IL-1 ␤ . The activation of this transcription factor was also somewhat diminished by pretreatment of the IB3 cells with dexamethasone, as has been noted in other cell types (37).…”

Section: Activation Of Nf-b In Cf Cellssupporting

confidence: 77%

Activation of NF-kappaB by adherent Pseudomonas aeruginosa in normal and cystic fibrosis respiratory epithelial cells.

DiMango¹,

Ratner²,

Bryan³

et al. 1998

J. Clin. Invest.

290

217

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PMN-dominated airway inflammation is a major component of cystic fibrosis (CF) lung disease. Epithelial cells respond to organisms such as Pseudomonas aeruginosa, the major pathogen in CF, by expressing the leukocyte chemokine IL-8. Experiments were performed using several different types of respiratory epithelial cells that demonstrate that ligation of ceramide-associated receptors on epithelial surfaces by P. aeruginosa pili is a major stimulus for the translocation of transcription factor nuclear factor (

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Section: Activation Of Nf-b In Cf Cellssupporting

confidence: 77%

Activation of NF-kappaB by adherent Pseudomonas aeruginosa in normal and cystic fibrosis respiratory epithelial cells.

DiMango¹,

Ratner²,

Bryan³

et al. 1998

J. Clin. Invest.

290

217

View full text Add to dashboard Cite

show abstract

“…The magnitude of the induction was strikingly higher for C/EBP-␦ in adrenalectomized animals, indicating a suppressive effect of corticosteroids in the IL-1␤ regulatory pathway. For C/EBP-␣, protein synthesis was higher than expected in IL-1␤-treated adrenalectomized animals compared with normal rats (Magalini et al, 1995).…”

Section: The Role Of the Hypothalamic-pituitary-adrenal Axis In The Amentioning

confidence: 63%

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

2004

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“…However, the mechanism of this inhibition is unclear. The basic leucine zipper transcription factor C/EBP␤ belongs to the C/EBP) family and is reported to be induced by IL-1␤ in rat liver (25). To investigate whether C/EBP␤ can play a role in the repression of ␣-SMA gene expression by IL-1␤, the expression of C/EBP␤ in rat lung fibroblasts was analyzed by Western blot analysis.…”

Section: Effects Of Il-1␤ On Fibroblast C/ebp␤ Expressionmentioning

confidence: 99%

CCAAT/Enhancer-Binding Protein β Isoforms and the Regulation of α-Smooth Muscle Actin Gene Expression by IL-1β

Jin

et al. 2004

The Journal of Immunology

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The role of IL-1β in inflammation is amply documented, but its ability to inhibit myofibroblast differentiation and, in particular, the suppression of α-smooth muscle actin (α-SMA) gene expression is less well understood. Because IL-1β can induce C/EBPβ expression, the role of C/EBPβ isoforms in IL-1β regulation of α-SMA gene expression was investigated in rat lung myofibroblasts. The results showed that IL-1β inhibited α-SMA expression in a dose-dependent manner, which was associated with stimulation of the expression of both C/EBPβ isoforms, liver-enriched activating protein (LAP) and liver-enriched inhibitory protein (LIP). However, a greater increase in LIP relative to LAP expression resulted in a reduced LAP/LIP ratio after IL-1β treatment. Transfection with an LAP-expressing plasmid stimulated, whereas an LIP-expressing plasmid inhibited, α-SMA expression. Cells from C/EBPβ-deficient mice had reduced levels of α-SMA expression and promoter activity, which failed to respond to IL-1β treatment. Sequence analysis identified the presence of a C/EBPβ consensus binding sequence in the α-SMA promoter, which, when mutated, resulted in diminished promoter activity and abolished its responsiveness to IL-1β treatment. EMSA revealed binding of C/EBPβ to this C/EBPβ consensus binding sequence from the α-SMA promoter. Finally, IL-1β enhanced the expression of eukaryotic initiation factor 4E, a stimulator of LIP expression, which may account for a mechanism by which IL-1β could alter the LAP/LIP ratio. These data taken together suggest that C/EBPβ isoforms regulate α-SMA gene expression, and that its inhibition by IL-1β was due to preferential stimulation of LIP expression.

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Role of IL-1β and corticosteroids in the regulation of the C/EBP-α, β and δ genes in vivo

Cited by 26 publications

References 0 publications

Activation of NF-kappaB by adherent Pseudomonas aeruginosa in normal and cystic fibrosis respiratory epithelial cells.

Activation of NF-kappaB by adherent Pseudomonas aeruginosa in normal and cystic fibrosis respiratory epithelial cells.

Liver-Enriched Transcription Factors in Liver Function and Development. Part II: the C/EBPs and D Site-Binding Protein in Cell Cycle Control, Carcinogenesis, Circadian Gene Regulation, Liver Regeneration, Apoptosis, and Liver-Specific Gene Regulation

CCAAT/Enhancer-Binding Protein β Isoforms and the Regulation of α-Smooth Muscle Actin Gene Expression by IL-1β

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