Role of lipocalin 2 in stroke

Zhao, Ruoyu; Wei, Pengju; Sun, Xin; Zhang, Dianhui; He, Qianyan; Liu, Jie; Chang, Junlei; Yang, Yi; Guo, Zhen‐Ni

doi:10.1016/j.nbd.2023.106044

Cited by 26 publications

(11 citation statements)

References 196 publications

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“…Although this is not an MC medium condition-specific event, LCN2 expression was only induced upon IL-1β stimulation when the cells were in a spheroid state. Astrocyte-derived LCN2 is involved in chronic itch [ 37 ], cerebral stroke [ 38 , 39 ] and so on. To reveal the role of LCN2 in these diseases, IL-1β-dependent and TNF-α independent induction mechanism of the of HASTR/ci35 spheroids might be a useful tool.…”

Section: Resultsmentioning

confidence: 99%

Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages

et al. 2023

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Astrocytes, a type of glial cell in the brain, are thought to be functionally and morphologically diverse cells that regulate brain homeostasis. Cell immortalization is a promising technique for the propagation of primary human astrocytes. The immortalized cells retain their astrocytic marker mRNA expression at lower levels than the primary cells. Therefore, improvement of the differentiation status is required. The use of a 3D formation technique to mimic structural tissue is a good strategy for reflecting physiological cell–cell interactions. Previously, we developed a spheroid formation method using highly viscous methyl cellulose (MC) medium. In this study, we applied this formation method to the well-established immortalized human astrocyte cell line HASTR/ci35. Stable HASTR/ci35 spheroids were successfully formed in MC medium, and laminin deposition was detected inside of the spheroids. Their functional markers were enhanced compared to conventional spheroids formed in U-bottom plates. The inflammatory response was moderately sensitive, and the ability to support neurite growth was confirmed. The HASTR/ci35 spheroid in the MC medium demonstrated the differentiation phenotype and could serve as a potent in vitro model for matured astrocytes.

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Section: Resultsmentioning

confidence: 99%

Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages

et al. 2023

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“…Increased expression of LCN2 favors the occurrence of inflammation. [30] Research indicates that the transcription factor FRA-1/ AP-1 controls the expression of lipocalin-2 and inflammation in a sepsis model, and FRA-1 may play a role in modulating LCN2 in sepsis tolerance response. [31] Plasma Hsa-miR-92a-3p is associated with LCN2 and is related to coagulation dysfunction in sepsis.…”

Section: Discussionmentioning

confidence: 99%

LCN2 and ELANE overexpression induces sepsis

Wang,

Han,

Dong

et al. 2024

Medicine

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Sepsis is a syndrome characterized by a systemic inflammatory response due to the invasion of pathogenic microorganisms. The relationship between Lipocalin-2 (LCN2), elastase, neutrophil expressed (ELANE) and sepsis remains unclear. The sepsis datasets GSE137340 and GSE154918 profiles were downloaded from gene expression omnibus generated from GPL10558. Batch normalization, differentially expressed Genes (DEGs) screening, weighted gene co-expression network analysis (WGCNA), functional enrichment analysis, Gene Set Enrichment Analysis (GSEA), immune infiltration analysis, construction and analysis of protein–protein interaction (PPI) networks, Comparative Toxicogenomics Database (CTD) analysis were performed. Gene expression heatmaps were generated. TargetScan was used to screen miRNAs of DEGs. 328 DEGs were identified. According to Gene Ontology (GO), in the Biological Process analysis, they were mainly enriched in immune response, apoptosis, inflammatory response, and immune response regulation signaling pathways. In cellular component analysis, they were mainly enriched in vesicles, cytoplasmic vesicles, and secretory granules. In Molecular Function analysis, they were mainly concentrated in hemoglobin binding, Toll-like receptor binding, immunoglobulin binding, and RAGE receptor binding. In Kyoto Encyclopedia of Genes and Genomes (KEGG), they were mainly enriched in NOD-like receptor signaling pathway, Toll-like receptor signaling pathway, TNF signaling pathway, P53 signaling pathway, and legionellosis. Seventeen modules were generated. The PPI network identified 4 core genes (MPO, ELANE, CTSG, LCN2). Gene expression heatmaps revealed that core genes (MPO, ELANE, CTSG, LCN2) were highly expressed in sepsis samples. CTD analysis found that MPO, ELANE, CTSG and LCN2 were associated with sepsis, peritonitis, meningitis, pneumonia, infection, and inflammation. LCN2 and ELANE are highly expressed in sepsis and may serve as molecular targets.

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“…NGAL was originally identi ed in neutrophils and is mainly and is mainly produced by injured nephron epithelia, but it is also produced by macrophages, activated leukocytes, the choroid plexus, and many other tissues [28,29]. Besides being a biomarker of renal injury, more and more evidence has been reported that NGAL is an acute-phase protein and an actor of innate immunity, especially during the early stages of infection.…”

Section: Discussionmentioning

confidence: 99%

Diagnostic value of cerebrospinal fluid Neutrophil Gelatinase-Associated Lipocalin for differentiation of bacterial meningitis from tuberculous meningitis or cryptococcal meningitis: a prospective cohort study

Wang

Lin

Wang

et al. 2023

Preprint

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Background The early differential diagnosis between bacterial meningitis (BM) and tuberculous meningitis (TBM) or cryptococcal meningitis (CM) is still an important clinical challenge. Neutrophil Gelatinase-Associated Lipocalin (NGAL) had been reported as a novel inflammatory biomarker in early stage of infection. This study aimed to explore whether cerebrospinal fluid (CSF) NGAL is a potential biomarker for distinguishing between BM and TBM or CM. Methods Prospectively enrolled the patients with suspected CNS infections at admission and divided them into three case groups: BM (n = 67), TBM (n = 55), CM (n = 51), and an age- and sex-matched hospitalized control (HC, n = 58). Detect the CSF NGAL and evaluate the diagnostic accuracy to distinguish between BM and TBM or CM. Meanwhile, longitudinally measured the CSF NGAL of BM to evaluate whether it would be a monitor of antibacterial treatment. Results The CSF NGAL in BM was significantly higher than in TBM, CM and HC (all P < 0.05), while the serum NGAL was not significantly different among the three case groups. The ROC analysis showed CSF NGAL presented a good diagnostic performance with an AUC of 0.834 (0.770 to 0.886) and at the optimal cutoff value of 74.27 ng/mL with 70.15% sensitivity and 77.36% specificity for discriminating BM with TBM and CM. Meanwhile, the CSF NGAL in the convalescent period of BM was significantly lower than the acute period (P < 0.05). Conclusions CSF NGAL might be a potential biomarker for distinguishing between acute BM and TBM or CM, and it also has some important clinical value for monitoring antibiotic therapy for BM.

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Role of lipocalin 2 in stroke

Cited by 26 publications

References 196 publications

Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages

Rapid and Stable Formation Method of Human Astrocyte Spheroid in a High Viscous Methylcellulose Medium and Its Functional Advantages

LCN2 and ELANE overexpression induces sepsis

Diagnostic value of cerebrospinal fluid Neutrophil Gelatinase-Associated Lipocalin for differentiation of bacterial meningitis from tuberculous meningitis or cryptococcal meningitis: a prospective cohort study

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