Role of ions and ion channels in the regulation of Atlantic croaker sperm motility

Detweiler, Charles; Thomas, P. C.

doi:10.1002/(sici)1097-010x(19980601)281:2<139::aid-jez8>3.3.co;2-o

Cited by 6 publications

(9 citation statements)

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“…The pH of the diluents was adjusted to 8.2 using 1 mol/L NaOH. In the sperm of carp Cyprinus carpio and the Atlantic croaker Micropogonias undulatus , the effective concentration of 4‐AP (to produce an inhibitory effect) has been reported as 1–5 m m 18,19 . In the present study, the concentration of 4‐AP was determined following the reports of Krasznai et al.…”

Section: Methodsmentioning

confidence: 66%

“…In the present study, the concentration of 4‐AP was determined following the reports of Krasznai et al. 18 and Detweiler and Thomas 19 . The motility of sperm from five or six males was measured in each diluent.…”

Section: Methodsmentioning

confidence: 97%

“…To investigate the role of NaHCO 3 , various reagents were selected that could regulate the internal factors mentioned above. The voltage‐dependent K + channel blocker 4‐aminopyridine (4‐AP) is a candidate for this purpose, 13–16 because K + plays an important role in the initiation of fish sperm motility, 4,17 and the inhibitory effects of 4‐AP on the function of the potassium channel have been reported 18,19 . Moreover, a therapeutic effect of 4‐AP has been used in human medical treatment, and its effect is enhanced by the presence of NaHCO 3 , suggesting that NaHCO 3 is involved in K + transport 20 .…”

Section: Introductionmentioning

confidence: 99%

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Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Tanaka¹,

Utoh²,

Yamada³

et al. 2004

Fisheries Sci

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In order to find out the role of sodium bicarbonate (NaHCO 3 ) on the initiation of sperm motility in the Japanese eel Anguilla japonica , interactions were investigated between NaHCO 3 and various reagents (K + channel blocker 4-aminopyridine [4-AP], ammonium chloride [NH 4 Cl], sodium acetate and calcium chloride [CaCl 2 ]) that could regulate internal factors (intracellular K + , intracellular pH [[pH] i ] and intracellular Ca 2 + ) in sperm motility. Contradictory effects of NaHCO 3 were observed (i.e. an inhibitory effect when 4-AP was absent and a promoting effect when 4-AP was present). Sodium bicarbonate inhibited the initiation of sperm motility in the Japanese eel. However, NaHCO 3 restored the motility of immotile sperm that 4-AP inhibited. The inhibitory effect of NaHCO 3 disappeared with the addition of NH 4 Cl, which raised [pH] i , but the promoting effect was not affected by [pH] i . Although NaHCO 3 recovered motility in the presence of 4-AP, this recovery was also observed with the addition of CaCl 2 instead of NaHCO 3 . In the initiation of sperm motility in the Japanese eel, two roles for NaHCO 3 are suggested: an inhibitory role relating to the regulation of [pH] i and a promoting role relating to the uptake of another initiation factor, which could be Ca 2 + .

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Section: Methodsmentioning

confidence: 66%

Section: Methodsmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Tanaka¹,

Utoh²,

Yamada³

et al. 2004

Fisheries Sci

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“…The osmolality of Atlantic croaker milt was previously measured at 337.5 ± 8.5 mOsm kg À1 (Detweiler & Thomas 1998). Hank's balanced salt solution at 200 mOsm kg À1 (HBSS 200;Wayman et al 1998) was used as hypotonic dilution medium known to prevent sperm activation in marine species.…”

Section: Experiments 1: Estimation Of Sperm Concentration By Spectrophmentioning

confidence: 99%

“…This popular baitfish and food fish species supports a significant and seasonal fishery (US$8.6 million in the US; National Marine Fisheries Service 2011) and is recently emerging as a strong candidate for multi-purpose aquaculture (Sink, Strange & Lochmann 2010). It is also a convenient model-species previously used in, e.g., fundamental reproductive physiology (Detweiler & Thomas 1998), eco-toxicology and reproductive disruption (Thomas & Rahman 2010), development of sperm cryopreservation protocols (Gwo & Arnold 1992) and wild population-dynamic models (Hare, Alexander, Fogarty, Williams & Scott 2010).…”

Section: Introductionmentioning

confidence: 99%

Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croakerMicropogonias undulatusL. broodstock

et al. 2012

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The objective of this study was to optimize the methodology for spectrophotometric determination of sperm concentration in Atlantic croaker Micropogonias undulatus L. milt and to estimate its potential for short-term cold-storage. The spectrophotometric determination of sperm concentration was evaluated using milt samples from six males serially diluted in Hank's balanced salt solution at 200 mOsm kg À1 (HBSS). The predictive power of regression models between sperm concentration and absorbance was determined from 200 to 500 nm and found to be highest within the visible spectrum despite a peak of milt absorbance at 288 nm. Absorbance reading at 400 nm was selected for further analysis to maximize the absorbance of the sample hence the sensitivity of the method while minimizing the impact of potential sample contamination with blood. The standard-curve of correlation between sperm absorbance at 400 nm and concentration was validated and held an accuracy ranging between À7.40% and +4.56% across males. Total sperm motility duration and the proportion of motile spermatozoa were significantly higher in milt samples diluted 1:3 in HBSS than in the undiluted control during up to 30 h of cold-storage.The methodologies investigated in this study can be applied to optimize sperm usage and achieve predictable artificial fertilization protocols in Atlantic croaker.

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Sperm motility in the steelhead Oncorhynchus mykiss (Walbaum): influence of the composition of the incubation and activation media

et al. 2006

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This study examined the pH sensitivity of steelhead, Oncorhynchus mykiss (Walbaum), sperm motility relative to the composition of incubation and activation media. The percentage of sperm that initiated motility following incubation in a sperm immobilizing solution (SI) titrated to different pH values and subsequent activation by dilution in buffered swimming medium (SM) at pH 8.5 or 50% ovarian fluid (OF) showed little or no pH sensitivity; sperm diluted in de‐ionized water (DI) showed no motility after incubation at any pH. In contrast, motility of sperm diluted in tap water (TAP) was highly sensitive to the pH of the incubation medium. Sperm incubated with buffered seminal plasma at high, but not low pH demonstrated high percent motility when diluted with DI. Sperm incubated in low‐pH SI demonstrated high motility only when diluted into high‐pH SM. The effects of the composition of incubation and activation media on sperm motility were generally reflected in comparable effects on fertility. Therefore, these data indicate that the pH sensitivity of sperm motility and fertility depends on the composition of commonly used incubation as well as activation media.

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Role of ions and ion channels in the regulation of Atlantic croaker sperm motility

Cited by 6 publications

References 0 publications

Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Role of sodium bicarbonate on the initiation of sperm motility in the Japanese eel

Spectrophotometric determination of sperm concentration and short-term cold-storage of sperm in Atlantic croakerMicropogonias undulatusL. broodstock

Sperm motility in the steelhead Oncorhynchus mykiss (Walbaum): influence of the composition of the incubation and activation media

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