1992
DOI: 10.1016/0005-2760(92)90015-n
|View full text |Cite
|
Sign up to set email alerts
|

Role of lipoprotein-copper complex in copper catalyzed-peroxidation of low-density lipoprotein

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1

Citation Types

3
56
0
1

Year Published

1997
1997
2010
2010

Publication Types

Select...
8

Relationship

1
7

Authors

Journals

citations
Cited by 143 publications
(60 citation statements)
references
References 43 publications
3
56
0
1
Order By: Relevance
“…We assume that half saturation of the high affinity sites occurs in range of 5 to 100 copper/LDL, while half saturation of the low affinity binding sites requires much higher copper, in range of 200 to 500 copper/LDL. The assumption of two copper binding sites is consistent with previous investigation by Kuzuya et al (17). The rapid acceleration of the second propagation phase can be explained by the decomposition of hydroperoxides by copper ions (18).…”
Section: Resultssupporting
confidence: 91%
“…We assume that half saturation of the high affinity sites occurs in range of 5 to 100 copper/LDL, while half saturation of the low affinity binding sites requires much higher copper, in range of 200 to 500 copper/LDL. The assumption of two copper binding sites is consistent with previous investigation by Kuzuya et al (17). The rapid acceleration of the second propagation phase can be explained by the decomposition of hydroperoxides by copper ions (18).…”
Section: Resultssupporting
confidence: 91%
“…LDL was oxidized by incubating 500 g/mL LDL in 10 mol/L CuSO 4 for 16 hours at 37°C and then dialyzed in PBS containing 0.1 mmol/L EDTA for 24 hours at 4°C, followed by further dialysis against PBS for 24 hours at 4°C to exclude EDTA as previously described. 27,28 Fresh preparations of LDL and Ox-LDL were used for each experiment.…”
Section: Ldl Preparationmentioning
confidence: 99%
“…66,67 Column chromatography gives very low estimates of copper binding to LDL. 24,26,30 There is likely to be a problem with this technique, inasmuch as copper ions bind to Sephadex during column chromatography. This can be readily seen by passing LDL-copper complexes through Sephadex G-25 PD-10 columns (Pharmacia Biotech) and eluting with BC and ascorbate after the LDL has emerged and the column has been eluted with several times its bed volume.…”
Section: Discussionmentioning
confidence: 99%
“…Using dialysis to separate free from lipoprotein-bound copper in the absence of a bulk pool of copper in the dialysis buffer 26,29 may result in the dissociation of some of the copper from the LDL.…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation