Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Mokbel, El-Sayed Mohammad Soliman; Swelam, Eman S.; Radwan, Eman M. M.; Kandil, Mohammed A.

doi:10.1515/jppr-2017-0039

Cited by 3 publications

(1 citation statement)

References 14 publications

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“…Due to the high fertility of aphids and their potential for developing resistance to certain insecticides, using pesticides to control their population is not always successful (Abdallah et al, 2016;Mokbel et al, 2017;Mweke et al, 2019). Selective insecticides, such as neonicotinoids, have recently entered the market to take the place of organophosphates and methyl-carbamates as a result of resistance build-up in insect pests (such aphids) to the bulk of conventional insecticides (Tomizawa et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.

Abdelmoteleb,

Mohamed,

Genidy

et al. 2023

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Neonicotinoids, which have a wide range of applications, are systemic insecticides that efficiently control several sucking pests. Recently, thiamethoxam (THIA) has become one of the popular pest control agents. In vivo study, the toxicity of THIA was evaluated against a laboratory strain of cowpea aphid (Aphis craccivora Koch.) using the leaf dipping method for 24 and 48 hrs of exposure. In silico study, THIA was docked to acetylcholine binding protein (AChBP) to explain their binding mode of interaction. THIA exhibited good toxicity against adult aphids with LC50 of 2.44 ppm at 24 hrs post-treatment, which greatly decreased to 0.75 ppm at 48 hrs post-treatment. THIA showed better binding interaction with AChBP than the co-crystalized ligand (imidacloprid) and good alignment in the active site. THIA indicated different variations in the activities of tested enzymes. Where, aphids treated with LC50 of THIA increased the biochemical activities of acetylcholinesterase, antioxidant enzymes and peroxidases while decreasing the activity of alpha esterase, carboxylesterase (CarE), glutathione S-transferase and catalase. Therefore, this compound is efficient against sap-sucking pests with good irreversible and specific binding interaction with nicotinic acetylcholine receptor (nAChR) and, consequently, low mammalian toxicity. So, THIA is considered a favourable agent for the management of insect pests.

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Section: Introductionmentioning

confidence: 99%

Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.

Abdelmoteleb,

Mohamed,

Genidy

et al. 2023

Egyptian Academic Journal of Biological Sciences. A, Entomology

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Susceptibility of the Cowpea Aphid (Hemiptera: Aphididae) to Widely Used Insecticides in Australia

Umina

Reidy‐Crofts

Edwards

et al. 2021

Journal of Economic Entomology

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Globally, 27 aphid species have evolved resistance to almost 100 insecticide active ingredients. A proactive approach to resistance management in pest aphids is needed; this should include risk analysis, followed by regular baseline susceptibility assays for species deemed at high risk of evolving resistance. The cowpea aphid (Aphis craccivora Koch) has evolved insecticide resistance to multiple insecticides outside Australia and was recently identified as a high-risk species in Australia. In this study, we generated toxicity data against four insecticides (representing four unique chemical Mode of Action groups) for populations of A. craccivora collected across Australia. Alpha-cypermethrin was the most toxic chemical to A. craccivora in leaf-dip laboratory bioassays with an average LC50 value across nine populations of 0.008 mg a.i./L, which was significantly lower than dimethoate (1.17 mg a.i./L) and pirimicarb (0.89 mg a.i./L). Small, but significant, differences in sensitivity were detected in some populations against pirimicarb and dimethoate, whereas responses to alpha-cypermethrin and imidacloprid were not significantly different across all aphid populations examined in this study. For all insecticides, the field rate controlled 100% of individuals tested. The data generated will be important for future monitoring of insecticide responses of A. craccivora. Proactive management, including increased reliance on non-chemical pest management approaches and routine insecticide baseline sensitivity studies, is recommended for A. craccivora.

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Toxicity of Essential Oils Nanoemulsion Against Aphis Craccivora and Their Inhibitory Activity on Insect Enzymes

et al. 2021

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Essential oils are widely used as botanical insecticides rather than chemically synthesized pesticides which led to catastrophic effects on humans, the environment, and eutrophication. Here, encapsulation of four essential oils Basilicum ocimum, Cuminum cyminum, Origanum marjorana, and Matricaria chamomilla were utilized in the presence of 3% v/v ethanol, as anti-insect against Aphis craccivora and compared to traditional insecticides dinotefuran and pymetrozine. Different tools were used to characterize the prepared nanoemulsion such as TEM, SEM, and Zeta potential analyzer. Besides, selected B. ocimum and C. cyminum were analyzed by gas chromatography-mass GC/mass spectrometry. The results reveal that nanoemulsion exhibited considerable toxic activities against laboratory and field strains of cowpea aphid. In the toxicity bioassay test of essential oils, moderate mortality was observed at 10,000 mg/L against aphid with lethal concentration that kills 50% of insects (LC50) values of basil 992 mg/L and marjoram 3162 mg/L. Else, nanoemulsion provided the highest mortality rate at 625 mg/L and the LC50 values of basil nanoemulsion (NE) 45 mg/L, and marjoram NE 188 mg/L in laboratory strains. The systemic effects of the tested substances acetylcholine esterase, alkaline phosphatase, β-esterases, glutathione S-transferase (GST), and mixed-function oxidase (MFO) enzymes on insects were found to be significantly decreased and increased when compared with control groups. Overall, these results highlight that the nanoemulsion is potential tools to control cowpea aphid and could be useful in developing integrated insect management in faba bean fields.

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Role of metabolic enzymes in resistance to chlorpyrifos-methyl in the cowpea aphid, Aphis craccivora (Koch)

Cited by 3 publications

References 14 publications

Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.

Computational and Toxicological Evaluation of Thiamethoxam as Nicotinic Acetylcholine Receptor Modulator Against Cowpea Aphid, Aphis craccivora Koch.

Susceptibility of the Cowpea Aphid (Hemiptera: Aphididae) to Widely Used Insecticides in Australia

Toxicity of Essential Oils Nanoemulsion Against Aphis Craccivora and Their Inhibitory Activity on Insect Enzymes

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