Role of manganese in protection against oxidative stress under iron starvation in cyanobacterium Anabaena 7120

Abstract

The cyanobacterium Anabaena sp. PCC 7120 was grown in presence and absence of iron to decipher the role of manganese in protection against the oxidative stress under iron starvation and growth, manganese uptake kinetics, antioxidative enzymes, lipid peroxidation, electrolyte leakage, thiol content, total peroxide, proline and NADH content was investigated. Manganese supported the growth of cyanobacterium Anabaena 7120 under iron deprived conditions where maximum uptake rate of manganese was observed with lower K(m) and higher V(max) values. Antioxidative enzymes were also found to be elevated in iron-starved conditions. Estimation of lipid peroxidation and electrolyte leakage depicted the role of manganese in stabilizing the integrity of the membrane which was considered as the prime target of oxygen free radicals in oxidative stress. The levels of total peroxide, thiol, proline and NADH content, which are the representative of oxidative stress response in Anabaena 7120, were also showed increasing trends in iron starvation. Hence, the results discerned, clearly suggested the role of manganese in protection against the oxidative stress in cyanobacterium Anabaena 7120 under iron starvation either due to its antioxidative properties or involvement as cofactor in a number of antioxidative enzymes.

Synergic Effects of Temperature and Irradiance on the Physiology of the Marine Synechococcus Strain WH7803

“…In this context, it is also worth noting that genes coding for two manganese transporters, the high-affinity MntABC transport system and another ABC-type Mn 2+ /Zn 2+ transport system (SynWH7803_0988-90) were particularly upregulated under LT stresses, suggesting that like in Anabaena sp. PCC 7120, Mn could also play a role in protection against ROS due to its antioxidative properties or involvement as a cofactor in several antioxidative enzymes ( Kaushik et al, 2015 ).…”

Manganese and iron deficiency in Southern Ocean Phaeocystis antarctica populations revealed through taxon-specific protein indicators

“…Mn can be a cofactor for enzymes with superoxide dismutase activity, scavenging reactive oxygen species (ROS) generated during photosynthesis, especially under Fe-deficient conditions 19 . Indeed, interactions between Mn and Fe demand have been reported for phytoplankton including diatoms as well as cyanobacteria, suggesting an increased biochemical requirement for Mn under low Fe availability 20–24 .…”

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