Role of interleukin-6 in inhibiting hepatic autophagy markers in exercised mice

Pinto, Ana P.; Rocha, Aristides Almeida; Cabrera, Elisa; Marafon, Bruno B.; Kohama, Eike B.; Rovina, Rafael L.; Simabuco, Fernando Moreira; Bueno, Carlos; Moura, Leandro Pereira de; Pauli, José Rodrigo; Cintra, Dennys Esper; Ropelle, Eduardo R.; Silva, Adelino Sánchez Ramos da

doi:10.1016/j.cyto.2020.155085

Cited by 11 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Reinforcing the findings with hepatic cells (55,63), IL-6 KO mice demonstrated lower mRNA levels of autophagic markers in the liver at baseline (54). After an acute exhaustive exercise session, hepatic tissue in WT mice displayed a reduction of some autophagy-related mRNA and protein levels; however, these results were attenuated or absent in IL-6 KO mice (54).…”

Section: Emerging Roles Of Il-6 and Irisin In The Activation Of Liver...supporting

confidence: 73%

Physical Exercise and Liver Autophagy: Potential Roles of IL-6 and Irisin

Pinto

Ropelle

Quadrilatero

et al. 2021

Exercise and Sport Sciences Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Emerging Roles Of Il-6 and Irisin In The Activation Of Liver...supporting

confidence: 73%

Physical Exercise and Liver Autophagy: Potential Roles of IL-6 and Irisin

Pinto

Ropelle

Quadrilatero

et al. 2021

Exercise and Sport Sciences Reviews

Self Cite

View full text Add to dashboard Cite

show abstract

“…Recently, the role of IL-6 on liver autophagy has been investigated under physical exercise condition, showing that IL-6 KO mice have inhibited some hepatic autophagy markers at baseline (i.e., ULK1 and Atg5). The acute exhaustive exercise reduced mRNA levels of genes related to the autophagy pathway (e.g., FOXO1a) in the livers of WT mice; however, these effects were attenuated for the IL-6 KO group, highlighting a new function of this cytokine [ 131 ].…”

Section: Myokines and Autophagymentioning

confidence: 99%

Irisin and Autophagy: First Update

Pesce

Ballerini

Paolucci

et al. 2020

IJMS

View full text Add to dashboard Cite

Aging and sedentary life style are considered independent risk factors for many disorders. Under these conditions, accumulation of dysfunctional and damaged cellular proteins and organelles occurs, resulting in a cellular degeneration and cell death. Autophagy is a conserved recycling pathway responsible for the degradation, then turnover of cellular proteins and organelles. This process is a part of the molecular underpinnings by which exercise promotes healthy aging and mitigate age-related pathologies. Irisin is a myokine released during physical activity and acts as a link between muscles and other tissues and organs. Its main beneficial function is the change of subcutaneous and visceral adipose tissue into brown adipose tissue, with a consequential increase in thermogenesis. Irisin modulates metabolic processes, acting on glucose homeostasis, reduces systemic inflammation, maintains the balance between resorption and bone formation, and regulates the functioning of the nervous system. Recently, some of its pleiotropic and favorable properties have been attributed to autophagy induction, posing irisin as an important regulator of autophagy by exercise. This review article proposes to bring together for the first time the “state of the art” knowledge regarding the effects of irisin and autophagy. Furthermore, treatments on relation between exercise/myokines and autophagy have been also achieved.

show abstract

“…35,36 Another study verified that IL-6 knockout (KO) mice demonstrated lower levels of autophagic markers in the liver at baseline. 37 Therefore, the lower concentrations of IL-6 protein could justify the lower autophagic mRNA levels visualized for the OT/Rapa group.…”

Section: Discussionmentioning

confidence: 99%

Chronic rapamycin treatment decreases hepatic IL‐6 protein, but increases autophagy markers as a protective effect against the overtraining‐induced tissue damage

Pinto

Rocha

Marafon

et al. 2022

Clin Exp Pharma Physio

Self Cite

View full text Add to dashboard Cite

Regular endurance exercise is a non‐pharmacological strategy to protect the liver against diseases. Conversely, exercise may be harmful when excessive, the so‐called overtraining. As expected, mice who underwent an overtraining protocol presented higher levels of proinflammatory cytokines in the serum and liver. Based on the relationship among overtraining, inflammation and mammalian target of rapamycin complex 1 (mTORC1) upregulation, the present study verified if animals submitted to an overtraining protocol, but with inhibition of the mTOR pathway via rapamycin injections could mitigate the liver and serum inflammation. Once autophagy can be linked to the improvement of hepatic dysfunction, we also investigated if the inhibition of mTORC1 by rapamycin can improve hepatic autophagy. The animals were randomized into four groups: control (CT; sedentary mice), overtraining by downhill running (OT; mice submitted to the downhill running‐based overtraining protocol), overtraining by downhill running with chronic administration of rapamycin (OT/Rapa; mice submitted to the downhill running‐based overtraining protocol with intraperitoneal injections of rapamycin) and aerobic (AER; submitted to aerobic training protocol). The serum and liver of the animals were used for biochemical analysis, reverse transcription‐quantitative polymerase chain reaction (RT‐qPCR) and immunoblotting. The main results are (a) OT and OT/Rapa protocols decreased the performance; (b) the protein levels of interleukin 6 (IL‐6) were higher for the OT group; the OT/Rapa group reduced the autophagic genes, increased the microtubule‐associated protein light chain 3 II/I (LC3II/LC3I) protein ratio and decreased the sequestosome 1 (SQSTM1) protein. In conclusion, rapamycin appears efficiently to increase the autophagy proteins and decrease IL‐6 protein in the liver of overtraining mice.

show abstract

Role of interleukin-6 in inhibiting hepatic autophagy markers in exercised mice

Cited by 11 publications

References 47 publications

Physical Exercise and Liver Autophagy: Potential Roles of IL-6 and Irisin

Physical Exercise and Liver Autophagy: Potential Roles of IL-6 and Irisin

Irisin and Autophagy: First Update

Chronic rapamycin treatment decreases hepatic IL‐6 protein, but increases autophagy markers as a protective effect against the overtraining‐induced tissue damage

Contact Info

Product

Resources

About